Antiproliferative effects of Australian native plums against the MCF7 breast adenocarcinoma cells and UPLC-qTOF-IM-MS-driven identification of key metabolites

Dissanayake, Indeewarie Hemamali; Alsherbiny, Muhammad A.; Chang, Dennis; Li, Chun Guang; Bhuyan, Deep Jyoti

doi:10.1016/j.fbio.2023.102864

Cited by 3 publications

(9 citation statements)

References 66 publications

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“…Based on these fragment ions and further comparison with the reference compound, it was identified as ellagic acid. Other phenolic acids were identified by comparison of the accurate molecular weights, fragmentation patterns, and related literature 14–23 …”

Section: Resultsmentioning

confidence: 99%

A comprehensive strategy for quality evaluation of Changan powder by fingerprinting combined with rapid qualitative and quantitative multi‐ingredients profiling

Wang,

Zhang,

et al. 2024

Phytochemical Analysis

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IntroductionChangan powder (CAP) is mainly used to treat various intestinal diseases. Few studies on CAP have been reported; therefore, it is necessary to clarify the material basis of CAP to lay the foundation for further elucidating its functional mechanism and support the rational use of drugs.ObjectivesIn the present study, we aimed to propose a methodology for the quality control of CAP based on qualitative and quantitative analysis of its components.MethodsAn ultra‐performance liquid chromatography coupled with Fourier transform ion cyclotron resonance mass spectrometry (UPLC‐FT‐ICR‐MS) method was developed to identify chemical components in CAP. In addition, fingerprints of 10 different batches of CAP were established, and quantitative analysis based on UPLC was performed to analyze the quality of CAP.ResultsA total of 58 compounds were preliminarily characterized. The similarity of 10 batches of CAP was greater than 0.995, and 23 common peaks were calibrated. Investigation of the quantitative analytical methodology showed that the four components had good linear relationships within their respective concentration ranges (r2 ≥ 0.9992), and the relative standard deviation (RSD) of precision and stability was less than 2%. The RSD of sample recovery ranged from 0.78% to 1.52%.ConclusionThe established method can quickly and effectively identify the chemical components of CAP and accurately quantify the known components in CAP. The established fingerprinting and content determination method is stable, reliable, and easy to operate and can be applied in quality control and in vivo research on CAP.

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Section: Resultsmentioning

confidence: 99%

A comprehensive strategy for quality evaluation of Changan powder by fingerprinting combined with rapid qualitative and quantitative multi‐ingredients profiling

Wang,

Zhang,

et al. 2024

Phytochemical Analysis

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“…The cell viability of the AGS cells after treatment with different concentrations of the three postbiotics (MgA, NaP, NaB) and Dex was determined using the Alamar blue assay as per the method described earlier ( 28 , 29 ). Briefly, 100 μL of cells were cultured in 96 well-plates at a seeding density of 10 5 cells/mL.…”

Section: Methodsmentioning

confidence: 99%

“…The effect of the most active gut metabolites and their most synergistic combinations on the oxidative stress of the cancer cells was studied as per our recently reported protocol using the H2DCFDA (2′,7′-dichlorofluorescein diacetate) cellular reactive oxygen species (ROS) Detection Assay Kit (#ab113851; Abcam, Melbourne, VIC, Australia) ( 19 , 29 ). Briefly, AGS cells (2.5 × 10 5 cells/mL) were cultured in a 96-well plate, adhered overnight, and treated with 20 μM H2DCFDA for 45 min to assess ROS levels.…”

Section: Methodsmentioning

confidence: 99%

“…The impact of the most potent postbiotic and its most synergistic combination with Dex on the apoptosis profiles of the AGS adenocarcinoma cells was studied using an annexin V and 7-AAD-based kit (#ab214663, Abcam, Melbourne, VIC, Australia) ( 19 , 29 ). The AGS cells were cultured in T75 cell culture flasks with an initial density of 1 × 10 6 cells per 10 mL at 37°C in the presence of 5% CO 2 for 24 h. The following day, the cell culture media was removed from each flask and replaced with fresh FBS-containing media, and the cultured flasks were then treated with the highest concentration of the most active postbiotic (3,000 μg/mL), 200 μg/mL of Dex, 0.54 μg/mL (1 μM) of Dox (used as a positive control).…”

Section: Methodsmentioning

confidence: 99%

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The postbiotic sodium butyrate synergizes the antiproliferative effects of dexamethasone against the AGS gastric adenocarcinoma cells

Eladwy,

Alsherbiny,

Chang

et al. 2024

Front. Nutr.

