Antisense and Sense CHS cDNA Expressed by a Tapetum-specific Promoter Causes Partial Male Sterility in Transgenic Tobacco

Matsuda, Nobuyuki; Kishitani, Sachie; Toriyama, Kinya

doi:10.5511/plantbiotechnology.14.157

Cited by 3 publications

(4 citation statements)

References 26 publications

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“…To date, genes encoding key enzymes of the flavonoid biosynthesis pathway, phenylalanine ammonia lyase (PAL) and chalcone synthase (CHS), have been reported to be specifically expressed in the tapetum (Shen and Hsu 1992;Hihara et al 1996). In the disrupted tobacco plants of these two genes (PAL and CHS) by tapetum-specific promoter, a decrease of pollen fertility was observed (Matsuda et al 1996;Matsuda et al 1997). Furthermore, flavonols, which were produced from dihydroflavonol by flavonol synthase, were required for func- tional pollen grains (Deboo et al 1995).…”

Section: Hierarchical Clustering Of Cdna Expressed Abundantly In Anthmentioning

confidence: 99%

Identification and molecular characterization of novel anther-specific genes in Oryza sativa L. by using cDNA microarray

et al. 2004

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The complicated genetic pathway regulates the developmental programs of male reproductive organ, anther tissues. To understand these molecular mechanisms, we performed cDNA microarray analyses and in situ hybridization to monitor gene expression patterns during anther development in rice. Microarray analysis of 4,304 cDNA clones revealed that the hybridization signal of 396 cDNA clones (271 non-redundant groups) increased more than six-fold in every stage of the anthers compared with that of leaves. Cluster analysis with the expression data showed that 259 cDNA clones (156 non redundant groups) were specifically or predominantly expressed in anther tissues and were regulated by developmental stagespecific manners in the anther tissues. These co-regulated genes would be important for development of functional anther tissues. Furthermore, we selected several clones for RNA in situ hybridization analysis. From these analyses, we found several novel genes that show temporal and spatial expression patterns during anther development in addition to anther-specific genes reported so far. These results indicate that the genes identified in this experiment are controlled by different programs and are specialized in their developmental and cell types.

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Section: Hierarchical Clustering Of Cdna Expressed Abundantly In Anthmentioning

confidence: 99%

Identification and molecular characterization of novel anther-specific genes in Oryza sativa L. by using cDNA microarray

et al. 2004

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“…The tap1 promoter of snapdragon has been used to direct the rolB gene to increase indole-3-acetic acid content in anthers, which alters anther development (Spena et al 1992). The Osg6B promoter has been used to direct antisense genes for PAL (Matsuda et al 1996), CHS (Matsuda et al 1997), and AOX (Kitashiba et al 1999) and used for the analysis of the roles of each gene in anther development. To date, our knowledge of the anther-specific promoter characterized in transgenic A. thaliana is restricted.…”

Section: Discussionmentioning

confidence: 99%

Comparative study of promoter activity of three anther-specific genes encoding lipid transfer protein, xyloglucan endotransglucosylase/hydrolase and polygalacturonase in transgenic Arabidopsis thaliana

et al. 2002

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Promoter sequences of three anther-specific genes, each of which shows sequence identity to lipid transfer protein (LTP12), xyloglucan endotransglucosylase/hydrolase (XTH3), and polygalacturonase (PGA4), were obtained from Arabidopsis thaliana, fused to the β-glucuronidase (GUS) gene, and then introduced into A. thaliana. Histochemical GUS assay showed that the PGA4 promoter was active in the tapetum at the bicellular pollen stage and in tricellular pollen. The promoter of LTP12 and XTH3 directed GUS expression exclusively in the tapetum. The LTP12 promoter was activated from the uninucleate microspore stage, while the XTH3 promoter was activated from the bicellular pollen stage. This type of activation pattern at the late developmental stage of the tapetum has not been reported previously. The promoter sequences employed in this study will be useful for the characterization of genes differentially expressed in anthers.

