Antiproliferative and anticytotoxic effects of cell fractions and exopolysaccharides from Lactobacillus casei 01

Liu, Chu-Ting; Chu, Fang-Jung; Chou, Chien-Ming; Yu, Roch-Chui

doi:10.1016/j.mrgentox.2011.01.005

Cited by 96 publications

(54 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast to the studies that reported antioxidant effects for some strains of L. casei, and the study that found potential antioxidant properties for L. casei 01 [19,20,[22][23][24]48], the subspecies we applied in our investigation (L. casei 01) seems not to possess the very characteristic, either at all or not to an extent that would be revealed at the low dosage (10 8 CFU/day) and short treatment duration (8 weeks) of our study. The probiotic dosage of our study supplements was lower than that of most of the previous clinical trials.…”

Section: Discussionmentioning

confidence: 88%

“…On the other hand, oxidative stress has been suggested to be implicated in the disease pathogenesis [4] and is reported to play a role in gut microbiota changes as well [38]. Thus, we hypothesized that supplementing the patients with a probiotic strain with probable antioxidant effects [20] may both positively affect the gut flora and reduce oxidative stress in the subjects.…”

Section: Discussionmentioning

confidence: 99%

“…L. casei SY13 had good radical scavenging capacity and inhibited lipid peroxidation in an in vitro study [19]. In another in vitro study investigating cytotoxity of L. casei 01 and its fractions, all the fractions and exopolysaccharides from this strain decreased the cytotoxicity of 4-nitroquinoline 1-oxide (4-NQO) against intestine 407 cells; 4-NQO is a carcinogenic substance that may exert its effect through production of reactive oxygen species [20,21]. Rats fed lyophilized L. casei or milk fermented with L. casei had lower levels of thiobarbituric acid reactive substances (TBARS) in their low-density lipoprotein (LDL) [22].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of Probiotic Supplementation on Oxidative Stress Indices in Women with Rheumatoid Arthritis: A Randomized Double-Blind Clinical Trial

Vaghef-Mehrabany

Rad

Alipour

et al. 2015

Journal of the American College of Nutrition

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Probiotic Supplementation on Oxidative Stress Indices in Women with Rheumatoid Arthritis: A Randomized Double-Blind Clinical Trial

Vaghef-Mehrabany

Rad

Alipour

et al. 2015

Journal of the American College of Nutrition

View full text Add to dashboard Cite

show abstract

“…Previous studies have investigated the potential of EPSs as antiproliferative or anticytoxic agents with intestinal epithelial cells (Ruas-Madiedo et al, 2010;Liu et al, 2011), but, the immunomodulatory effects of EPSs on these cells has largely been neglected in the literature. However, Lebeer et al (2012), as part of a much larger investigation, have reported that EPSs isolated from the known probiotic organism Lactobacillus rhamnosus GG had no significant effect on IL-8 mRNA expression in Caco-2 cells.…”

Section: Exopolysaccharidesmentioning

confidence: 99%

Exploring the immunomodulatory potential of microbial-associated molecular patterns derived from the enteric bacterial microbiota

