Antiproliferative and Apoptosis Induction Potential of the Methanolic Leaf Extract of<i>Holarrhena floribunda</i>(G. Don)

Badmus, Jelili A.; Ekpo, Okobi Eko; Hussein, Ahmed A.; Meyer, Mervin; Hiss, Donavon C

doi:10.1155/2015/756482

Cited by 43 publications

(26 citation statements)

References 31 publications

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“…From the results obtained in Figures 5 and 6 there was no direct relationship in the induction levels of caspase 9 and caspase 3. e activation of caspase 9 was four times lower than that observed in caspase 3 in RD cells. Badmus and others reported a low caspase 9 induction effect of Holarrhena floribunda (G. Don) extracts on the breast (MCF-7), colorectal (HT-29), and cervical (HeLa) cancer cells [32]. Our study also observed a low activity of caspase 9 in 8…”

Section: Evidence-based Complementary and Alternative Medicinesupporting

confidence: 69%

Chemopreventive Effects and Antioxidant Capacity of Combined Leaf Extracts ofSesamum angustifolium(Oliv.) Engl. andHibiscus articulatuson Rhabdomyosarcoma

Siamayuwa

Nyanga

Chidewe

2020

Evidence-Based Complementary and Alternative Medicine

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Sesamum angustifolium (Oliv.) Engl. and Hibiscus articulatus contain compounds that have antimutagenic properties. The rise in rhabdomyosarcoma in paediatrics and prognosis of the disease in infants compared to adults calls for newer, less toxic alternatives in treatment of the disease. The aim of this study was to determine the anticancer activity and antioxidant capacity of combined leaf extracts of Sesamum angustifolium (Oliv.) Engl. and Hibiscus articulatus (SAHA), against rhabdomyosarcoma (RMS) using rhabdomyosarcoma (RD) cell line and mouse (L20B) cell line. Cytotoxicity, morphology, apoptosis induction, and antioxidant capacity assays were done. Of the four solvents used for extraction, the dichloromethane SAHA extract was the most cytotoxic with IC50 of 106 μg/mL after doxorubicin, the reference anticancer drug with IC50 of 0.8 μg/mL. The SAHA extracts had a stronger cytotoxicity effect on the cancerous RD cells than on normal L20B cells. Morphological assessment showed untreated cells maintained their normal striated appearance of muscle cells whereas cells treated with doxorubicin or SAHA extracts exhibited cell shrinkage, loss of surface adherence, reduced cell density along with cell debris, which is a characteristic of apoptosis. Normal L20B cells when treated with doxorubicin or SAHA extracts, maintained their cell shape, and remained adherent to the surface. The apoptotic enzyme caspase-3 was induced in a concentration dependent manner upon treatment of the RD cells with SAHA extracts or doxorubicin. Induction of caspase-3 was ten times less in treated L20B cells compared to the RD cells. Low induction of caspase-9 enzyme was observed in both treated RD and L20B cells. Treatment of both RD and L20B cells with SAHA extracts or doxorubicin resulted in increased activity of peroxidase and reduction of oxidative stress. Results of the study show that the SAHA extracts are potential sources of compounds that may serve as useful agents for treatment of rhabdomyosarcoma.

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Section: Evidence-based Complementary and Alternative Medicinesupporting

confidence: 69%

Chemopreventive Effects and Antioxidant Capacity of Combined Leaf Extracts ofSesamum angustifolium(Oliv.) Engl. andHibiscus articulatuson Rhabdomyosarcoma

Siamayuwa

Nyanga

Chidewe

2020

Evidence-Based Complementary and Alternative Medicine

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“…According to the statistics by the Malaysian Ministry of Health, the annual mortality rate for cancer disease has reached more than 10% since 2006 [ 4 ]. More than 60% of currently used drugs for cancer treatment are natural based products [ 5 ]. The United States National Cancer Institute (NCI) has collected about 35,000 plants and screened 114,000 natural products extracts for anticancer studies [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Constituents, Antioxidant and Antiproliferative Properties of a Liverwort,Lepidozia borneensisStephani from Mount Kinabalu, Sabah, Malaysia

