Antisense oligonucleotide technology as a research tool in plant biology

Wdowikowska, Anna; Janicka, Małgorzata

doi:10.1071/fp21194

Cited by 6 publications

(5 citation statements)

References 58 publications

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“…In fact, the application of ASOs in plants is only slowly gaining interest. Mostly used to study gene function, the recent development of methods to introduce ASOs into plants, e.g., by infiltration, has opened up further applications ( [46][47][48][49]; reviewed in [50,51]). Apparently, both types of RNases H (H1 and H2) are present in planta [35], which was confirmed here with the BYL system (Figure 2).…”

Section: Discussionmentioning

confidence: 99%

Effective Antiviral Application of Antisense in Plants by Exploiting Accessible Sites in the Target RNA

Gruber,

Gursinsky,

Gago-Zachert

et al. 2023

IJMS

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Antisense oligodeoxynucleotides (ASOs) have long been used to selectively inhibit or modulate gene expression at the RNA level, and some ASOs are approved for clinical use. However, the practicability of antisense technologies remains limited by the difficulty of reliably predicting the sites accessible to ASOs in complex folded RNAs. Recently, we applied a plant-based method that reproduces RNA-induced RNA silencing in vitro to reliably identify sites in target RNAs that are accessible to small interfering RNA (siRNA)-guided Argonaute endonucleases. Here, we show that this method is also suitable for identifying ASOs that are effective in DNA-induced RNA silencing by RNases H. We show that ASOs identified in this way that target a viral genome are comparably effective in protecting plants from infection as siRNAs with the corresponding sequence. The antiviral activity of the ASOs could be further enhanced by chemical modification. This led to two important conclusions: siRNAs and ASOs that can effectively knock down complex RNA molecules can be identified using the same approach, and ASOs optimized in this way could find application in crop protection. The technology developed here could be useful not only for effective RNA silencing in plants but also in other organisms.

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Section: Discussionmentioning

confidence: 99%

Effective Antiviral Application of Antisense in Plants by Exploiting Accessible Sites in the Target RNA

Gruber,

Gursinsky,

Gago-Zachert

et al. 2023

IJMS

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“…The third mechanism of action relies on the ability of antisense oligonucleotides to form a triple helix by binding to DNA strands, which leads to inhibition of transcription initiation. The fourth mechanism is associated with the formation of the antisense oligonucleotide–mRNA complex, which can affect splicing [ 12 ]. Due to the flexibility of the approach, antisense oligonucleotides can be used against different SARS coronaviruses that are always ‘sweeping their tracks’ during microevolution [ 13 , 14 ].…”

Section: Antisense Antiviralsmentioning

confidence: 99%

DNA Oligonucleotides as Antivirals and Vaccine Constituents against SARS Coronaviruses: A Prospective Tool for Immune System Tuning

Oberemok

Andreeva

Alieva

2023

IJMS

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The SARS-CoV-2 pandemic has demonstrated the need to create highly effective antivirals and vaccines against various RNA viruses, including SARS coronaviruses. This paper provides a short review of innovative strategies in the development of antivirals and vaccines against SARS coronaviruses, with a focus on antisense antivirals, oligonucleotide adjuvants in vaccines, and oligonucleotide vaccines. Well-developed viral genomic databases create new opportunities for the development of innovative vaccines and antivirals using a post-genomic platform. The most effective vaccines against SARS coronaviruses are those able to form highly effective memory cells for both humoral and cellular immunity. The most effective antivirals need to efficiently stop viral replication without side effects. Oligonucleotide antivirals and vaccines can resist the rapidly changing genomic sequences of SARS coronaviruses using conserved regions of their genomes to generate a long-term immune response. Oligonucleotides have been used as excellent adjuvants for decades, and increasing data show that oligonucleotides could serve as antisense antivirals and antigens in vaccine formulations, becoming a prospective tool for immune system tuning.

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“…Currently, two drugs, ISIS Fomivirsen (ISIS 2922) and GEM 132, are undergoing clinical trials for treating HCMV infection. Fomivirsen is a synthetic 21nucleotide phosphorothioate oligodeoxynucleotide that is designed to be complementary to the sequence in HCMV mRNAs that encodes major immediate early 2 proteins [84,85]. These proteins are crucial for HCMV replication, and thus Fomivirsen is designed to inhibit viral replication by blocking the expression of these proteins.…”

Section: Human Cytomegalovirus (Hcmv)mentioning

confidence: 99%

Investigating the Frontiers of Genetic Regulation and Unlocking the Secrets of Gene Expression: The Power of RNA-based Therapeutics

HR¹

2023

J Clin Genet Hered.

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Advancements in genome editing have revolutionized the field of biotechnology by enabling precise manipulation of DNA sequences. The ability to edit genes has significant implications for treating genetic diseases and developing novel therapeutics. Despite the considerable progress made in genome editing, there are still concerns over its off-target effects and ethical considerations, which have spurred the exploration of alternative approaches. In this context, we present a paper on antisense technology, an innovative biological approach that has the potential to regulate gene expression by halting the translation process of mRNA and suppressing protein production. We highlight the limitations of current genome editing technologies and the need for more targeted and personalized approaches to treat genetic disorders. Antisense technology employs artificially synthesized oligonucleotides or small RNA sequences to target specific genes and inhibit their expression. This approach provides greater specificity and control over gene expression, making it a highly promising therapeutic option for various diseases such as cancer, cardiovascular diseases, and viral infections. We discuss the current status of antisense technology, its potential future prospects, as well as the challenges and opportunities that need to be addressed to fully exploit its potential. Overall, our paper aims to shed light on the significance of antisense technology in the field of biotechnology and its potential to revolutionize gene expression regulation. By providing an overview of this innovative approach, we hope to inspire further research in this area and pave the way for novel and more effective therapies for genetic disorders.

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Antisense oligonucleotide technology as a research tool in plant biology

Cited by 6 publications

References 58 publications

Effective Antiviral Application of Antisense in Plants by Exploiting Accessible Sites in the Target RNA

Effective Antiviral Application of Antisense in Plants by Exploiting Accessible Sites in the Target RNA

DNA Oligonucleotides as Antivirals and Vaccine Constituents against SARS Coronaviruses: A Prospective Tool for Immune System Tuning

Investigating the Frontiers of Genetic Regulation and Unlocking the Secrets of Gene Expression: The Power of RNA-based Therapeutics

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