Antiserum and antibiotic in the prophylaxis of burns against pseudomonas aeruginosa

Jones, Rhys; Jackson, Douglas; Lowbury, E. J. L.

doi:10.1016/s0007-1226(66)80007-3

Cited by 46 publications

(17 citation statements)

References 16 publications

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“…The methods used for burning and infecting mice were based on those described by Jones, Jackson and Lowbury (1966). Male hairless mice were used throughout.…”

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confidence: 99%

Treatment of Pseudomonas aeruginosa infections with pyocines

Williams¹

1976

Journal of Medical Microbiology

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PYocrNEs are bacteriocines produced by strains of Pseudomonas aeruginosa and inhibitory to other strains of the same species. They may be classified in two main groups on the basis of their molecular weight. The high molecularweight group (mol. wt. 106-107) is composed of two types of particle: the contractile and the filamentous, both visible in the electron microscope and apparently similar to certain defective bacteriophages. The structure and activity of these pyocines have been described in detail by several workers (Kageyama, 1964;Higerd, Baechler and Berk, 1967;Takeya et al., 1969; Govan, 1974a and b). The other major group of pyocines consists of substances of lower molecular weight (c. lo*), which are not sedimentable by ultracentrifugation and have not been resolved in the electron microscope, but are apparently simple proteins resembling some colicines (Ito, Kageyama and Egami, 1970;Ohkawa, Kageyama and Egami, 1973). These pyocines are known as S type or small. P. aeruginosa poses considerable problems in hospitals because it readilyattacks debilitated patients and, because of its resistance to many antibiotics, treatment of infections is difficult. The specific inhibitory activity of pyocines is well documented and recently the therapeutic use of these agents has been suggested.Bird and Grieble (1969) injected an infective dose of P. aeruginosa into chick embryos, followed immediately by a suitable pyocine preparation, and found that a single dose of pyocine increased the survival of infected embryos from 3 % to 46 %. Similarly, Merrikin and Terry (1972) recorded an improvement in survival of mice infected with P. aeruginosa and treated intravenously with pyocine. However, it is difficult to assess the future of pyocine therapy from these reports, because neither group of workers purified the pyocine preparations, nor did they define clearly the type of pyocine used.The aim of this investigation was to examine the therapeutic use of a representative of each of the three types of pyocine in infections caused by a single sensitive strain of P . aeruginosa. MATERIALS AND METHODSBacterial strains. P. aeruginosa strain P14 was kindly supplied by Dr R. J. Jones, Birmingham Accident Hospital, and was used throughout as the pyocine-sensitive indicator and Govan, 1966) and has a distinctive inhibition pattern. The other strains of P. aeruginosawere from the collection in the Bacteriology Department, Edinburgh University Medical School.One-hundred and seventy strains of P. aeruginosa

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“…The methods used for burning and infecting mice were based on those described by Jones, Jackson and Lowbury (1966). Male hairless mice were used throughout.…”

mentioning

confidence: 99%

Treatment of Pseudomonas aeruginosa infections with pyocines

Williams¹

1976

Journal of Medical Microbiology

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“…Burned mice were completely protected by antiserum and by certain antibiotics (gentamicin and carbenicillin) against fatal invasive infection with Ps. aeruginosa (Jones, Jackson & Lowbury, 1966;Feller, 1966). Because of the risks of anaphylaxis with animal sera and the difficulty of obtaining human immune serum, passive immunization seems impracticable (though it has been used with some apparent success) (Jones & Lowbury, 1967).…”

Section: Pathogenicitymentioning

confidence: 99%

Infection associated with burns

Lowbury

1972

Postgraduate Medical Journal

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“…of a saline suspension of Ps. aeruginosa containing 700 x 106 organisms, spread over the surface of the burn as described by Jones, Jackson & Lowbury (1966). Half of each group of immunized mice was challenged with the same strain of Ps.…”

Section: Protection Testsmentioning

confidence: 99%

Detoxification of an immunogenic fraction from a culture filtrate of Pseudomonas aeruginosa

Jones

1969

J. Hyg.

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SUMMARYMice immunized with a fraction from a culture filtrate of Ps. aeruginosa, then burned and infected with virulent serotypes of Ps. aeroginosa, were protected from septicaemia and death.The immunogenic fraction killed mice at dosages 400 times those of the minimum protective dosages.Treatment of the immunogenic fraction with 0·5% formalin reduced its lethal effect for mice and slightly improved its immunizing potency so that the difference between the smallest immunizing dose used and the smallest amount that killed mice was more than 80,000 doses.

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Antiserum and antibiotic in the prophylaxis of burns against pseudomonas aeruginosa

Cited by 46 publications

References 16 publications

Treatment of Pseudomonas aeruginosa infections with pyocines

Treatment of Pseudomonas aeruginosa infections with pyocines

Infection associated with burns

Detoxification of an immunogenic fraction from a culture filtrate of Pseudomonas aeruginosa

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