Antiproliferative and antioxidant potentials of bioactive edible vegetable fraction of Achyranthes ferruginea Roxb. in cancer cell line

Abstract

In the present study, the aerial parts of Achyranthes ferruginea underwent investigation of their in vitro antioxidant and free radical‐scavenging activities in cell‐free conditions, their phytoconstituents using gas chromatography‐mass spectrometry (GC‐MS), and their cytotoxic activity in HeLa cells. A. ferruginea was extracted with 80% methanol and successively fractionated with solvents to yield petroleum ether (PEF), chloroform (CHF), ethyl acetate (EAF), and aqueous (AQF) fractions. GC‐MS analysis revealed that CHF contained ten phytoconstituents, including different forms of octadecanoic acid methyl esters. The total antioxidant and ferric‐reducing antioxidant capacities of the extracts and the standard catechin (CA) were as follows: CA >CHF >PEF >CME (crude methanolic extract) >EAF >AQF, and CA >CHF >EAF >PEF >AQF >CME, respectively. CHF showed the highest DPPH‐free radical‐scavenging activity, with a median inhibitory concentration of 10.5 ± 0.28 µg/ml, which was slightly higher than that of the standard butylated hydroxytoluene (12.0 ± 0.09 µg/ml). In the hydroxyl radical‐scavenging assay, CHF showed identical scavenging activity (9.25 ± 0.73 µg/ml) when compared to CA (10.50 ± 1.06 µg/ml). Moreover, CHF showed strong cytotoxic activity (19.95 ± 1.18 µg/ml) in HeLa cells, which was alike to that of the standards vincristine sulfate and 5‐fluorouracil (15.84 ± 1.64 µg/ml and 12.59 ± 1.75 µg/ml, respectively). The in silico study revealed that identified compounds were significantly linked to the targets of various cancer cells and oxidative enzymes. However, online prediction by SwissADME, admetSAR, and PASS showed that it has drug‐like, nontoxic, and potential pharmacological actions.

Traditional uses, pharmacological activities, and phytochemical constituents of the genus Syzygium: A review

“…The genus Syzygium is one of the genera of the myrtle family Myrtaceae comprising 1200–1800 species spread out over the world (B. Ahmad et al., 2016 ; Reza et al., 2021 ). The genus Syzygium (Myrtaceae) is named after a Greek word meaning “coupled,” an illusion to the paired branches and leaves (Nigam & Nigam, 2012 ).…”

Pharmacological insights into Chukrasia velutina bark: Experimental and computer‐aided approaches

“…Oxidation is responsible for many diseases related to pain such as cancer, infection, arthritis, cardiovascular diseases and type‐2 diabetes. 16 Antioxidant containing chemical compounds have been shown to reduce oxidation in cells of the body. 26 In the present study, we evaluated the antioxidant effect of MECVB.…”

“…TPC was assessed spectrophotometrically by measuring absorbance at 760 nm. 16 A 1 ml methanol solution of extract was mixed with 2.5 ml FCR and 2.5 ml Na 2 CO 3 and incubated at 25°C for about 20 min, after which the absorbance of the solution was measured. Three measurements were taken to determine the mean absorbance, providing the TPC value of extract solution.…”

GC/MS Analysis, Antioxidant Activity, and Antimicrobial Effect of Pelargonium peltatum (Geraniaceae)

“…The fractionation of the FDE-Leaf and Flower was performed by Emran et al’s method [ 69 ] with minor modifications, 5 g of each extract were resuspended in 200 mL of ethanol 70%, then the solution was divided into four parts with 200 mL of different organic solvents varying its polarity (hexane, chloroform, ethyl acetate, and methanol). These new solutions were put under agitation for 10 min, and poured into a decantation funnel for the separation of their phases.…”