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“…In addition, some flavonol-deficient plants are self-sterile because the pollen grains fail to germinate (Mo et al 1992;Taylor and Helper 1997;Taylor and Jorgensen 1992), or fail to produce a functional pollen tube (Pollak et al 1995;Taylor and Helper 1997;Ylstra, 1994). Matsuda et al (1997) produced partial male sterility in transgenic tobacco by sense and antisense suppression of CHS. To induce flavonol deficiency and male sterility in the anthers of gentian plants, GtFLS would be one of the targets for gene silencing, because GtFLS regulates anther-specific accumulation of flavonols in gentian.…”

Section: Gus Expression Of Gtflsprogus-expressing Transgenic Plantsmentioning

confidence: 99%

“…In addition, the deficiency of flavonols in anthers of maize and petunia is known to induce male sterility (Mo et al 1992;Taylor and Helper 1997;Taylor and Jorgensen 1992). Anther-specific suppression of CHS transcripts in transgenic petunia and tobacco also resulted in male sterility (Matsuda et al 1997;van der Meer et al 1992). Therefore, flavonols accumulated in anthers were thought to play an important role in anther and pollen development.…”

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A flavonol synthase gene GtFLS defines anther-specific flavonol accumulation in gentian

Kimura

Nakatsuka

Yamada

et al. 2010

Plant Biotechnology

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Flavonoids are important for male gametophyte development. Here we report on the flavonoid components and their biosynthetic regulation in gentian anthers. Among flavonoids, flavonols, including kaempferol, quercetin and isorhamnetin derivatives, accumulated abundantly in gentian anthers. However, flavones and anthocyanins, which are the main flavonoids accumulating in petals, were not detected. Northern blot analysis of nine flavonoid biosynthetic genes showed that the 'early' flavonoid biosynthetic genes were expressed in both anthers and petals, and that flavonol synthase (FLS) transcripts were restricted to anthers. In contrast, flavone synthase II (FNSII) and 'late' flavonoid biosynthetic genes were expressed specifically in gentian petals. To confirm anther-specific expression of FLS, the 5Ј-upstream region of FLS (GtFLSpro) was cloned by inverse PCR and fused to the uidA (GUS) reporter gene. Tobacco, Arabidopsis and gentian plants, transformed with the GtFLSproGUS construct, exhibited anther-specific GUS expression. Expression was observed in the tapetum and in pollen at late stages of anther development in transgenic plants. These results revealed that flavonol accumulation in gentian anthers was regulated by the spatial expression of GtFLS. Our results also suggest that antherspecific regulation of FLS is conserved among higher plants and the GtFLS promoter is useful for induction of specific gene expression in anthers.Key words: Anther, flavonol synthase, gentian, promoter, transgenic plants. Plant Biotechnology28, 211-221 (2010) DOI: 10.5511/plantbiotechnology.10.1109c Original PaperAbbreviations: ANS, anthocyanidin synthase; CHI, chalcone isomerase; CHS, chalcone synthase; DFR, dihydroflavonol 4-reductase; F3H, flavanone 3-hydroxylase; F3ЈH, flavonoid 3Ј-hydroxylase; F3Ј5ЈH, flavonoid 3Ј,5Ј-hydroxylase; FLS, flavonol synthase; FNSII, flavone synthase II; HPLC, high performance liquid chromatography; RACE, rapid amplification of cDNA ends The nucleotide sequences reported in this paper have been submitted to DDBJ under accession numbers AB587658 and AB587659. † The authors contributed equally to this work. This article can be found at

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Antisense and Sense CHS cDNA Expressed by a Tapetum-specific Promoter Causes Partial Male Sterility in Transgenic Tobacco

Cited by 3 publications

References 26 publications

Identification and molecular characterization of novel anther-specific genes in Oryza sativa L. by using cDNA microarray

Identification and molecular characterization of novel anther-specific genes in Oryza sativa L. by using cDNA microarray

Comparative study of promoter activity of three anther-specific genes encoding lipid transfer protein, xyloglucan endotransglucosylase/hydrolase and polygalacturonase in transgenic Arabidopsis thaliana

A flavonol synthase gene GtFLS defines anther-specific flavonol accumulation in gentian

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