Antiserum Against Leukemia Cell Ferritin as a Diagnostic Tool for Malignant Neoplasms2

Abstract

The specific antiserum against a type of ferritin that is especially common to leukemia cells and the placenta was used to test, by countercurrent immunoelectrophoresis, sera from humans with various diseases. The best results were obtained with leukemia; patients with chronic myelogenous leukemia in blastic phase, acute myelogenous leukemia, lymphogenous leukemia, and unclassifiable juvenile leukemia frequently showed a positive reaction, but patients with chronic myelogenous leukemia in static phase did not. The average incidence of positive reaction among all leukemia patients was 54.0%. Patients with other malignant tumors (i.e., multiple myeloma, malignant lymphoma and carcinomas of the stomach, rectum, and liver) also often showed a positive reaction. The average incidence of positive reaction among all the patients with malignant diseases of the hematopoietic system, except for leukemia, was 34.3%, and that among patients with nonhematologic malignant neoplasms was 36.8%. However, the incidence of a positive reaction in patients with benign diseases and healthy individuals was less than 3%.

A biochemical comparison of normal human liver and hepatocellular carcinoma ferritins

“…Increased production of ferritin by tumour cells has been shown in acute leukaemia (White et al, 1974;Mori et al, 1975), but we found low concentrations of ferritin in hepatocellular carcinoma in comparison with normal liver. However, significant elevations of serum ferritin concentrations have been found in patients with liver tumour (Buffe et al, 1970;Alpert et al, 1973;Niitsu et al, 1975).…”

The analysis of N-glycolylneuraminic acid(NeuGc) of hepatoma tissue and K562 cell ferritins using HPLC and mass spectrometry

“…Therefore, its serum level has been noticed as the signal which shows the pathology of those diseases, and especially as a tumor marker. 1) It is also known that ferritin in the malignant tumor tissue shows more acidic property. 2) Lee & Richter 3) supposed that the acidic isoferritin differs from the normal ferritin in amino acid composition.…”

Συμβολη Στη Διαγνωστικη Αξια Της Φερριτινης Του Ορου Σε Διαταραχες Της Οικονομιας Του Σιδηρου Στον Οργανισμο

“…Τό αντιγόνο αυτό έχει απομονωθεί καί καθαριστεί, είναι δέ όμοιο μέ τή φυσιολογική φερριτίνη τοϋ ήπατος καί τοϋ σπλήνα (56). Στή νόσο αυτή ή υψηλή τιμή της φερριτίνης στον όρό (2.500 ng/ml) μπορεί νά έχει μεγάλη σημασία τόσο γιά τή διάγνωση δσο καί γιά τή πρόγνωση της (96,103,186,194).Στους άρρωστους αυτούς έχουν βρεθεί λεμφοκύτ ταρα πού έχουν φερριτίνη στην επιφάνεια τους (149,150).…”