Patten

Collett

2013

Microbiology

View full text Add to dashboard Cite

The human intestinal lumen represents one of the most densely populated microbial niches in the biological world and, as a result, the intestinal innate immune system exists in a constant state of stimulation. A key component in the innate defence system is the intestinal epithelial layer, which acts not only as a physical barrier, but also as an immune sensor. The expression of pattern recognition receptors, such as Toll-like receptors, in epithelial cells allows innate recognition of a wide range of highly conserved bacterial moieties, termed microbial-associated molecular patterns (MAMPs), from both pathogenic and non-pathogenic bacteria. To date, studies of epithelial immunity have largely concentrated on inflammatory pathogenic antigens; however, this review discusses the major types of MAMPs likely to be produced by the enteric bacterial microbiota and, using data from in vitro studies, animal model systems and clinical observations, speculates on their immunomodulatory potential. IntroductionThe intestine represents the body's largest mucosal surface, with the adult human intestine estimated to cover an area of about 250 m 2 (Artis, 2008). The highly folded luminal surface of the intestinal wall significantly increases absorption efficiency and, as such, is the largest surface area of the body exposed to the environment and its high microbial load (DeSesso & Jacobson, 2001). Humans have co-evolved with indigenous microbial populations, termed microbiota, which inhabit various niches of the body (Hooper et al., 2012) and the adult intestine is one of the most densely populated microbial habitations in the biological world (Artis, 2008). The enteric microbiota predominantly consists of bacteria, with estimated populations of~10 14 bacteria, with up to 500 species represented (Gill et al., 2006;Guarner & Malagelada, 2003); however, methanogenic archaea, eukaryotes (yeasts) and viruses (mainly bacteriophages) are also present (Lozupone et al., 2012). Due to the vast expanse of intestinal tissue exposed to the microbiota, the local innate immune system is in a constant state of stimulation, and a chronic, low-level proinflammatory response is characteristic of enteric immune homeostasis (Macpherson & Harris, 2004;Artis, 2008).The intestinal epithelium plays an active role in innate immunity, and pattern recognition receptors (PRRs) are utilized to detect the presence of bacteria and their associated antigens. PRRs are germline-encoded, sensory molecules which recognize a range of highly conserved bacterial motifs, termed 'pathogen-associated molecular patterns' (PAMPs) (Medzhitov, 2001). However, the ability of PRRs to recognize these bacterial moieties is not limited to just pathogens, and so the term 'microbial-associated molecular patterns' (MAMPs) may be more accurate (Medzhitov, 2001; Sanderson & Walker, 2007) and will be used throughout this review. Epithelium-associated, enteric immune cells, such as macrophages, dendritic cells, T-cells and B-cells, differentially express two major groups of PRRs, th...

show abstract

“…They play an important role in protection against desiccation [2] and also useful in forming biofilms [3,4]. Their various applications include use as gelling agents, biosurfactants, emulsifiers, viscosifiers [5][6][7], biosorbants [8,9], biologically active antimicrobials, anticancer agents and antioxidants [10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Production and Characterization of Exopolysaccharide From Marine Moderately Halophilic Bacterium Halomonas Smyrnensis SVD Iii

Deshmukh

Kanekar²,

Bhadekar

2017

Int J Pharm Pharm Sci

View full text Add to dashboard Cite

Objective: To study 1) Optimization of nutritional and environmental parameters to enhance the yield of EPS by Halomonas smyrnensis SVD III isolated from seawater, West Coast of Maharashtra, India and 2) Purification and characterization of the EPS produced. Methods:The isolate was grown in Sehgal and Gibbons (SG) medium broth supplemented with 3% glucose, at 37 °C, 120 rpm for 7 d. Optimization of different parameters was carried out with one factor at a time approach. EPS was isolated from cell-free supernatant of the culture broth by centrifugation and precipitation using chilled ethanol, after removal of proteins by trichloroacetic acid (TCA) treatment. Characterization of the purified EPS was carried out with respect to fourier-transform infrared (FTIR) spectrum, 1 H nuclear magnetic resonance (NMR) spectrum and mass spectrometry (MS) analysis.Results: Two-fold increase in the yield of EPS (23 g/l) by the selected isolate was obtained by using culture conditions as 10% inoculum size having cell density of 10 7 cells/ml, pH 6, incubation temperature 45 °C, 3% carbohydrate, 0.5% yeast extract as nitrogen source, 20% salt concentration and 7 d of incubation period. Characterization of the purified EPS suggested the presence of dominated glycosidic linkages and heptasaccharide nature of the molecule. As the present strain is halophilic, 20% NaCl was found to be optimum. Conclusion:Optimization studies resulted in two-fold increase in the yield of EPS which is of heptasaccharide nature.

show abstract

Antiproliferative and anticytotoxic effects of cell fractions and exopolysaccharides from Lactobacillus casei 01

Cited by 96 publications

References 23 publications

Effects of Probiotic Supplementation on Oxidative Stress Indices in Women with Rheumatoid Arthritis: A Randomized Double-Blind Clinical Trial

Effects of Probiotic Supplementation on Oxidative Stress Indices in Women with Rheumatoid Arthritis: A Randomized Double-Blind Clinical Trial

Exploring the immunomodulatory potential of microbial-associated molecular patterns derived from the enteric bacterial microbiota

Production and Characterization of Exopolysaccharide From Marine Moderately Halophilic Bacterium Halomonas Smyrnensis SVD Iii

Contact Info

Product

Resources

About