Antiproliferative and antiangiogenic effect of Amblyomma sculptum (Acari: Ixodidae) crude saliva in endothelial cells in vitro

Sá, Paulo Luiz de; Câmara, Diana Aparecida Dias; Sciani, Juliana Mozer; Porcacchia, Allan Saj; Fonseca, Pâmela Maria Moreira; Mendonça, Ronaldo Zucatelli; Elífio-Esposito, Selene; Simons, Simone Michaela

doi:10.1016/j.biopha.2018.11.107

Cited by 7 publications

(3 citation statements)

References 26 publications

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“…dromedarii SGEs compared to SGEs from the other species. Also, that was higher than the inhibitory effect of saliva from the other genera of Ixodidae in previous studies ( 43 ). According to our findings, after being exposed to Hy.…”

Section: Discussioncontrasting

confidence: 48%

In Vitro Assessment of the Anti-Proliferative and Anti-Viability Effects of Salivary Gland Extracts from Hyalomma ticks (Acari: Ixodidae) on Human Colorectal Cancer Cells

Tavassoli,

Kadivar,

Akhavan

et al. 2024

JAD

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Background: The saliva and salivary glands of ticks possess a wide range of immuno-pharmacologically active molecules that effectively modulate the activity of enzymes, antibodies, and amines that have a role in different biological processes. Derived components from saliva and salivary glands of hard ticks Ixodidae have been characterized as potential natural sources for discovering promising anti-cancer drug candidates. Methods: The anti-cancer activity of salivary gland extracts (SGEs) from Hyalomma anatolicum, Hyalomma dromedarii, Hyalomma marginatum, and Hyalomma schulzei was assessed. MTT assays and flow cytometry were done on the HT-29 colorectal cancer cell line to evaluate the anti-viability and proliferative inhibition. Results: Based on the MTT assay results, the SGEs from Hy. dromedarii had the highest and lowest substantial anti-viability effects on the HT-29 cancer cell and human foreskin fibroblast (HFF) normal cell, respectively. The cytometric assessment revealed a significant increase in the apoptosis and necrosis ratio of the HT-29 cancer cells after treatment with Hy. dromedarii SGEs. Conclusion: The results demonstrated that Hy. dromedarii SGEs have significant anti-proliferative, anti-viability, and apoptotic potential. The result of this study suggests that Hy. dromedarii SGEs is an appropriate candidate for further investigations to identify and purify the mechanisms and molecules involved in the anti-cancer activity of the SGEs.

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Section: Discussioncontrasting

confidence: 48%

In Vitro Assessment of the Anti-Proliferative and Anti-Viability Effects of Salivary Gland Extracts from Hyalomma ticks (Acari: Ixodidae) on Human Colorectal Cancer Cells

Tavassoli,

Kadivar,

Akhavan

et al. 2024

JAD

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“…The data reviewed showed that the presence of ectoparasites, in general, tends to negatively influence the red series and positively influence the white series of the blood count components, probably because of the hematophagous habit of most of the analyzed ectoparasites and the defense response of the host body. This probably happens because the host immune system responds to infestation with ectoparasites, while ectoparasite saliva may contain molecules that act as anti-inflammatory, immunosuppressive, anti-hemostatic, anti-angiogenic, and vasodilators (Jones, 1998; Cavalcante et al ., 2003; Batista et al ., 2010; Rizzo et al ., 2011; Carvalho-Costa et al ., 2015; Esteves et al ., 2017; Moreira et al ., 2017; Sá Junior et al ., 2019).…”

Section: Discussionmentioning

confidence: 99%

Evidence that ectoparasites influence the hematological parameters of the host: a systematic review

Nogueira,

da Silva Soares,

Mauricio Ortega Orozco

et al. 2023

Anim. Health. Res. Rev.

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Ectoparasites are important to the one health concept because their parasitism can result in the transmission of pathogens, allergic reactions, the release of toxins, morbidity, and even death of the host. Ectoparasites can affect host physiology, as reflected in immune defenses and body condition as well as hematological and biochemical parameters. Thus, evidence that ectoparasites influence host hematological parameters was systematically reviewed, and the methodological quality of these studies was analyzed. The Preferred Reporting Items for Systematic Reviews and Meta-Analysis guidelines were followed, and the studies included were limited to those that evaluated changes in hematological tests in ectoparasite-infested and non-infested animals, and bias and methodological quality were evaluated using the Animal Research: Reporting of In Vivo Experiments guideline. Thirty-four studies were selected and information about the host, ectoparasite infestation, blood collection, and analysis was collected and compared whenever possible. In this review, the presence of ectoparasites influenced both the red series and the white series of hematological parameters. Among the main parameters analyzed, hematocrit, red blood cells, hemoglobin, and lymphocytes showed reductions, probably due to ectoparasite blood-feeding, while including eosinophils, neutrophils, and basophils increased in infested animals due to the host immune response. However, methodologic improvements are needed to reduce the risk of bias, enhance the reproducibility of such studies, and ensure results aligned with the mechanisms that act in the ectoparasite-host relationship.

