Antiproliferative and Antimetastatic Effects of Praeruptorin C on Human Non–Small Cell Lung Cancer through Inactivating ERK/CTSD Signalling Pathways

Liu, Chien Ming; Shen, Huan; Lin, Yi; Yu, Yung Luen; Chen, Yong Syuan; Liu, Chung‐Jung; Hsieh, Yi‐Hsien

doi:10.3390/molecules25071625

Cited by 15 publications

(8 citation statements)

References 46 publications

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“…Some other studies had demonstrated that praeruptorin A could inhibit the migration and invasion of liver cancer cells, and inhibit the expression of the MMP1 gene by activating the extracellular signal-regulated kinase (ERK) signaling pathway, thereby inhibiting the movement of liver cancer cells ( Yu et al, 2020 ). Praeruptorin C had good pharmacological effects in anti-inflammatory, antihypertensive, and antiplatelet aggregation ( Liu et al, 2020 ). Studies had shown that praeruptorin C could significantly inhibit the proliferation, colony formation, wound healing, and migration of the non-small cell lung cancer cells.…”

Section: Introductionmentioning

confidence: 99%

“…Studies had shown that praeruptorin C could significantly inhibit the proliferation, colony formation, wound healing, and migration of the non-small cell lung cancer cells. By inhibiting the phosphorylation of the ERK1/2 signaling pathway, it apparently reduced the expression of cathepsin D and thereby inhibited the invasion activity of the non-small cell lung cancer cells ( Liu et al, 2020 ). In addition, praeruptorin C has a good therapeutic role in improving neuroprotection such as motor and cognitive impairment in Huntington’s disease ( Wang et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

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De novo Transcriptome Sequencing Coupled With Co-expression Analysis Reveal the Transcriptional Regulation of Key Genes Involved in the Formation of Active Ingredients in Peucedanum praeruptorum Dunn Under Bolting Period

Song

Jia

et al. 2021

Front. Genet.

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Peucedanum praeruptorum Dunn is a perennial and one-off flowering plant of the Peucedanum genus in Umbelliferae. The cultivated P. praeruptorum Dunn usually grows nutritionally in the first year and then moves into the reproductive growth in the second year. The lignification of the roots caused by bolting leads to the quality decline of crude materials. Since most of the previous studies have dealt with coumarin biosynthesis and identification of functional genes in P. praeruptorum, the scientific connotation of the inability that the bolted P. praeruptorum cannot be used medically is still unclear. Here, we employed a transcriptome sequencing combined with coexpression analysis to unearth the regulation mechanism of key genes related to coumarin synthesis in pre- and postbolting period, and to explore the mechanisms underlying the effects of bolting on the formation and transport of coumarins between the annual and biennial plants. Six cDNA libraries were constructed, and the transcripts were sequenced and assembled by Illumina Hiseq platform. A total of 336,505 unigenes were obtained from 824,129 non-redundant spliced transcripts. Unigenes (114,488) were annotated to the NCBI nr database, 119,017 and 10,475 unigenes were aligned to Gene Ontology (GO) functional groups and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, respectively. Differential expression analysis screened out a series of upregulated and downregulated genes related to the phenylpropanoid pathway. The heatmap clustering showed that the similar expression patterns were both observed in groups C vs. D and groups C vs. F. The WGCNA-based coexpression was performed to elucidate the module and trait relationship to unearth important genes related to the bolting process. Seven pivotal modules on the KEGG functional annotations suggested these genes were mainly enriched in the process of plant–pathogen interaction, plant hormone signal transduction, MAPK signaling pathway, α-linolenic acid metabolism, circadian rhythm, and phenylpropanoid pathway. Further analysis provided clues that the key genes of the phenylpropanoid pathway, the ABC transporters, the apoptosis-related and circadian rhythm regulatory genes may play pivotal roles in regulating bolting signaling, biosynthesis, and transportation of coumarins.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

De novo Transcriptome Sequencing Coupled With Co-expression Analysis Reveal the Transcriptional Regulation of Key Genes Involved in the Formation of Active Ingredients in Peucedanum praeruptorum Dunn Under Bolting Period

Song

Jia

et al. 2021

Front. Genet.

