Antiproliferative, cytotoxic and apoptotic activity of the bentonite transformation of sesquiterpene lactone glaucolide B to 5β-hydroxy-hirsutinolide on tumor cell lines

Flores-Guzmán, Fernando; Alvarado-Sansininea, J. Javier; López-Muñoz, Hugo; Escobar, María Luisa; Espinosa-Trejo, Mayra; Tavera-Hernández, Rosario; Manuel, José; Sánchez-Sánchez, Luis

doi:10.1016/j.ejphar.2019.172406

Cited by 6 publications

(7 citation statements)

References 32 publications

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“…When cultured cells undergo apoptosis, the cell membrane is the rst to be destroyed, and then various enzymes, including LDH, are released and can be rapidly detected in the culture medium (Flores-Guzmán et al 2019). Accordingly, the rate of released LDH can be used to evaluate the integrity of cell membranes (He et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Brevilaterin B from Brevibacillus laterosporus has selective antitumor activity and induces apoptosis in epidermal cancer

Zhou

Wang

Liu

et al. 2022

World J Microbiol Biotechnol

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Brevibacillus laterosporus, a newly discovered genus, had been shown to be one of the best candidates for producing multiple antimicrobial peptides (AMPs). Herein, we discovered a new bioactivity of Brevilaterin B, an AMP produced by Br. laterosporus S62-9, and investigated the details about its Anticancer. Proliferation, membrane permeability and apoptotic rate were checked using CCK-8 Assay, LDH Assay and Annexin V-FITC/PI Kits. ROS levels and mitochondrial membrane potential were detected using the uorescent probe DCFH-DA and JC-1. Brevilaterin B exhibited broad anticancer activity in a dose-dependent manner. It selectively inhibited the proliferation of epidermal cancer cell A431 but had no effect on normal cells at 2 µg/mL. Typical morphological characteristics of apoptosis and an apoptotic ratio of 71.0% in A431 were observed after treatment with 2-3 µg/mL of Brevilaterin B. In A431 cells, 21.3% ROS generated and 48.8% reduction in mitochondrial membrane potential further occurred, indicating the main site of action was the mitochondrion. Brevilaterin B secreted by Br. laterosporus S62-9 has potential application as an anticancer medicament, increasing its commercial value. It's believed to be the rst report of the anticancer activity of this type of AMPs. HighlightsBrevilaterin B was rst report to exhibit anticancer activity. Brevilaterin B induces apoptosis of epidermal cancer cell by action on mitochondrion.Brevilaterin B has potential application as an anticancer medicament.

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Section: Discussionmentioning

confidence: 99%

Brevilaterin B from Brevibacillus laterosporus has selective antitumor activity and induces apoptosis in epidermal cancer

Zhou

Wang

Liu

et al. 2022

World J Microbiol Biotechnol

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“…To determine the anticancer activity of PS14, total LDH release was evaluated. When cellular damage occurs, in response to it, LDH gets released to the growth medium because of the increase in the pore size of the cell membrane [ 24 ]. PS14 treatment showed a dose-dependent increase in the concentration of LDH release than the control.…”

Section: Resultsmentioning

confidence: 99%

Anti-Cancer and Anti-Inflammatory Activities of a Short Molecule, PS14 Derived from the Virulent Cellulose Binding Domain of Aphanomyces invadans, on Human Laryngeal Epithelial Cells and an In Vivo Zebrafish Embryo Model

