Antisense RNA directed to the human papillomavirus type 16 E7 mRNA from herpes simplex virus type 1 derived vectors is expressed in CaSki cells and downregulates E7 mRNA

Kari, Ilkka; Syrjänen, Stina; Johansson, Bo; Peri, Piritta; He, Bin; Roizman, Bernard; Hukkanen, Veijo

doi:10.1186/1743-422x-4-47

Cited by 5 publications

(3 citation statements)

References 41 publications

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“…More recently, non-neuroinvasive HSV-1 vectors, lacking the γ 1 34.5 gene, were used to express antisense RNA complementary to the first 100nt of the HPV-16 E7 gene. These recombinant viruses down-regulated E7 protein expression in CaSki cells in a dose-dependent manner (Kari et al, 2007). Overall, these results confirmed the validity of targeting high-risk HPV E6/E7 for cervical cancer therapy.…”

Section: Antisense Technology On Cervical Cancer Therapysupporting

confidence: 69%

Oligonucleotide Applications for the Therapy and Diagnosis of Human Papillomavirus Infection

Benítez‐Hess¹,

Toscano-Garibay²,

Álvarez-Salas³

2012

Human Papillomavirus and Related Diseases - From Bench to Bedside - Research Aspects

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Section: Antisense Technology On Cervical Cancer Therapysupporting

confidence: 69%

Oligonucleotide Applications for the Therapy and Diagnosis of Human Papillomavirus Infection

Benítez‐Hess¹,

Toscano-Garibay²,

Álvarez-Salas³

2012

Human Papillomavirus and Related Diseases - From Bench to Bedside - Research Aspects

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“…Autophagy-associated genes (Beclin1 and p62) are dysregulated in hsa_circ_0023404 depleted and overexpressed in HeLa cells. [69] Several studies have evaluated a treatment strategy using ribozyme (APOBEC) and antisense oligonucleotides to inhibit HPV E6 and E7 mRNA expression [70,71]. However, these approaches have low efficiency, short time of stability, and high cost of design and administration.…”

Section: ] Mir-21 Ptenmentioning

confidence: 99%

The Autophagy Process in Cervical Carcinogenesis: Role of Non-Coding-RNAs, Molecular Mechanisms, and Therapeutic Targets

Lagunas‐Martínez

Madrid‐Marina

Gómez-Cerón

et al. 2022

Cells

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Autophagy is a highly conserved multistep lysosomal degradation process in which cellular components are localized to autophagosomes, which subsequently fuse with lysosomes to degrade the sequestered contents. Autophagy serves to maintain cellular homeostasis. There is a close relationship between autophagy and tumor progression, which provides opportunities for the development of anticancer therapeutics that target the autophagy pathway. In this review, we analyze the effects of human papillomavirus (HPV) E5, E6, and E7 oncoproteins on autophagy processes in cervical cancer development. Inhibition of the expression or the activity of E5, E6, and E7 can induce autophagy in cells expressing HPV oncogenes. Thus, E5, E6, and E7 oncoproteins target autophagy during HPV-associated carcinogenesis. Furthermore, noncoding RNA (ncRNA) expression profiling in cervical cancer has allowed the identification of autophagy-related ncRNAs associated with HPV. Autophagy-related genes are essential drivers of autophagy and are regulated by ncRNAs. We review the existing evidence regarding the role of autophagy-related proteins, the function of HPV E5, E6, and E7 oncoproteins, and the effects of noncoding RNA on autophagy regulation in the setting of cervical carcinogenesis. By characterizing the mechanisms behind the dysregulation of these critical factors and their impact on host cell autophagy, we advance understanding of the relationship between autophagy and progression from HPV infection to cervical cancer, and highlight pathways that can be targeted in preventive and therapeutic strategies against cervical cancer.

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“…However, these early approaches required large quantities of ODNs. Alternatively, different expression vector constructs were employed using plasmids (106)(107)(108), adeno- (109,110) and retroviral vectors (111,112). This considerably improved duration of antisense DNA/RNA mediated silencing and helped in avoiding repeat applications in in vivo settings.…”

Section: Silencing Hpv Rna By Antisense Oligonucleotides (As-odn)mentioning

confidence: 99%

Therapeutic startegies for human papillomavirus infection and associated cancers

et al. 2018

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Human papillomavirus (HPV) infection is linked to development of cancer of cervix, vagina, vulva, penis, ano-genital and non-genital oro-pharyngeal sites. HPV being a sexually transmitted virus infects both genders equally but with higher chances of pathological outcome in women. In the absence of organized screening programs, women report HPV-infected lesions at relatively advanced stages where they are subjected to standard treatments that are not HPV-specific. HPV infection-driven lesions usually take 10-20 years for malignant progression and are preceded by well-characterized pre-cancer stages. Despite availability of window for pharmacological intervention, therapeutic that could eradicate HPV from infected lesions is currently lacking. A variety of experimental approaches have been made to address this lacuna and there has been significant progress in a number of lead molecules which are in different stages of clinical and pre-clinical development. Present review provides a brief overview of the magnitude of the problem and current status of research on promising lead molecules, formulations and therapeutic strategies that showed potential to translate to clinically-viable HPV therapeutics to counteract this reproductive health challenge.

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Antisense RNA directed to the human papillomavirus type 16 E7 mRNA from herpes simplex virus type 1 derived vectors is expressed in CaSki cells and downregulates E7 mRNA

Cited by 5 publications

References 41 publications

Oligonucleotide Applications for the Therapy and Diagnosis of Human Papillomavirus Infection

Oligonucleotide Applications for the Therapy and Diagnosis of Human Papillomavirus Infection

The Autophagy Process in Cervical Carcinogenesis: Role of Non-Coding-RNAs, Molecular Mechanisms, and Therapeutic Targets

Therapeutic startegies for human papillomavirus infection and associated cancers

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