Antiproliferative effect of alpinetin in BxPC-3 pancreatic cancer cells

Du, Jian; Tang, Bo; Wang, Jingwen; Sui, Hongtao; Jin, Xueli; Wang, Liming; Wang, Zhongyu

doi:10.3892/ijmm.2012.884

Cited by 32 publications

(20 citation statements)

References 31 publications

(30 reference statements)

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“…1A) is a natural flavonoid primarily derived from the roots or seeds of Alpinia katsumadai Hayata that is known to exhibit antibacterial, anti-inflammatory and other important therapeutic properties (12)(13)(14)(15)(16)(17)(18). In previous studies, alpinetin was reported to inhibit proliferation of human tumor cells, including lung cancer and gastroenteric cancer cells (12)(13)(14)(15)(16)19), indicating the potential anticancer properties of this compound. However, the effects of alpinetin on ovarian cancer cells and its underlying mechanisms of action remain largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Alpinetin inhibits proliferation and migration of ovarian cancer cells via suppression of STAT3 signaling

et al. 2018

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Natural bioactive components are increasingly being applied in cancer research. Alpinetin is a type of natural flavonoid primarily derived from Alpinia katsumadai Hayata, which exhibits anti‑bacterial and anti‑inflammatory properties. Therefore, it may possess anticancer potential and be employed therapeutically for different diseases. The aim of the present study was to investigate the anticancer effects of alpinetin on the SKOV3 ovarian cancer cell line. The effect of alpinetin treatment on SKOV3 cell proliferation, apoptosis, spheroid and colony formation were measured using Cell Counting kit‑8, cell apoptosis, 3D spheroid and colony formation assays, respectively. Analysis of the cell cycle was performed using flow cytometry. Western blot analysis was used to determine the protein expression levels of B‑cell lymphoma (Bcl)‑2, Bcl‑2‑associated X protein, cleaved caspase‑3, cleaved poly (ADP‑ribose) polymerase (PARP), cyclin D1, cyclin‑dependent kinase (CDK) 4, CDK6, signal transducer and activator of transcription (STAT) 3, phosphorylated (p)‑STAT3, c‑myc, survivin, tissue inhibitor of metalloproteinase (TIMP)‑1, TIMP‑2, matrix metalloproteinase (MMP)‑2 and MMP‑9. In addition, a wound healing assay was used to determine cancer cell migration. The results revealed alpinetin suppressed cell viability and induced apoptosis of SKOV3 cells in a dose‑ and time‑dependent manner, and cells were arrested in the G1 phase. Alpinetin treatment upregulated protein expression levels of Bax, cleaved caspase‑3 and PARP, and downregulated protein expression levels of Bcl‑2, cyclin D1, CDK4 and CDK6. Alpinetin also inhibited cell migration, through increased protein expression levels of TIMP‑1 and TIMP‑2, and decreased protein expression levels of MMP‑2 and MMP‑9. Alpinetin also significantly suppressed colony and spheroid formation by SKOV3 cells. In addition, the STAT3 pathway was suppressed as demonstrated by downregulation of p‑STAT3 and reduced expression of downstream factors, including c‑myc and survivin. Overall, these results indicated that alpinetin may have anticancer effects on human ovarian cancer by inhibiting the STAT3 signaling pathway.

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Section: Introductionmentioning

confidence: 99%

Alpinetin inhibits proliferation and migration of ovarian cancer cells via suppression of STAT3 signaling

et al. 2018

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“…According to another report, apoptosis of brain glioma cells is closely related to the mitochondrial pathway (16)(17)(18). The inter-shifting of Bcl-2 and Bax proteins releases cytochrome c and activates the caspase family to finally induce apoptosis (19,20).…”

Section: Introductionmentioning

confidence: 99%

“…A Perkin-Elmer LS-50B fluorospectrophotometer was used to measure the alterations in fluorescence intensity when the excitation wavelength was 380 nm and emission wavelength was 460 nm. Previously described methods were used (19).…”

Section: Introductionmentioning

confidence: 99%

Embelin-induced brain glioma cell apoptosis and cell cycle arrest via the mitochondrial pathway

Wang

Zhang

et al. 2013

Oncology Reports

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Abstract. Brain glioma is the most common malignant intracranial tumor and has become the focus of research on diseases of the central nervous system due to its high incidence and poor prognosis. As a small-molecule inhibitor of X-linked inhibitor of apoptosis protein (XIAP), embelin has the ability to specifically inhibit XIAP to control and regulate the apoptosis of various types of tumor cells. However, to date, the mechanism of action for this effect is not well understood. The aim of this study was to investigate the role that the mitochondrial pathway plays in embelin-induced brain glioma cell apoptosis and the effect of embelin on the cell cycle. Brain glioma cells were treated with different doses of embelin. The MTT method

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“…Detection of caspase-3 and -9 activities was performed as described previously (13). The Perkin-Elmer LS-50B fluorescence spectrophotometer (Waltham, MA, USA) was used to measure changes in fluorescence intensity at excitation and emission wavelengths of 380 and 460 nm, respectively.…”

Section: Detection Of Apoptosis By Annexin V-fitc/pi Double Stainingmentioning

confidence: 99%

Alpinetin promotes Bax translocation, induces apoptosis through the mitochondrial pathway and arrests human gastric cancer cells at the G2/M phase

Wang

Lü

et al. 2012

Molecular Medicine Reports

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Abstract. Alpinetin is a natural flavonoid widely distributed inZingiberaceae. Previous studies have demonstrated that alpinetin markedly inhibits tumour growth. However, the molecular mechanisms underlying the antitumour effects of alpinetin are unclear. Bcl-2-associated X protein (Bax) translocation is known to activate the mitochondrial-dependent endogenous apoptosis pathway. The aim of the current study was to investigate the roles of Bax and the mitochondrial pathway during alpinetin-induced gastric cancer cell apoptosis and the effects of alpinetin on the cell cycle. Human gastric cancer cells were treated with various doses of alpinetin and an MTT assay was performed to measure cell viability, flow cytometry to measure the apoptotic rate, changes in the cell cycle and mitochondrial membrane potential and western blot analysis to detect the expression levels of relevant proteins. Results demonstrate that alpinetin induces apoptosis in human gastric cancer cells in a dose-and time-dependent manner. During the early stages of apoptosis, alpinetin may alter mitochondrial membrane potential leading to release of cytochrome c from mitochondria, activation of caspase family members and ultimately apoptosis of human gastric cancer cells. Results of the present study indicate that alpinetin-induced human gastric cancer cell apoptosis is associated with the mitochondrial pathway.

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Antiproliferative effect of alpinetin in BxPC-3 pancreatic cancer cells

Cited by 32 publications

References 31 publications

Alpinetin inhibits proliferation and migration of ovarian cancer cells via suppression of STAT3 signaling

Alpinetin inhibits proliferation and migration of ovarian cancer cells via suppression of STAT3 signaling

Embelin-induced brain glioma cell apoptosis and cell cycle arrest via the mitochondrial pathway

Alpinetin promotes Bax translocation, induces apoptosis through the mitochondrial pathway and arrests human gastric cancer cells at the G2/M phase

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