Antirhea borbonica Aqueous Extract Protects Albumin and Erythrocytes from Glycoxidative Damages

Abstract

Diabetes constitutes a major health problem associated with severe complications. In hyperglycemic conditions, chronically increased oxidation and glycation of circulating components lead to advanced glycation end-products (AGEs) formation, a key contributor in diabetes complication progression. In line with literature documenting the beneficial properties of herbal teas, this study evaluates the antioxidant/glycant properties of Antirhea borbonica (Ab). Ab aqueous extract effects were tested on human albumin or erythrocytes submitted to methyl glyoxal-mediated glycoxidative damages. By using mass spectrometry, Ab aqueous extracts revealed to be rich in polyphenols. All tested biomarkers of oxidation and glycation, such as AGE, ketoamine, oxidized thiol groups, were decreased in albumin when glycated in the presence of Ab aqueous extract. Ab extract preserve erythrocyte from methylglyoxal (MGO)-induced damages in terms of restored membrane deformability, reduced oxidative stress and eryptosis phenomenon. Antioxidant capacities of Ab extract on erythrocytes were retrieved in vivo in zebrafish previously infused with MGO. These results bring new evidences on the deleterious impacts of glycation on albumin and erythrocyte in diabetes. Furthermore, it reveals antioxidant and antiglycant properties of Ab that could be used for the dietary modulation of oxidative stress and glycation in hyperglycemic situations.

Evaluation of Polyphenol Content and Antioxidant Capacity of Aqueous Extracts from Eight Medicinal Plants from Reunion Island: Protection against Oxidative Stress in Red Blood Cells and Preadipocytes

“…It is important to note that PRE exert high protective effects at 25 µM by increasing the HT50 from 8.01 ± 0.16 h (for H. ambavilla ) to 11.72 ± 0.12 h (for D. viscosa ). As previously reported, polyphenols may prevent hemolysis and thus increase red blood cell half-life [ 24 , 26 ]. As presented in Figure 4 , at a final concentration of 25 µM GAE, half-hemolysis time was prolonged from 2.9 ± 0.2 h to 6.9 ± 0.1 h for the infusions, from 2.3 ± 0.2 h to 5.8 ± 0.1 h for the decoctions and from 1.5 ± 0.2 h to 5.2 ± 0.1 h for the PRE.…”

“…Several reports emphasize on the use of medicinal plants and polyphenols to prevent oxidative stress damage notably in red blood cells [ 24 , 51 , 52 , 53 , 54 ]. Oxidative stress affects membrane stability and fluidity [ 55 ].…”

Medicinal Plant Polyphenols Attenuate Oxidative Stress and Improve Inflammatory and Vasoactive Markers in Cerebral Endothelial Cells during Hyperglycemic Condition

“…The most traditionally employed method for preparation of herbal remedies is infusion, which consists of soaking the plant part in very hot water [ 50 ]. Recently, A. borbonica plant infusion was shown to exert antioxidant properties on in vitro and in vivo models exposed to glycoxidative condition [ 90 ]. It will be of interest to evaluate the benefits of infusions that were prepared from the medicinal plants tested here, during cerebrovascular complications related to diabetes.…”

Caffeic Acid, One of the Major Phenolic Acids of the Medicinal Plant Antirhea borbonica, Reduces Renal Tubulointerstitial Fibrosis

“…Antirhea borbonica ( A. borbonica ) leaves are peculiarly interesting, as they are widely used in traditional medicine for treating, among others, diabetes, urinary tract infection, diarrhea, hemorrhage, rheumatism and also kidney stones [ 26 , 27 ]. Our laboratory has shown that A. borbonica exhibited strong antioxidant and anti-inflammatory effects, in vitro, on preadipocytes, cerebral endothelial cells and red blood cells [ 22 , 28 , 29 ]. Those antioxidant and anti-inflammatory biological effects were mainly associated with the capacity of polyphenols to down-regulate on one hand key molecular targets such as IL6, MCP-1 and NF-kB, and on the other hand increase superoxide dismutase (SOD) as well as the redox-sensitive translational factor Nrf2.…”

“…Those antioxidant and anti-inflammatory biological effects were mainly associated with the capacity of polyphenols to down-regulate on one hand key molecular targets such as IL6, MCP-1 and NF-kB, and on the other hand increase superoxide dismutase (SOD) as well as the redox-sensitive translational factor Nrf2. In addition, the in vitro studies showed that predominant polyphenols such as quercetin, chlorogenic and caffeic acids were able to reduce free radicals through DPPH and AAPH radical-scavenging tests [ 22 , 28 , 29 ].…”