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A growing body of literature underlines the fundamental role of gut microbiota in the occurrence, treatment, and prognosis of cancer. In particular, the activity of gut microbial metabolites (also known as postbiotics) against different cancer types has been recently reported in several studies. However, their in-depth molecular mechanisms of action and potential interactions with standard chemotherapeutic drugs remain to be fully understood. This research investigates the antiproliferative activities of postbiotics- short-chain fatty acid (SCFA) salts, specifically magnesium acetate (MgA), sodium propionate (NaP), and sodium butyrate (NaB), against the AGS gastric adenocarcinoma cells. Furthermore, the potential synergistic interactions between the most active SCFA salt-NaB and the standard drug dexamethasone (Dex) were explored using the combination index model. The molecular mechanisms of the synergy were investigated using reactive oxygen species (ROS), flow cytometry and biochemometric and liquid chromatography-mass spectrometry (LC–MS)-driven proteomics analyses. NaB exhibited the most significant inhibitory effect (p < 0.05) among the tested SCFA salts against the AGS gastric cancer cells. Additionally, Dex and NaB exhibited strong synergy at a 2:8 ratio (40 μg/mL Dex + 2,400 μg/mL NaB) with significantly greater inhibitory activity (p < 0.05) compared to the mono treatments against the AGS gastric cancer cells. MgA and NaP reduced ROS production, while NaB exhibited pro-oxidative properties. Dex displayed antioxidative effects, and the combination of Dex and NaB (2,8) demonstrated a unique pattern, potentially counteracting the pro-oxidative effects of NaB, highlighting an interaction. Dex and NaB individually and in combination (Dex:NaB 40:2400 μg/mL) induced significant changes in cell populations, suggesting a shift toward apoptosis (p < 0.0001). Analysis of dysregulated proteins in the AGS cells treated with the synergistic combination revealed notable downregulation of the oncogene TNS4, suggesting a potential mechanism for the observed antiproliferative effects. These findings propose the potential implementation of NaB as an adjuvant therapy with Dex. Further investigations into additional combination therapies, in-depth studies of the molecular mechanisms, and in vivo research will provide deeper insights into the use of these postbiotics in cancer, particularly in gastric malignancies.

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“…22–24 Our recent study discussed the promising antiproliferative properties of the Kakadu plum, compared to the Illawarra plum and Davidson's plum against MCF7 cells through the induction of apoptosis. 25 Furthermore, the Kakadu plum was found to contain a number of tannins, flavonoids, triterpenoids and other potential bioactive metabolites. 25…”

Section: Introductionmentioning

confidence: 99%

Exploring the broad-spectrum pharmacological activity of two less studied Australian native fruits: chemical characterisation using LCMS-driven metabolomics

Dissanayake,

Alsherbiny,

Stack

et al. 2024

Food Funct.

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Antiproliferative effects of Australian native plums against the MCF7 breast adenocarcinoma cells and UPLC-qTOF-IM-MS-driven identification of key metabolites

Cited by 3 publications

References 66 publications

A comprehensive strategy for quality evaluation of Changan powder by fingerprinting combined with rapid qualitative and quantitative multi‐ingredients profiling

A comprehensive strategy for quality evaluation of Changan powder by fingerprinting combined with rapid qualitative and quantitative multi‐ingredients profiling

The postbiotic sodium butyrate synergizes the antiproliferative effects of dexamethasone against the AGS gastric adenocarcinoma cells

Exploring the broad-spectrum pharmacological activity of two less studied Australian native fruits: chemical characterisation using LCMS-driven metabolomics

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