Antiproliferative effects of bioaccessible fractions of honeys from Sicilian black honeybee (Apis mellifera ssp. sicula) on human colorectal carcinoma cells

Abstract

The aim of this study was to evaluate the antiproliferative activity of bioaccessible fractions (BFs) obtained by the internationally standardized INFOGEST static digestion method to Sicilian honeys of three distinct floral origins (Sulla, Thistle and Dill) and the Manuka honey (gold standard), and to compare their effects based on total polyphenol content (TPC). Differentiated CaCo-2 cells (intestinal-like) and nondifferentiated CaCo-2 and HCT-116 colonic tumour-like cells were incubated for 24 h with BFs of honeys to test viability, apoptosis, mitochondrial membrane potential (MMP), ROS and cell cycle. TPC after digestion ranked in the following order: Dill > Thistle > Sulla > Manuka. No decrease in cell viability in differentiated CaCo-2 cells was observed, while a reduction to 25-85% (CaCo-2) and 20-80% (HCT-116) of viability was obtained. This descent in viability was caused by a cell cycle block with an increase in apoptosis through dissipation of MMP and raise in ROS levels, being Thistle and Dill the most effective honeys followed by Sulla and finally Manuka, in agreement with TPC after digestion.

Review: Harmonised in vitro digestion and the Ussing chamber for investigating the effects of polyphenols on intestinal physiology in monogastrics and ruminants

“…2022 Cilla et al 2022 Lettuce (Lactuca sativa L.) Bioaccessibility of polyphenols and antioxidant capacity of fresh or minimally processed modern or traditional lettuce (Lactuca sativa L.) varieties.…”

Determination of hydroxymethylfurfural in Turkish honeys, pekmez (grape molasses), and jam samples byhigh‐performanceliquid chromatography with diode array detection

“…Since honey is a unique product containing natural rich amino acid sources, vitamins, minerals, and biologically active compounds, it has medical value for health (Cilla et al, 2021; Kasiotis & Machera, 2017; Mokaya et al, 2020; Ozsahin & Kirecci, 2018; Perna et al, 2021). Although it has nutritional and medicinal properties, it may also produce some toxicological effects or unwanted compounds that have not been evaluated yet.…”

Bioaccessibility and bioavailability of food‐derived bioactive ingredients and their health‐promoting effects: editorial

“…Honey from the Sicilian black bee, for example is partly biogenic for its anti-proliferative effects (Apis mellifera ssp. sicula) on human colorectal carcinoma cells was illustrated (Cilla et al, 2021). The potential mechanism of curcumin as a-glucosidase inhibitor to prevent diabetes mellitus was also revealed .…”