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“…Following, a novel approaches for the treatment of lung cancer should be discovered for the prevention of lung cancer especially NSCLC associated metastasis. In addition to intricate molecular progression of NSCLC, drug resistance is also are the main roots of mortality in NSCLC patients 24 . Though research found that investigation using human lung cancer model, is the best approach to recognize the vital pathways involved in human NSCLC, apart from the in vitro model and the potential therapeutic drug, such mechanistic study is yet to be limited due to ethical reason.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to intricate molecular progression of NSCLC, drug resistance is also are the main roots of mortality in NSCLC patients. 24 Though research found that investigation using human lung cancer model, is the best approach to recognize the vital pathways involved in human NSCLC, apart from the in vitro model and the potential therapeutic drug, such mechanistic study is yet to be limited due to ethical reason. However, animal models offer an alternative for the exploration of the disease development.…”

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confidence: 99%

Potential chemotherapeutic effect of betalain against human non‐small cell lung cancer through PI3K/Akt/mTOR signaling pathway

Yin

Yang

Guo

et al. 2021

Environmental Toxicology

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This work focuses on evaluating the therapeutic ability of betalain and its causal mechanisms in NSCLC both in vivo and in vitro. The experimental results demonstrated that betalain was able to reduce the viability of A549 cells dose dependently with undetectable toxicity toward normal human cells. Betalain also augmented the apoptotic cells of A549 and cell cycle arrest which was evidenced via increased in level of p53/p21 and decreasing levels of cyclin-D1 complex. Moreover, betalain also reduced the levels of p-PI3K, p-Akt, and mammalian target of rapamycin significantly, justifying the pro-apoptotic effect on A549 cells. The in vivo anticancer activity of betalain was determined further in nude mice injected with A549 cells. Xenograft in vivo experiments confirmed betalain administration of ameliorates the expression of pro-inflammatory cytokines, tumor markers with reduced toxic effect. Accordingly, this combined study provides significant insight on betalain as a therapeutic agent.

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“…Epigallocatechin gallate could inhibit the migration of human uveal melanoma cells via the downregulation of ERK1/2 phosphorylation [37]. Kaempferol inhibits cell migration by targeting ERK1/2 signalling in human retinal pigment epithelial cells [38]. In addition, ERK1/2 mediates the antimetastatic activity of β-mangostin against human hepatocellular carcinoma cells [39].…”

Section: Discussionmentioning

confidence: 99%

Modulating the ERK1/2–MMP1 Axis through Corosolic Acid Inhibits Metastasis of Human Oral Squamous Cell Carcinoma Cells

Chen

Lai

Ying

et al. 2021

IJMS

Self Cite

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Corosolic acid (CA; 2α-hydroxyursolic acid) is a natural pentacyclic triterpenoid with antioxidant, antitumour and antimetastatic activities against various tumour cells during tumourigenesis. However, CA’s antitumour effect and functional roles on human oral squamous cell carcinoma (OSCC) cells are utterly unknown. In this study, our results demonstrated that CA significantly exerted an inhibitory effect on matrix metalloproteinase (MMP)1 expression, cell migration and invasion without influencing cell growth or the cell cycle of human OSCC cells. The critical role of MMP1 was confirmed using the GEPIA database and showed that patients have a high expression of MMP1 and have a shorter overall survival rate, confirmed on the Kaplan–Meier curve assay. In the synergistic inhibitory analysis, CA and siMMP1 co-treatment showed a synergically inhibitory influence on MMP1 expression and invasion of human OSCC cells. The ERK1/2 pathway plays an essential role in mediating tumour progression. We found that CA significantly inhibits the phosphorylation of ERK1/2 dose-dependently. The ERK1/2 pathway played an essential role in the CA-mediated downregulation of MMP1 expression and in invasive motility in human OSCC cells. These findings first demonstrated the inhibitory effects of CA on OSCC cells’ progression through inhibition of the ERK1/2–MMP1 axis. Therefore, CA might represent a novel strategy for treating OSCC.

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Antiproliferative and Antimetastatic Effects of Praeruptorin C on Human Non–Small Cell Lung Cancer through Inactivating ERK/CTSD Signalling Pathways

Cited by 15 publications

References 46 publications

De novo Transcriptome Sequencing Coupled With Co-expression Analysis Reveal the Transcriptional Regulation of Key Genes Involved in the Formation of Active Ingredients in Peucedanum praeruptorum Dunn Under Bolting Period

De novo Transcriptome Sequencing Coupled With Co-expression Analysis Reveal the Transcriptional Regulation of Key Genes Involved in the Formation of Active Ingredients in Peucedanum praeruptorum Dunn Under Bolting Period

Potential chemotherapeutic effect of betalain against human non‐small cell lung cancer through PI3K/Akt/mTOR signaling pathway

Modulating the ERK1/2–MMP1 Axis through Corosolic Acid Inhibits Metastasis of Human Oral Squamous Cell Carcinoma Cells

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