1988
DOI: 10.1159/000281351
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Antiproliferative Effects of Interferons against Human Bladder Carcinoma Cell Lines in vitro

Abstract: In order to evaluate the antiproliferative effects of recombinant human interferon-Γ2c (rHu IFN-Γ2c), recombinant human interferon-Γ (rHu IFN-Γ), natural interferon-Β (IFN-Β), and their combination with cytotoxic agents, 17 different human bladder carcinoma cell lines were tested in vitro. The antiproliferative effects were compared in evaluating the tumor cell inhibiting potency of the different interferon (IFN) classes. It could be demonstrated that interferons have inhibiting effects o… Show more

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Cited by 17 publications
(6 citation statements)
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“…Anti-proliferative effects of type I IFN have been reported for diverse cell types, including bladder cancer; however, some of the earlier reports described assays that do not distinguish between cell proliferation arrest and cell death. 13 , 14 , 17 To clarify the effects of type I IFN on proliferation of bladder cancer cells, we chose an assay (5-ethynyl-2′-deoxyuridine [EdU] incorporation) that measures cell-cycle progression rather than cell number, because cell number can be a compound effect of cell proliferation and/or viability. We assembled a panel of 12 diverse human bladder cancer cell lines ( Table 1 ) and the non-transformed immortalized cell line SV-HUC-1, and measured cell-cycle progression from G1 to S phase in response to stimuli.…”
Section: Resultsmentioning
confidence: 99%
“…Anti-proliferative effects of type I IFN have been reported for diverse cell types, including bladder cancer; however, some of the earlier reports described assays that do not distinguish between cell proliferation arrest and cell death. 13 , 14 , 17 To clarify the effects of type I IFN on proliferation of bladder cancer cells, we chose an assay (5-ethynyl-2′-deoxyuridine [EdU] incorporation) that measures cell-cycle progression rather than cell number, because cell number can be a compound effect of cell proliferation and/or viability. We assembled a panel of 12 diverse human bladder cancer cell lines ( Table 1 ) and the non-transformed immortalized cell line SV-HUC-1, and measured cell-cycle progression from G1 to S phase in response to stimuli.…”
Section: Resultsmentioning
confidence: 99%
“…Thus, both CD40L and IFN-b confer negative effects on carcinoma growth by improving tumor cell immunogenicity (4,14,15) and triggering direct antiproliferative and proapoptotic properties (37)(38)(39)(40)(41). Both CD40L and type I IFNs also act on immune cells to augment Th1 cell responses, enhance expression of MHC class I molecules, and generate NK and T cellmediated cytotoxicity (reviewed in Refs.…”
Section: Discussionmentioning
confidence: 99%
“…The bladder carcinoma cell lines EJ and VM-CUB-1 (26) were maintained in RPMI medium supplemented with 10% fetal calf serum (FCS). The cervical cancer line HeLa and the human embryonic kidney (HEK) line 293 were cultured in Dulbecco's modified Eagle medium supplemented with 10% FCS (GIBCO).…”
Section: Methodsmentioning
confidence: 99%