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“…Tick saliva is a mixture of proteins, peptides and non-peptide molecules that interfere with various components of hemostasis, wound healing, and both arms of the immune system of the vertebrate hosts, including enzymes, cytokines, complement, antibodies, cell signaling components, immune cells (Francischetti et al, 2009; Mans, 2016; Nuttall, 2018; Wikel, 2018). In addition, cytotoxic and cytolitic activities acting against different cell types, impairment of cancer cells migration and signaling pathways, as well as anti-angiogenic properties have been demonstrated for saliva of different hard tick species (Kazimirova et al, 2006; Poole et al, 2013; Holíková et al, 2018; Sousa et al, 2018; de Sá Jr. et al, 2019; Gradowski do Nascimento et al, 2019), showing that tick saliva is an important source for designing new anticancer drugs (Kazimírová, 2011; Sousa et al, 2015; Chudzinski-Tavassi et al, 2016). Proteinaceous components of the tick saliva are grouped into families like lipocalins, proteins with Kunitz type domain, metalloproteases, serpins, cystatins, basic-tail secreted proteins, small peptide inhibitors, some protein families unique to ticks, and proteins and peptides of unknown structure and function (Ribeiro et al, 2006; Francischetti et al, 2009; Chmelar et al, 2012).…”

Section: Ticks and Pharmacological Properties Of Their Salivamentioning

confidence: 99%

Deciphering Biological Processes at the Tick-Host Interface Opens New Strategies for Treatment of Human Diseases

et al. 2019

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Ticks are obligatory blood-feeding ectoparasites, causing blood loss and skin damage in their hosts. In addition, ticks also transmit a number of various pathogenic microorganisms that cause serious diseases in humans and animals. Ticks evolved a wide array of salivary bioactive compounds that, upon injection into the host skin, inhibit or modulate host reactions such as hemostasis, inflammation and wound healing. Modulation of the tick attachment site in the host skin involves mainly molecules which affect physiological processes orchestrated by cytokines, chemokines and growth factors. Suppressing host defense reactions is crucial for tick survival and reproduction. Furthermore, pharmacologically active compounds in tick saliva have a promising therapeutic potential for treatment of some human diseases connected with disorders in hemostasis and immune system. These disorders are often associated to alterations in signaling pathways and dysregulation or overexpression of specific cytokines which, in turn, affect mechanisms of angiogenesis, cell motility and cytoskeletal regulation. Moreover, tick salivary molecules were found to exert cytotoxic and cytolytic effects on various tumor cells and have anti-angiogenic properties. Elucidation of the mode of action of tick bioactive molecules on the regulation of cell processes in their mammalian hosts could provide new tools for understanding the complex changes leading to immune disorders and cancer. Tick bioactive molecules may also be exploited as new pharmacological inhibitors of the signaling pathways of cytokines and thus help alleviate patient discomfort and increase patient survival. We review the current knowledge about tick salivary peptides and proteins that have been identified and functionally characterized in in vitro and/or in vivo models and their therapeutic perspective.

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Antiproliferative and antiangiogenic effect of Amblyomma sculptum (Acari: Ixodidae) crude saliva in endothelial cells in vitro

Cited by 7 publications

References 26 publications

In Vitro Assessment of the Anti-Proliferative and Anti-Viability Effects of Salivary Gland Extracts from Hyalomma ticks (Acari: Ixodidae) on Human Colorectal Cancer Cells

In Vitro Assessment of the Anti-Proliferative and Anti-Viability Effects of Salivary Gland Extracts from Hyalomma ticks (Acari: Ixodidae) on Human Colorectal Cancer Cells

Evidence that ectoparasites influence the hematological parameters of the host: a systematic review

Deciphering Biological Processes at the Tick-Host Interface Opens New Strategies for Treatment of Human Diseases

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