Antiproliferative, Antioxidant, Chemopreventive and Antiangiogenic Potential of Chromatographic Fractions from Anemonia sulcata with and without Its Symbiont Symbiodinium in Colorectal Cancer Therapy

Abstract

Anemonia sulcata may be a source of marine natural products (MNPs) due to the antioxidant and antitumor activity of its crude homogenates shown in vitro in colon cancer cells. A bioguided chromatographic fractionation assay of crude Anemonia sulcata homogenates with and without its symbiont Symbiodinium was performed to characterize their bioactive composition and further determine their biological potential for the management of colorectal cancer (CRC). The 20% fractions retained the in vitro antioxidant activity previously reported for homogenates. As such, activation of antioxidant and detoxifying enzymes was also evaluated. The 40% fractions showed the greatest antiproliferative activity in T84 cells, synergistic effects with 5-fluoruracil and oxaliplatin, overexpression of apoptosis-related proteins, cytotoxicity on tumorspheres, and antiangiogenic activity. The predominantly polar lipids and toxins tentatively identified in the 20% and 40% fractions could be related to their biological activity in colon cancer cells although further characterizations of the active fractions are necessary to isolate and purify the bioactive compounds.

Experimental Tumor Induction and Evaluation of Its Treatment in the Chicken Embryo Chorioallantoic Membrane Model: A Systematic Review

“…In biomedicine, as discussed in this systematic review, its application in tumor induction studies and testing of antitumor treatments is widespread [37]. In addition, many studies focus on the search for antiangiogenic drugs using the CAM vascular network [89]. Recently, the CAM has started to be used in tissue engineering for organoid implantation.…”

Proteomic Approach to Anemonia sulcata and Its Symbiont Symbiodinium spp. as New Source of Potential Biotechnological Applications and Climate Change Biomarkers

“…Sequence characterized amplified region (SCAR) data indicate that the vast majority of Anemonia worldwide (encompassing two described species, A. sucata (Pennant, 1777) and A. viridis (Forsskål, 1775), appear to be genetically homogeneous. Another relevant reason to select these organisms is based on the recent discovery of molecules with antiproliferative, antioxidant, chemopreventive and antiangiogenic potential against colorectal cancer [ 12 ], including some peptides, which could be a clue to the presence of proteins with similar activities related to biomedicine or agri-food. Additionally, as has been commented, when a bioindicator species is proposed, it should demonstrate its importance as a blue economy supporting input.…”