Bakar

Suleiman

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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The study aimed to investigate the phytochemical contents, antioxidant and antiproliferative activity of 80% methanol extract of Lepidozia borneensis. The total phenolic and total flavonoid contents were analysed using Folin-Ciocalteu and aluminium chloride colorimetric methods. Antioxidant properties were evaluated by using FRAP, ABTS, and DPPH assays while the effects of L. borneensis on the proliferation of MCF-7 cell line were evaluated by using MTT assay. The results showed that the total phenolic and flavonoid contents were 12.42 ± 0.47 mg GAE/g and 9.36 ± 1.29 mg CE/g, respectively. The GC-MS analysis revealed the presence of at least 35 compounds. The extract was found to induce cytotoxicity against MCF-7 cell line with IC50 value of 47.33 ± 7.37 µg/mL. Cell cycle analysis showed that the extract induced significant arrest at G0/G1 at 24 hours of treatment. After 72 hours of treatment, the proportion of cells in G0/G1 and G2-M phases had decreased significantly as compared to their control. Apoptosis occurred during the first 24 hours and significantly increased to 30.8% after 72 hours of treatment. No activation of caspase 3 was observed. These findings suggest that L. borneensis extract has the potential as natural antioxidant and anticancer agents.

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“…In the present study, PBEA exhibited the highest cytotoxic activity with IC 50 value of 14.37 ± 8.40 lg/ml and selectively against HeLa cells while no cytotoxicity was observed in normal cells. The ability of the extract to distinguish between normal and malignant cells is a crucial aspect in the development of an anticancer agent (29). The characteristic of PBEA is consistent with this concept hence possess high potential to become an anticancer agent for cervical cancer.…”

Section: Discussionmentioning

confidence: 55%

Pereskia bleo Leaves Extract Induces Cell Death via Cell Cycle Arrest and Apoptosis in Cervical Cancer Cells HeLa

Mohd-Salleh

Wan-Ibrahim

Ismail

2019

Nutrition and Cancer

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Introduction: Pereskia bleo is a leafy and edible plant, locally known as "Pokok Jarum Tujuh Bilah" which has anticancer properties. This study purposed to determine the cytotoxic effects of P. bleo leaves extracts on several well-known cancer cells and elucidate its underlying mechanism in inducing cell death. Methods: Cytotoxic activity on selected cell lines was determined using MTT assay. Mechanism of cell death was investigated through cell cycle and Annexin V assay. Expression of apoptotic proteins was measured by flow cytometry method. Results: Ethyl acetate extract of P. bleo leaves (PBEA) appeared to have the strongest IC 50 value (14.37 ± 8.40 lg/ml) and most active against HeLa cells was further studied for apoptosis. The cell cycle investigation by flow cytometry evidenced the increment of PBEA treated HeLa cells in G 0 /G 1 phase and apoptotic event was detected in Annexin V assay. Analysis of apoptotic protein showed pro-apoptotic proteins (Bax, p53 and caspase 3) were triggered where as anti-apoptotic protein Bcl-2 was suppressed in treated HeLa cells. Conclusions: Our findings demonstrated that PBEA treatment induced cell death in HeLa cells by p53-mediated mechanism through arresting cell cycle at G 0 /G 1 phase and mitochondrial-mediated pathway with involvement of pro-apoptotic proteins, anti-apoptotic protein, and caspase 3.

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Antiproliferative and Apoptosis Induction Potential of the Methanolic Leaf Extract ofHolarrhena floribunda(G. Don)

Cited by 43 publications

References 31 publications

Chemopreventive Effects and Antioxidant Capacity of Combined Leaf Extracts ofSesamum angustifolium(Oliv.) Engl. andHibiscus articulatuson Rhabdomyosarcoma

Chemopreventive Effects and Antioxidant Capacity of Combined Leaf Extracts ofSesamum angustifolium(Oliv.) Engl. andHibiscus articulatuson Rhabdomyosarcoma

Phytochemical Constituents, Antioxidant and Antiproliferative Properties of a Liverwort,Lepidozia borneensisStephani from Mount Kinabalu, Sabah, Malaysia

Pereskia bleo Leaves Extract Induces Cell Death via Cell Cycle Arrest and Apoptosis in Cervical Cancer Cells HeLa

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