Antisense strategies: functions and applications in immunology

Varga, L; Tóth, Sára; Novak, Istvan; Falus, András

doi:10.1016/s0165-2478(99)00082-6

Cited by 18 publications

(5 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the middle and lower panels of Figure 1, 2 clones (AS1 and AS2) exhibited a significant reduction of the signal corresponding to a protein band around 120 kDa, which matches the molecular weight of MAN 6A8. Since MAN 6A8 catalyses the hydrolysis of p ‐nitro‐phenyl‐α‐D‐mannopyranoside,9 the enzymatic activity of cell homogenate on this substrate was determined. A reduction was observed with homogenates of AS1 and AS2 cells.…”

Section: Resultsmentioning

confidence: 99%

“…Our study was carried out on a human nasopharyngeal carcinoma cell line, CNE‐2L2. Because the chemicals mentioned above cannot specifically suppress a given α‐mannosidase and usually have side effects1 and particularly because swainsonine has no effect on MAN 6A8,8 we used the antisense technique9 to inhibit mRNA translation of MAN 6A8. It was found that the malignant behaviors of CNE‐2L2 cells, both in vitro and in vivo , were significantly reduced when MAN 6A8 expression was inhibited.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Suppression of 6A8 α‐mannosidase gene expression reduced the potentiality of growth and metastasis of human nasopharyngeal carcinoma

Yue

Jin

Shi

et al. 2003

Intl Journal of Cancer

View full text Add to dashboard Cite

Suppression of ␣-mannosidases by chemicals has been shown to reduce the potentiality of growth and metastasis of various tumors. In our study, the effect of 6A8 ␣-mannosidase (MAN 6A8), recently discovered in our laboratory, on malignant behaviors of tumor cells was examined. Since the suppressive effect of chemicals on ␣-mannosidase is not specific, antisense technique was used to specifically inhibit expression of the MAN 6A8 in human nasopharyngeal carcinoma cells, CNE-2L2. Key words: ␣-mannosidase; tumor; growth; metastasis; antisense; E-cadherinInhibition of ␣-mannosidase has been found to show suppressive effect on malignant behaviors of tumor cells. [1][2][3] In examining the effect of ␣-mannosidase on growth and metastasis of tumors, chemicals, such as swainsonine, 1-deoxymannojirimycin, mannostatin, etc., have been normally used to inhibit ␣-mannosidase. 1,3-5 Among the chemicals, swainsonine is the one rather widely used that has been tested even in preliminary clinical trials in late-stage cancer patients and was shown to induce partial remission of tumor in 1 and symptomatic improvement in 2 of 19 patients. 6,7 Recently, we isolated a cDNA encoding a novel human ␣-mannosidase (MAN 6A8). 8 In searching the EST database of the Cancer Genome Anatomy Project for 6A8 gene expression in various tissues, we found an enhancement of 6A8 gene expression in a number of cancer tissues, including bone, bone marrow, brain, esophagus, eye, genitourinary, germ cell, nervous, pineal gland, soft tissue cancers, etc., suggesting a possible role of MAN 6A8 in tumorigenesis. Hence, the relationship between MAN 6A8 gene expression and malignant behaviors of tumor cells has been critically examined. Our study was carried out on a human nasopharyngeal carcinoma cell line, CNE-2L2. Because the chemicals mentioned above cannot specifically suppress a given ␣-mannosidase and usually have side effects 1 and particularly because swainsonine has no effect on MAN 6A8, 8 we used the antisense technique 9 to inhibit mRNA translation of MAN 6A8. It was found that the malignant behaviors of CNE-2L2 cells, both in vitro and in vivo, were significantly reduced when MAN 6A8 expression was inhibited. Since morphologic observation showed that cultured CNE-2L2 cells with inhibited MAN 6A8 expression became compact sheets instead of being dispersed as compared to the controls and since cell-cell adhesion of epithelial cells is mainly mediated by E-cadherin 10 and loss of E-cadherin expression plays an important role in the growth and metastasis of tumors, 11-16 expression of E-cadherin was assayed in CNE-2L2 cells. An enhancement of E-cadherin expression was found in cells with inhibited MAN 6A8 expression.The results demonstrate a significant inhibitive effect of reduced expression of MAN6A8 on the malignant behaviors of CNE-2L2 cells and suggest the possibility of the enzyme being a target molecule in the treatment of nasopharyngeal carcinoma. MATERIAL AND METHODS Cell line and animalCNE-2L2 is a cloned cell line isolated from CNE-2Z, a h...

show abstract

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Suppression of 6A8 α‐mannosidase gene expression reduced the potentiality of growth and metastasis of human nasopharyngeal carcinoma

Yue

Jin

Shi

et al. 2003

Intl Journal of Cancer

View full text Add to dashboard Cite

show abstract

“…Antisense ODN constitutes a potentially important family of therapeutic compounds for the treatment of a range of diseases. 19 In our study, we used antisense ODN strategy to inhibit the NF-kB pathway. Antisense technologies have been used both in vitro and in vivo to inhibit expression of NF-kB proteins in a variety of experimental systems.…”

Section: Discussionmentioning

confidence: 99%

Alleviation of neuropathic pain by intrathecal injection of antisense oligonucleotides to p65 subunit of NF-κB

Sun

Song

et al. 2006

British Journal of Anaesthesia

View full text Add to dashboard Cite

show abstract

“…When a pathogen invades the body, it interacts with specific surface receptors on T and B lymphocytes, causing their activation and production of effector T cells and antibodies that neutralize the pathogen. Many researchers have reported dangerous immune responses with AS ODNs [72]. When phosphorothioates were administered to monkeys as a large, one-time injection, they triggered a systemic and lethal inflammation by activating complement.…”

Section: Immunological Barriermentioning

confidence: 99%