Antispasmodic activity of <i>Symplocos paniculata</i> is mediated through opening of ATP-dependent K+ channel

Abstract

&lt;p class="Abstract"&gt;&lt;em&gt;Symplocos paniculata&lt;/em&gt; is a medicinal plant used by native healers to manage gastrointestinal ailments. The crude methanolic extract of &lt;em&gt;S. paniculata&lt;/em&gt; was screened pharmacologically both in vitro and in vivo for the validation of its therapeutic potential. It suppressed the spontaneous activity of isolated rabbit jejunum preparations and also caused inhibition of the low K&lt;sup&gt;+ &lt;/sup&gt;(20 mM)- induced spastic contractions in isolated rabbit jejunum preparations in a manner comparable to cromakalim. The relaxant effect was found to be blocked following glibenclamide exposure of the isolated tissue preparations similar to cromakalim, suggesting that observed response was likely to be mediated through opening of ATP dependent K&lt;sup&gt;+ &lt;/sup&gt;channels. Following oral administration to mice provided protection against castor oil-induced diarrhea in a manner similar to loperamide. The plant material was found safe in toxicity study up to oral dose of 8 g/kg in mice. Hence, present study provides a scientific basis for the vernacular use of &lt;em&gt;S. paniculata&lt;/em&gt; in gastro-intestinal system.&lt;/p&gt;&lt;p&gt; &lt;/p&gt;

The Potential Involvement of an ATP-Dependent Potassium Channel-Opening Mechanism in the Smooth Muscle Relaxant Properties of Tamarix dioica Roxb.

“…An agent that completely reduces the contractions provoked by K + (25 mmol/L) only is denoted as a potassium channel activator, whereas agents that relax both K + (25 mmol/L) and K + (80 mmol/L) are referred to as calcium channel blockers. This test discriminates conclusively between calcium channel blockers and potassium channel activators [26]. Pretreatment of tissue with glibenclamide (K ATP channel antagonist) reduces the concentration‒response curve of TdCr against K + (25 mmol/L), which shows the contribution of the K + ATP channel opener activity.…”

Dose-dependent Spasmolytic, Bronchodilator, and Hypotensive Activities of Panicum miliaceum L.

“…Spasmolytic effect of plant extract may be due to blocking of Ca ++ channels or opening of K + channels. 21 The relaxation effect of plant extract on contractions triggered by Low K + is usually considered due to possible involvement of K + -channel opening mechanism. 22 …”

“…Spasmolytic effect of plant extract may be due to blocking of Ca ++ channels or opening of K + channels. 21 The relaxation effect of plant extract on contractions triggered by Low K + is usually considered due to possible involvement of K + -channel opening mechanism. 22 To determine its K + channel activation, low-K + triggered contraction pretreated with glibenclamide was evaluated with extract doses which inhibited its relaxing effect having similar pattern as cromakalim confirmed its K + -ATP potassium channel opening mechanism.…”

Ethnopharmacological basis for antispasmodic, antidiarrheal and antiemetic activities of Ceratonia siliqua pods

“…To confirm whether it was through potassium channel opening effect, K + (25 mM) was added in tissue bath to attain sustained contraction of the rabbit jejunum tissue. The test material was then added in a cumulative manner to observe concentration -dependent inhibitory response (Janbaz et al, 2016;Rafique et al, 2016).…”