Antisense Expression of a Rice Sucrose Transporter OsSUT1 in Rice (Oryza sativa L.)

Abstract

We analyzed the function of a rice sucrose transporter, OsSUT1, by using antisense rice. There was no difference between antisense and wild-type plants in carbohydrate content and photosynthetic ability of the flag leaves in the vegetative growth stage, suggesting that OsSUT1 may not play an important role in carbon metabolism, at least in these materials.

Analysis of Sugar Content and Expression of Sucrose Transporter Genes (<i>OsSUTs</i>) in Rice Tissues in Response to a Chilling Temperature

“…has reportedly had effects on the pollen function (e.g., pollen germination) or on the growth and production of filled grain, resulting in decreased grain weight (Hirose et al 2010, Ishimaru et al 2001, Scofield et al 2002. These reports also suggest that the decrease in expression levels of OsSUT1 in rice sheaths at the booting stage observed in our study may affect the development of anthers, including pollens.…”

Genetic Control of Carbon Partitioning in Grasses: Roles of Sucrose Transporters and Tie-dyed Loci in Phloem Loading

“…This was shown by strongly reducing OsSUT1 gene expression by antisense RNA suppression (Ishimaru et al, 2001;Scofield et al, 2002). Antisense lines with almost complete reduction in RNA or protein levels had normal vegetative growth and no alteration in photosynthesis or leaf carbohydrate contents, contrary to what would be expected if OsSUT1 principally functioned in phloem loading.…”

“…Instead, transgenic plants had reduced grain filling and decreased germination, indicating that OsSUT1 plays an important role in transporting Suc to the developing grain and in remobilizing stored carbohydrates during early seedling growth. No visible phenotype was observed in leaves of OsSUT1 antisense lines, potentially due to genetic redundancy (Ishimaru et al, 2001;Aoki et al, 2003) or to utilization of a symplastic phloem-loading pathway (Kaneko et al, 1980). To our knowledge, these two reports are the only publications to date that directly assess the biological function of a grass SUT in vivo.…”

Overexpression of a Maize SPS Gene Improves Yield Characters of Potato under Field Conditions

“…After centrifugation at 12000 × g for 5 min, the pellets were boiled in distilled water for 2 hr and digested with amyloglucosidase for 15 min at 55°C. The starch and sucrose contents were then measured enzymatically according to the method of Ishimaru et al (2001). All enzymes used in these procedures were obtained from Boehringer Mannheim GmbH (Mannheim, Germany).…”