Antisense Rna

Eguchi, Yawara; Itoh, Tateo; Tomizawa, Jun-ichi

doi:10.1146/annurev.bi.60.070191.003215

Cited by 277 publications

(122 citation statements)

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“…PriB has also been shown to be involved in ColE1-type plasmid replication (23). ColE1-type plasmid replication is regulated by interacting RNA primer (RNA II) with its antisense RNA (RNA I) (53). Interestingly, we found that PriB can bind both RNAI and RNAII (Fig.…”

Section: And B)mentioning

confidence: 84%

Crystal Structure of PriB, a Primosomal DNA Replication Protein of Escherichia coli

Liu

Chang²,

Huang

et al. 2004

Journal of Biological Chemistry

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PriB is one of the Escherichia coli X-type primosome proteins that are required for assembly of the primosome, a mobile multi-enzyme complex responsible for the initiation of DNA replication. Here we report the crystal structure of the E. coli PriB at 2.1 Å resolution by multi-wavelength anomalous diffraction using a mercury derivative. The polypeptide chain of PriB is structurally similar to that of single-stranded DNA-binding protein (SSB). However, the biological unit of PriB is a dimer, not a homotetramer like SSB. Electrophoretic mobility shift assays demonstrated that PriB binds single-stranded DNA and single-stranded RNA with comparable affinity. We also show that PriB binds singlestranded DNA with certain base preferences. Based on the PriB structural information and biochemical studies, we propose that the potential tetramer formation surface and several other regions of PriB may participate in protein-protein interaction during DNA replication. These findings may illuminate the role of PriB in X-type primosome assembly.

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Section: And B)mentioning

confidence: 84%

Crystal Structure of PriB, a Primosomal DNA Replication Protein of Escherichia coli

Liu

Chang²,

Huang

et al. 2004

Journal of Biological Chemistry

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“…76 In addition, antisense inhibition is being used to produce cantaloupe plants with delayed fruit ripening and extended self-have been studied most extensively in bacterial systems. 80 Although these RNAs are generally ''highly structured'' and life. 77 Important clinical applications of antisense RNA can be expected in the near future.…”

Section: How Do ''Antisense'' Rnas Regulate Gene Expression?mentioning

confidence: 99%

A hitchhiker's guide to antisense and nonantisense biochemical pathways

Branch

1996

Hepatology

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Antisense pharmaceutical research has sought to provide drugs that would yield effective therapies for disAntisense pharmaceutical research has sought to strategieases resulting from the production of deleterious pro-cally design drugs that would predictably yield safe and effecteins. The original concept was straightforward: tive therapies. It is now clear that a broad knowledge of nueliminate production of unwanted proteins, such as on-cleic acid biochemistry will be needed to attain this goal and cogenic proteins, by blocking the function of their other related objectives. As originally conceived, antisense mRNAs; and block their mRNAs by adding ''antisense'' strategies were to offer a general and rational approach for nucleic acids that bind them through complementary designing treatments for all diseases resulting from the probase pairing. However, it has proven difficult to develop duction of deleterious proteins, such as viral, oncogenic, cytoclinically useful antisense strategies. Conventional anti-toxic, misfolded, or overly abundant proteins (including pepsense nucleic acids are large, highly charged, complex tide hormones). Figure 1 illustrates one type of antisense molecules that interact with a wide variety of unin-approach. In this model, the therapeutic molecule is an antitended cellular and microbial components, often caus-sense DNA oligonucleotide. It is composed of sequences coming ''nonantisense effects.'' It is now clear that a broad plementary to its target, a messenger (mRNA). The mRNA knowledge of nucleic acid biochemistry will be needed contains genetic information in the functional, or sense, orito optimize antisense molecules for use in patients. The entation. In the ideal strategy, binding of the antisense oligoefficacy of naturally occurring antisense molecules and nucleotide inactivates the intended mRNA and prevents its the success of antisense agricultural strategies prove translation into protein, while leaving all other RNAs unafthat antisense approaches can be powerful and specific. fected. 5 Antisense strategies are based on biochemical experiPharmaceutical antisense research can be expected to ments showing 1) that proteins are translated from specific yield many valuable products once sufficient informa-RNAs; 2) that once the sequence of an RNA is known, it is tion about antisense mechanisms has been gathered and possible to design an antisense molecule that will bind to it applied. This article explains the biochemical events through complementary Watson-Crick base pairs; and 3) that that give rise to both antisense and nonantisense effects by manipulating conditions in vitro, it is possible to obtain and provides guidelines for designing and evaluating sequence-specific binding, albeit under conditions that are antisense experiments. (HEPATOLOGY 1996;24:1517-1529.) often far outside the physiological range.Several factors make it difficult to develop clinical applications of the classical antisense model presented in Fig. 1. PREAMBLE

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“…Antisense RNAs bind to the complementary regions (or "sense" strands) of their target RNAs and exert a variety of regulatory functions (1,2). Some of them are encoded in the autonomous replication regions of plasmids and negatively control their replication and copy number (3).…”

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confidence: 99%

“…These antisense RNAs, when expressed in a bacterial cell, repress the replication of identical or closely related plasmids introduced by means of transfer or transformation. This phenomenon is called incompatibility (Inc) 1 and is used to classify plasmids: i.e. if two different plasmids are incompatible in a single bacterial cell, they belong to the same Inc group (4).…”

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confidence: 99%