Antiproliferative effect of the Ginkgo biloba extract is associated with the enhancement of cytochrome P450 1B1 expression in estrogen receptor-negative breast cancer cells

Zhao, Xiaodan; Ni, Dong; Man, Hong‐Tao; Fu, Zhonglin; Zhang, Meihong; Kou, Shuang; Ma, Shumei

doi:10.3892/br.2013.150

Cited by 19 publications

(12 citation statements)

References 36 publications

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“…However, no studies have provided conclusive mechanisms of action regarding the effects of GA C13:0 in human MCF-7 and mouse 4T-1 breast cancer cells. In our previous study, the fruit of GB extract showed inhibited proliferation in MCF-7 and MDA-MB-231 breast cancer cells and that accompanied by the enhancement of CYP1B1 mRNA expression [22]. In this study, we isolated GA C13:0 from the GBE and investigated the anti-proliferation, pro-apoptosis and anti-migration effects as well as the possible pivotal regulatory role of CYP1B1 in breast cancer cells.…”

Section: Introductionmentioning

confidence: 88%

Anti-Proliferation, Pro-Apoptosis and Anti-Migration Effects of Ginkgolic Acid C13:0 Isolated from Ginkgo Biloba Exocarp in MCF-7 and 4T-1 Breast Cancer Cells

Zhou¹,

Jiang²,

Fu³

et al. 2018

Preprint

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Ginkgolic acids (GA) have been reported to exhibit anticancer properties, however, 15 the mechanisms remain unclear. This study aims to investigate the mechanisms of GA C13:0 16 that was isolated from Ginkgo biloba exocarp (GBE) for anti-proliferation, pro-apoptosis and 17anti-migration effects in human MCF-7 and mouse 4T-1 breast cancer cells. The cytotoxic 18 effect, apoptosis induction and migration inhibition were measured using MTT, TUNEL and 19Wound healing assays. The expression of mRNA and protein were determined using qPCR 20 and Western blot. Our results showed that no cytotoxicity was found at concentrations of 21 C13:0 below 100µM. The effects of GA C13:0 was further demonstrated by up-regulation of 22 the Bax/Bcl-2 apoptosis pathway and the expression of Apaf-1 protein in the mitochondria. In 23 addition, GA C13:0 also suppressed cell migration and epithelial to mesenchymal transition 24 (EMT) with the increase of E-cadherin expression accompanied by the decrease of Snail, 25 MMP-2, MMP-9 and Vimentin expression. Moreover, GA C13:0 induced cytochrome P450 26 (CYP) 1B1 expression in aryl hydrocarbon receptor (AhR) pathway. Notably, the 27 up-regulation of CYP1B1 also might play a pivotal regulatory role in mitochondrial and EMT 28 pathways in MCF-7 and 4T-1 cells. Our results may have implications for the development of 29 anticancer agents containing GA as functional additives. 30

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Section: Introductionmentioning

confidence: 88%

Anti-Proliferation, Pro-Apoptosis and Anti-Migration Effects of Ginkgolic Acid C13:0 Isolated from Ginkgo Biloba Exocarp in MCF-7 and 4T-1 Breast Cancer Cells

Zhou¹,

Jiang²,

Fu³

et al. 2018

Preprint

Self Cite

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“…Shi et al [ 39 ] examined the effects of EGb 761 on platelets and hippocampal mitochondria isolated from SMAP8 mice of different age groups. SMAP8 mice were a senescence-accelerated strain of mice.…”

Section: Pharmacological Actions Of Egbmentioning

confidence: 99%

“…EGb is also involved in several other cancers including breast cancer, non-small cell lung cancer (NSCLC), colon cancer and prostate cancer. Zhao et al [ 39 ] reported that GEb treatment could increase the expression of cytochrome P450(CYP)1B1 in MDA-MB-231 human breast cancer cells. Park et al [ 103 ] also found that EGb mediated cytotoxic effects in the breast cancer cell line MDA-MB-231 by activating caspase-3 and altering the mRNA levels of apoptosis-related genes Bcl-2 and Bax.…”

Section: The Effect Of Egb On Aging-related Diseasesmentioning

confidence: 99%

Advances in the Studies of Ginkgo Biloba Leaves Extract on Aging-Related Diseases

Zuo¹,

Yan²,

Zhang³

et al. 2017

Aging and disease

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The prevalence of degenerative disorders in public health has promoted in-depth investigations of the underlying pathogenesis and the development of new treatment drugs. Ginkgo biloba leaves extract (EGb) is obtained from Ginkgo biloba leaves and has been used for thousands of years. In recent decades, both basic and clinical studies have established the effects of EGb. It is widely used in various degenerative diseases such as cerebrovascular disease, Alzheimer’s disease, macroangiopathy and more. Here, we reviewed several pharmacological mechanisms of EGb, including its antioxidant properties, prevention of mitochondrial dysfunctions, and effect on apoptosis. We also described some clinical applications of EGb, such as its effect on neuro and cardiovascular protection, and anticancer properties. The above biological functions of EGb are mainly focused on aging-related disorders, but its effect on other diseases remains unclear. Thus, through this review, we aim to encourage further studies on EGb and discover more potential applications

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“…EGB is well known for its antioxidant and antiinflammatory activities (Ren et al, 2013). Previous studies showed that EGB may have antitumor effects that are related to its antioxidant, antiangiogenic, and gene-regulatory actions (Dias et al, 2013;Park et al, 2013;Zhao et al, 2013). Flavonoids and terpenoids are major biologically active components of EGB.…”

Section: Introductionmentioning

confidence: 99%

Reference gene expression in human osteosarcoma cell lines treated by EGB and CTX

Ren¹,

Yang²,

Song³

et al. 2017

Turk J Biol

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The quantitative real-time polymerase chain reaction (qRT-PCR) assay is frequently used to study gene expression and is currently considered the gold standard. A stably expressed endogenous gene is often used as an internal control and reference to calculate the relative levels of target genes.It is important to appropriately select reference genes. The ideal reference genes should be stably expressed under nearly all experimental conditions (Radonic et al., 2004;Derveaux et al., 2010). Cellular maintenance genes, such as glyceraldehyde 3-phosphate dehydrogenase (GAPDH), β-actin (ACTB), and ribosomal RNA (18S rRNA), are frequently selected as reference genes. However, several studies have established a variation in the expression levels of these commonly used reference genes in different tissues. Moreover, there could be variations in the expression levels of these genes when they are subjected to different treatments in the same tissue (

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Antiproliferative effect of the Ginkgo biloba extract is associated with the enhancement of cytochrome P450 1B1 expression in estrogen receptor-negative breast cancer cells

Cited by 19 publications

References 36 publications

Anti-Proliferation, Pro-Apoptosis and Anti-Migration Effects of Ginkgolic Acid C13:0 Isolated from Ginkgo Biloba Exocarp in MCF-7 and 4T-1 Breast Cancer Cells

Anti-Proliferation, Pro-Apoptosis and Anti-Migration Effects of Ginkgolic Acid C13:0 Isolated from Ginkgo Biloba Exocarp in MCF-7 and 4T-1 Breast Cancer Cells

Advances in the Studies of Ginkgo Biloba Leaves Extract on Aging-Related Diseases

Reference gene expression in human osteosarcoma cell lines treated by EGB and CTX

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