Antipurified-Protein-Derivative Antibody in Tuberculous Pleural Effusions

Murate, Takanao; Mizoguchi, Kenji; Amano, Hiroshi; Shimokata, Kaoru; Matsuda, Tsukasa

doi:10.1378/chest.97.3.670

Cited by 8 publications

(4 citation statements)

References 13 publications

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“…On the other hand, B lymphocytes produce antibodies against mycobacterial antigens. Immunoassays to detect the presence of antibodies in pleural fluid against purified protein derivative 12, tuberculous glycolipid antigen 13 and lipoarabinomannan 14 in patients with TPE have been documented. Recently, Kaisermann et al detected IgA antibodies against MPT‐64 and MT‐10.3 recombinant mycobacterial antigens in pleural fluids 15.…”

Section: Discussionsupporting

confidence: 78%

Diagnostic significance of humoral immune responses to recombinant antigens of Mycobacterium tuberculosis in patients with pleural tuberculosis

Sumi¹,

Radhakrishnan²

2010

Clinical Laboratory Analysis

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Mycobacterium tuberculosis bacilli are seldom demonstrated in tuberculous pleural effusion (TPE) by conventional bacteriological methods. In this study, an indirect enzyme-linked immunosorbent assay (ELISA) was developed to detect IgG against four mycobacterial recombinant antigens (ESAT-6, PlcA, HspX and Tb8.4) in 69 pleural fluids of patients with TPE and 71 patients with malignant pleural effusion. To increase the sensitivity of the assay, a multi-antigen cocktail containing all the above antigens were also used. IgG positivity in ELISA for PlcA, HspX, Tb8.4, ESAT-6 antigens and multi-antigen complex were 49.3, 60.8, 49.3, 53.6 and 75.4% respectively. Each one of the above four antigens and their multi-antigen cocktail were highly specific in distinguishing tuberculous and malignant pleural effusion. This new generation immunoassay will serve as a useful marker for the diagnosis of pleural tuberculosis patients in whom M. tuberculosis bacilli were not demonstrated by bacteriological methods.

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Section: Discussionsupporting

confidence: 78%

Diagnostic significance of humoral immune responses to recombinant antigens of Mycobacterium tuberculosis in patients with pleural tuberculosis

Sumi¹,

Radhakrishnan²

2010

Clinical Laboratory Analysis

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“…Banchuin et al have reported the absence of significant difference in the IgG levels for PPD in pleural fluid between patients with tuberculosis and malignancy [22] . A contrary report by Murate et al, demonstrated a significant increase of all the three isotypes (IgA, IgG and IgM) for PPD in the tuberculous pleural effusion than the malignant pleurisy [23] . There are very few reports on the pleural antibody response to secretary antigens of mycobacteria.…”

Section: Discussionmentioning

confidence: 68%

Humoral Immune Response in Tuberculous Pleuritis

Prabha¹,

Jalapathy²,

Das³

2005

American J. of Immunology

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Tuberculous pleuritis is a good human model to understand the local and protective immune response against tuberculosis, due to the self-limitedness of the disease. Although the cellular immune response has been well characterised in tuberculous pleurisy, much less is known about the humoral immune response operating at the site of infection. To understand the humoral immune response, B cells were enumerated in peripheral blood mononuclear cells (PBMC) and pleural fluid mononuclear cells (PFMC) of tuberculous (TP) and non-tuberculous pleuritis patients (NTP). The levels of IgG, IgA and IgM antibodies for PPD, culture filtrate (CF) and sonicate antigens (Son Ag) were assessed in plasma (BL) and pleural fluid (PF) and a western blot was carried out with the CF antigen. The percentage of CD19 + B-cells was similar in PBMC and PFMC of TP patients but was significantly lower in PFMCs of NTP patients. The IgG levels for PPD and CF antigens were higher in PF of TP than NTP patients. The antigen recognition patterns did not differ in plasma and pleural fluid of the same patient in both groups pointing out the passive diffusion of the plasma to the pleura. The antigens 25, 31, 33, 70, 110, 124 and 132 kDa were recognized exclusively by the TP patients. Thus our study showed that the local humoral response in TP did not differ from the systemic response. However, the humoral response differed in TP patients when compared to NTP patients.

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“…In this case, a non-vented circuit may be preferred to a vented circuit [34]. However, the ability to compensate for unintentional leaks can be suboptimal with non-vented circuits in patients using volume-guaranteed modes (e.g., volume-assured pressure support), thus leading to decreased inspiratory pressure (or delivered Vt) [35,36]. Therefore, there is a possibility of misinterpretation of unintentional leaks as an increase in Vt [37].…”

Section: Single-limb Circuit With An Exhalation Valve (Ie Non-ventmentioning

confidence: 99%

Home mechanical ventilation: back to basics

Park

Suh

2020

Acute Crit Care

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Home mechanical ventilation: back to basics Over recent decades, the use of home mechanical ventilation (HMV) has steadily increased worldwide, with varying prevalence in different countries. The key indication for HMV is chronic respiratory failure with alveolar hypoventilation (e.g., neuromuscular and chest wall disease, obstructive airway diseases, and obesity-related respiratory failure). Most modern home ventilators are pressure-targeted and have sophisticated modes, alarms, and graphics, thereby facilitating optimization of the ventilator settings. However, different ventilators have different algorithms for tidal volume estimation and leak compensation, and there are also several different circuit configurations. Hence, a basic understanding of the fundamentals of HMV is of paramount importance to healthcare workers taking care of patients with HMV. When choosing a home ventilator, they should take into account many factors, including the current condition and prognosis of the primary disease, the patient's daily performance status, time (hr/day) needed for ventilator support, family support, and financial costs. In this review, to help readers understand the basic concepts of HMV use, we describe the indications for HMV and the factors that influence successful delivery, including interface, circuits, ventilator accessories, and the ventilator itself.

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Antipurified-Protein-Derivative Antibody in Tuberculous Pleural Effusions

Cited by 8 publications

References 13 publications

Diagnostic significance of humoral immune responses to recombinant antigens of Mycobacterium tuberculosis in patients with pleural tuberculosis

Diagnostic significance of humoral immune responses to recombinant antigens of Mycobacterium tuberculosis in patients with pleural tuberculosis

Humoral Immune Response in Tuberculous Pleuritis

Home mechanical ventilation: back to basics

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