Antiproliferative and Antibacterial Activities of Cirsium scabrum from Tunisia

Abstract

Several Cirsium species are known for their uses in traditional medicine and consequently are studied for their phytochemical content and their biological activities. In the framework of a previous study conducted on eight extremophile plants from Tunisia, we highlighted that the crude methanolic extract of C. scabrum, a not investigated thistle, showed moderate but quite selective cytotoxic activity against the cancerous cell line J774 compared to the noncancerous cell line WI38 (IC50 = 11.53 μg/ml on J774, IC50 = 29.89 µg/ml on WI38, and selectivity index = 2.6). In the current study, the partitions of the leaves of C. scabrum were analyzed for their antiproliferative activity on the same cell lines. From the most active petroleum ether partition, we isolated four triterpenoids including lupeol, taraxasterol acetate, and a (1 : 1) mixture of 25-hydroperoxycycloart-23-en-3β-ol and 24-hydroperoxycycloart-25-en-3β-ol. These two cycloartane-type triterpenoids are mostly responsible for this cytotoxic activity. On the other hand, the antimicrobial potential of this plant was also evaluated against 36 microorganisms. The moderate antibacterial activity against 6 Staphylococcus aureus and 2 Dermabacter hominis strains is mainly attributed to the butanol partition whose major compounds are glycosides of flavones.

Metabolomic Profile and Cytotoxic Activity of Cissus incisa Leaves Extracts

“…The Selectivity Index (SI) was determined only for hepatocellular carcinoma cell lines, since they were the most sensitive of all tested ( Table 2 ). It has been reported that SI values less than 2 can indicate toxicity for an extract or a pure compound towards mammal cells [ 18 ]. CHCl 3 /MeOH extract gave a SI = 2.77 on Hep3B, and SI = 2.21 on HepG2, surpassing the values of the control (Paclitaxel) on the same cell lines (2.41 and 1.24, respectively).…”

Syringin: A Phenylpropanoid Glycoside Compound in Cirsium brevicaule A. GRAY Root Modulates Adipogenesis

“…The genus Cirsium (thistle) belongs to the Asteraceae family; more than 200 species of thistle are distributed around the world. Some Cirsium species are used as edible and medicinal plants in traditional medicine, although other species are often considered as invasive weeds against which massive means of herbicidal control are deployed [ 7 , 8 ]. It is well known that Cirsium species contain a variety of natural products, and their main secondary metabolites include flavonoids and glycosides [ 9 , 10 ].…”

Isolation and Quantitative Analysis of BACE1 Inhibitory Compounds from Cirsium maackii Flower

“…Previously, Jung et al have performed HPLC analysis of C. maackii flower and showed the presence of apigenin (8), luteolin (9), apigenin 7-O-glucuronide (12), and luteolin 5-O-glucoside (11) in its MeOH extract. 8 In the present study, we were able to isolate these compounds together with apigenin 7-O-glucuronide methyl ester (14), tracheloside (5), apigenin 5-O-glucoside (10), and luteolin 7-O-glucuronide (13) from C. maackii flowers. Although the presence of a rare flavonoid, apigenin 5-Oglucoside (10), has been long described by Iwashina et al in Japanese Cirsium species by principal component and UV spectra analysis, this is the first chromatographic separation and quantification of this compound from C. maackii.…”