Antisense Expression of Polyphenol Oxidase Genes Inhibits Enzymatic Browning in Potato Tubers

Bachem, Christian W.B.; Speckmann, G. J.; Linde, P. C. G. van der; Verheggen, F.T.M.; Hunt, M. L.; Steffens, John C.; Zabeau, Marc

doi:10.1038/nbt1194-1101

Cited by 132 publications

(74 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Unluckily, there was again no frying colour data available on all 221 genotypes, only 145 genotypes were represented in the phenotypic dataset. POT candidate genes 8 (PPO-III, 25 cM) Bachem et al (1994) 1 3…”

Section: Illustration For Two Marker Trait Associationsmentioning

confidence: 99%

Association mapping of quality traits in potato (Solanum tuberosum L.)

D’hoop¹,

Paulo²,

Mank

et al. 2007

Euphytica

102

View full text Add to dashboard Cite

In this paper, we describe the assessment of linkage disequilibrium and its decay in a collection of potato cultivars. In addition, we report on a simple regression based association mapping approach and its results to quality traits in potato. We selected 221 tetraploid potato cultivars and progenitor lines, representing the global diversity in potato, with emphasis on genetic variation for agro-morphological and quality traits. Phenotypic data for these agro-morphological and quality traits were obtained from recent trials performed by Wve breeding companies. The collection was genotyped with 250 AFLP ® markers from Wve primer combinations. The genetic position of a subset of the markers could be inferred from an ultra dense potato map. Decay of linkage disequilibrium was estimated by calculating the squared correlation between pairs of markers using marker band intensities. Marker-trait associations were investigated by Wtting single marker regression models for phenotypic traits on marker band intensities with and without correction for population structure. The paper illustrates the potential of association mapping in tetraploid potato, because existing phenotypic data, a modest number of AFLP markers, and a relatively simple statistical analysis, allowed identifying interesting associations.

show abstract

Section: Illustration For Two Marker Trait Associationsmentioning

confidence: 99%

Association mapping of quality traits in potato (Solanum tuberosum L.)

D’hoop¹,

Paulo²,

Mank

et al. 2007

Euphytica

102

View full text Add to dashboard Cite

show abstract

“…Therefore, strategies to reduce acrylamide forming potential were the silencing of the acid invertase gene [43] and asparagine synthase-1 (StAst1) gene [35], through intragenic modifications [36,37,44]. Black spot bruise, successfully prevented by silencing polyphenol oxidase (PPO) genes [45], was also obtained by intragenesis, without inserting any foreign DNA in the plant genome. The first generation of Innate™ Simplot potato combined low acrylamide potential (low asparagine) and black spot bruise resistance.…”

Section: Not All Gm Potatoes Are Equalmentioning

confidence: 99%

Effect of Organic Potato Farming on Human and Environmental Health and Benefits from New Plant Breeding Techniques. Is It Only a Matter of Public Acceptance?

Pacifico

Paris

2016

Sustainability

View full text Add to dashboard Cite

Organic farming practices are commonly thought to reduce the environmental impact of agriculture and to preserve the naturalness of the products. Herein, we report the effect of crop management practices on nutritional and toxicological value of potato tubers. Comparative studies are often controversial and the results are dependent on genotype and methodological approach. Targeted analysis and "omics" strategies are discussed, pointing at the nutritional aspects and the corresponding biological and molecular processes involved. Organic farming supporters still do not accept the use of genetic modification to produce new varieties suited for organic agriculture and crop improvement by genetic engineering still sparks hot debate among various scientific and social factions whose major concern is the possible existence of unintended effects both on human and world health. In this context, the advent of "new plant breeding techniques" has reignited the discussion on genetic engineering and on the compatibility of the new technologies with an eco-friendly agriculture. Could cisgenic and genome-edited potatoes be new good options for organic agriculture? We discuss how these approaches can be used to address food security challenges and to overcome specific problems based on the biological characteristics of potato tubers, producing new varieties that can improve farmers' profit with a lower impact on public opinion. However, political, ethical, and social fears will probably persist much longer, mainly in Italy, historically a fiercely anti-GM country with a European leadership in organic food production and export.

show abstract

“…By employing an antisense RNA technique Bachem et al (8) blocked PPO gene expression in potatoes and obtained very low PPO activity-containing cultivars. Coetzer et al (9) also reduced the PPO activity in potatoes by using sense or antisense RNA techniques and successfully controlled their enzymatic browning.…”

Section: Introductionmentioning

confidence: 99%

Consistency of Polyphenol Oxidase (PPO) Thermostability in Ripening Apricots (Prunus armeniaca L.): Evidence for the Presence of Thermostable PPO Forming and Destabilizing Mechanisms in Apricots

Yemenicioğlu

Cemeroğlu

2003

J. Agric. Food Chem.

View full text Add to dashboard Cite

Destabilization of thermostable polyphenol oxidase (TS-PPO) during the ripening of peaches has been previously shown (Yemenicioǧ lu, A.; Cemeroǧ lu, B. Tr. J. Agric. For. 1998, 22, 261-265). This work studied the effect of ripening on thermal stability of apricot PPO for three different cultivars. Kabaaş ı cultivar contained thermolabile PPO, whereas TS-PPO appeared in Hacıhaliloǧ lu and Ç ataloǧ lu cultivars. The TS-PPO showed biphasic inactivation curves, and its D and z values between 60 and 90°C varied in the ranges of 357-1.12 min and 11.9-12.7°C, respectively. In Hacıhaliloǧ lu cultivar the TS-PPO was very consistent and existed at all stages of ripening, whereas in Ç ataloǧ lu cultivar it appeared only at the half-ripe stage. The loss of consistent TS-PPO in Hacıhaliloǧ lu apricots after partial purification by acetone precipitation and DEAE-cellulose chromatography suggested the non-covalent nature of its stabilization. The main purified fractions (F1 and F2) showed monophasic inactivation curves with similar thermal inactivation parameters (z F1 ) 10.4°C, z F2 ) 10.1°C). However, their kinetic properties against catechol (K mF1 ) 61 mM, K mF2 ) 122.7 mM) and substrate specificities were considerably different. The results of this study showed the presence of TS-PPO forming and destabilizing mechanisms in apricots. Further studies are needed for the solution of these mechanisms and to develop some new strategies that may be utilized by molecular techniques for a planned production of apricot cultivars provided with heat labile but normal PPO activity.

show abstract

Antisense Expression of Polyphenol Oxidase Genes Inhibits Enzymatic Browning in Potato Tubers

Cited by 132 publications

References 20 publications

Association mapping of quality traits in potato (Solanum tuberosum L.)

Association mapping of quality traits in potato (Solanum tuberosum L.)

Effect of Organic Potato Farming on Human and Environmental Health and Benefits from New Plant Breeding Techniques. Is It Only a Matter of Public Acceptance?

Consistency of Polyphenol Oxidase (PPO) Thermostability in Ripening Apricots (Prunus armeniaca L.): Evidence for the Presence of Thermostable PPO Forming and Destabilizing Mechanisms in Apricots

Contact Info

Product

Resources

About