Antiradical Activity Study of Momordica charantia L Seeds Based on DPPH and its Secondary Metabolites Analysis

Abstract

Momordica charantia L seed is classified as the domestic waste even though it has many health benefits, namely as anti-tumor, anti-virus, and as a medicine to increase the body resistance. Therefore, this study was designed to examine the antioxidant activity of the ethanol extract of the seeds using the DPPH method. Furthermore, a GC-MS analysis will be carried out to determine the chemical compounds that play a role in the bioactivity. Based on the absorbance of DPPH in three repetitions, the average value of DPPH inhibition by 95% ethanol extract of bitter melon seeds was 60.45%, in which classified as a good radical absorbance. The GC-MS results showed that the ethanol extract of bitter melon seeds contained ten chemical compounds consisting of sesquiterpenes, fatty acids, and alkanes, which affecting the radical absorbance of M. charantia extract.

Ekstrak Jeruk Nipis Sebagai Obat Terapi Penyembuhan Penyakit Gastritis dengan metode GC-MS

“…25µm, suhu injeksi 40 o C selama 5 menit dengan peningkatan suhu sebesar 30 o C/menit selama 7 menit hingga mencapai 260 o C. Fase gerak yang digunakan yaitu gas Helium dengan kecepatan rata-rata 30ml/min. Identifikasi oleh mass spectrum diatur pada 35-500 m/z (Ratnasari et al, 2022). Identifikasi senyawa kemudian dilakukan dengan membandingkan spektrum massa sample dengan database pada system di dalam GC-MS.…”