Antirotaviral potential of lactoferrin from different origin: effect of thermal and high pressure treatments

Abstract

Rotaviral gastroenteritis causes a high rate of infant mortality and severe healthcare implications worldwide. Several studies have pointed out that human milk and dairy fractions, such as whey and buttermilk, possess antirotaviral activity. This activity has been mainly associated with glycoproteins, among them lactoferrin (LF). Thermal treatments are necessary to provide microbiological safety and extend the shelf life of milk products, though they may diminish their biological value. High hydrostatic pressure (HHP) treatment is a non-thermal method that causes lower degradation of food components than other treatments. Thus, the main objective of this study was to prove the antirotaviral activity of LFs from different origin and to evaluate the effect of several thermal and HHP treatments on that activity. LF exerted a high antirotaviral activity, regardless of its origin. Native LFs from bovine, ovine, swine and camel milk, and the human recombinant forms, at 1 mg/mL, showed neutralizing values in the range 87.5-98.6%, while human LF neutralized 58.2%. Iron saturation of bovine LF did not modify its antirotaviral activity. Results revealed interspecies differences in LFs heat susceptibility. Thus, pasteurization at 63 °C for 30 min led to a decrease of 60.1, 44.5, 87.1, 3.8 and 8% of neutralizing activity for human, bovine, swine, ovine and camel LFs, respectively. Pasteurization at 75 °C for 20 s was less harmful to the activity of LFs, with losses ranging from 0 to 13.8%. HHP treatment at 600 MPa for 15 min did not cause any significant decrease in the neutralizing activity of LFs.

Properties of Sweet Buttermilk Released from the Churning of Cream Separated from Sheep or Cow Milk or Sheep Cheese Whey: Effect of Heat Treatment and Storage of Cream

“…Parrón et al [38] reported similar protein profile and lactose and protein contents for cow and sheep buttermilk, but higher lipid content for the latter. The same group [38][39][40] reported a higher dosedependent antiviral activity of sheep buttermilk and its components compared with its cow counterpart. The gross composition and cheese making potential of sweet sheep and goat buttermilk alone or in mixtures with milk with variable fat content has been investigated by Sakkas et al [24].…”

Lactoferrin modulates gut microbiota and Toll-like receptors (TLRs) in mice with dysbiosis induced by antibiotics

“…17,20 However, the same antiviral activity against Rotavirus has been observed for bovine Lf, in both native and holo forms. 21 Several assays have also shown that, depending on iron saturation, Lf displays divergent effects on the growth of probiotic bacteria. 17 The capacity of bLf to modulate the gut microbiota positively has been previously demonstrated in high-fat diet induced obese mice, 22 dextran sulfate sodium induced colitis model 23 or Alzheimer's disease models.…”

Preservation of Anti-cytomegalovirus Activity in Human Milk Following High-Pressure Processing Compared to Holder Pasteurization

“…Although we observed no anti-HAV activity, it is possible that other non-enveloped viruses could be partially inactivated by human milk. In fact, previous studies have demonstrated that human milk can partially inactivate rotavirus, a non-enveloped virus; further evidence is needed to examine other non-enveloped viruses ( 39 , 40 ).…”