et al. 2022

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In this study, the anti-cancer and anti-inflammatory activities of PS14, a short peptide derived from the cellulase binding domain of pathogenic fungus, Aphanomyces invadans, have been evaluated, in vitro and in vivo. Bioinformatics analysis of PS14 revealed the physicochemical properties and the web-based predictions, which indicate that PS14 is non-toxic, and it has the potential to elicit anti-cancer and anti-inflammatory activities. These in silico results were experimentally validated through in vitro (L6 or Hep-2 cells) and in vivo (zebrafish embryo or larvae) models. Experimental results showed that PS14 is non-toxic in L6 cells and the zebrafish embryo, and it elicits an antitumor effect Hep-2 cells and zebrafish embryos. Anticancer activity assays, in terms of MTT, trypan blue and LDH assays, showed a dose-dependent inhibitory effect on cell proliferation. Moreover, in the epithelial cancer cells and zebrafish embryos, the peptide challenge (i) caused significant changes in the cytomorphology and induced apoptosis; (ii) triggered ROS generation; and (iii) showed a significant up-regulation of anti-cancer genes including BAX, Caspase 3, Caspase 9 and down-regulation of Bcl-2, in vitro. The anti-inflammatory activity of PS14 was observed in the cell-free in vitro assays for the inhibition of proteinase and lipoxygenase, and heat-induced hemolysis and hypotonicity-induced hemolysis. Together, this study has identified that PS14 has anti-cancer and anti-inflammatory activities, while being non-toxic, in vitro and in vivo. Future experiments can focus on the clinical or pharmacodynamics aspects of PS14.

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“…Among species of Vernonia, antitumor properties have been described Vernonia amygdalina [5,11,47,48,49], Vernonia bockiana [50], Vernonia cinerea [51,52,53], Vernonia condensate [54], Vernonia divaricata [43], Vernonia extensa [44], Vernonia fimbrillifera [55], Vernonia glaberrima [56,57], Vernonia kotschyana [58], Vernonia leopoldii [59], Vernonia mespilifolia [60], Vernonia pachyclada [61], Vernonia paniculata [62], Vernonia patula [63], Vernonia polyanthes [64], and Vernonia scorpioides [65,66,67], Vernonia zeylanica [42,68].…”

Section: Genus Antitumor Activitymentioning

confidence: 99%

Genus Vernonia (Asteraceae): A Promising Source of Antitumor Agents with Pharmacological Potentials

Gonzatto

Araújo²,

Albuquerque³

2022

AIR

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Antitumor research leads to the development of new molecules that act specifically in tumor cells by blocking or inhibiting their molecular targets. New therapeutic approaches for the screening of bioactive compounds present in medicinal plants have received increasing attention due to their chemopreventive and chemotherapeutic properties. In ethnomedicine, plants of the genus Vernonia (Asteraceae) are widely used and some have shown several and interesting biological activities, including anticancer. This present study aimed to document experimental evidence supporting the claimed ethnomedical uses of Vernonia species for the treatment of various types of cancer and also to confirm the anticancer potential of these plants. The compounds isolated from aqueous and alcoholic extracts, as well as fractions from different parts of Vernonia plants have acted as potential anticancer agents that inhibited the proliferation of various types of human cancer cell lines, including cervical cancer cells, melanoma cells, promyelocytic leucemia cells, breast adenocarcinoma cells, ovarian cancer, liver cancer cell, and human lung cancer cells. Studies have correlated the antitumor activity of Vernonia plants by inducing apoptosis and modulating mitochondrial signaling pathways controlled by NF-κB, Bcl-2 and p53, as well as inducing DNA damage and arresting the cell cycle at the S-phase checkpoint by oxidative stress. In conclusion, Vernonia species act as a promising source for drug development. However, further studies are needed to explore the exact mechanism of action, pharmacokinetics, chronic toxicological studies, safe dose consumption, and possible interactions with other herbs.

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Antiproliferative, cytotoxic and apoptotic activity of the bentonite transformation of sesquiterpene lactone glaucolide B to 5β-hydroxy-hirsutinolide on tumor cell lines

Cited by 6 publications

References 32 publications

Brevilaterin B from Brevibacillus laterosporus has selective antitumor activity and induces apoptosis in epidermal cancer

Brevilaterin B from Brevibacillus laterosporus has selective antitumor activity and induces apoptosis in epidermal cancer

Anti-Cancer and Anti-Inflammatory Activities of a Short Molecule, PS14 Derived from the Virulent Cellulose Binding Domain of Aphanomyces invadans, on Human Laryngeal Epithelial Cells and an In Vivo Zebrafish Embryo Model

Genus Vernonia (Asteraceae): A Promising Source of Antitumor Agents with Pharmacological Potentials

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