Antiproliferative Activity of Rhinacanthus nasutus (L.) KURZ Extracts and the Active Moiety, Rhinacanthin C

Gotoh, Akinobu; Sakaeda, Toshiyuki; Kimura, Takashi; Shirakawa, Toshiro; Wada, Yoshitaka; Wada, Atsushi; Kimachi, Tetsutaro; Takemoto, Yoshiji; Iida, Akira; Iwakawa, Seigo; Hirai, Midori; Tomita, Hideyuki; Okamura, Noboru; Nakamura, Tsutomu; Okumura, Katsuhiko

doi:10.1248/bpb.27.1070

Cited by 49 publications

(37 citation statements)

References 11 publications

(13 reference statements)

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“…Active compounds in RN leaves were widely investigated. The major compounds were naphthoquinone including rhinacanthin (A-D, G-Q), rhinacanthone and lignan group (Gotoh et al, 2004;Panichayupakaranant et al, 2009;Wu et al, 1998).…”

Section: Disscussionmentioning

confidence: 99%

Anti-oxidant properties and anti-hemolytic activity of Psidium guajava, Pandanous odorus and Rhinacanthus nasutus

Thephinlap¹,

Pangjit²,

Suttajit³

et al. 2013

J. Med. Plants Res.

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The ethanolic extracts of leaves of Psidium guajava (PG), Pandanous odorus (PO), Rhinacanthus nasutus (RN) contained polyphenolic compounds in the order of PG > RN > PO. All the extracts exhibited 2,2-diphenyl-1-picryhydrazyl (DPPH) and 2,2'-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radicals scavenging activities. PG showed a marked antioxidant effect whereas PO and RN were comparable. Interestingly, they were able to bind with ferric (Fe 3+ ) and ferrous (Fe 2+) ion and completed within 5 min of incubation times. In accordance with previous results, PG extract could effectively protect erythrocytes from iron induced hemolysis with the activity higher than PO and RN. Based on all results, tested leaf extracts of three Thai medicinal plants might be used for alleviating or preventing harmfulness in many oxidative stress disorders.

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Section: Disscussionmentioning

confidence: 99%

Anti-oxidant properties and anti-hemolytic activity of Psidium guajava, Pandanous odorus and Rhinacanthus nasutus

Thephinlap¹,

Pangjit²,

Suttajit³

et al. 2013

J. Med. Plants Res.

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“…[2][3][4][5][6][7][8][9][10][11][12] The main bioactive components of the plant are known to be naphthoquinones such as rhinacanthins (A-D, G-Q), rhinacanthone and lignan groups.…”

Section: )mentioning

confidence: 99%

“…[2][3][4][5][6][7][8][9][10][11][12] The main bioactive components of the plant are known to be naphthoquinones such as rhinacanthins (A-D, G-Q), rhinacanthone and lignan groups. [3][4][5][6][7][8][9][10][11][12] Naphthoquinone compounds have been reported to possess antiproliferative activity against a panel of cancer cells.…”

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confidence: 99%

Induction of Apoptosis in Tumor Cells by Three Naphthoquinone Esters Isolated from Thai Medicinal Plant: Rhinacanthus nasutus KURZ.

Siripong

Yahuafai

Shimizu

et al. 2006

Biological & Pharmaceutical Bulletin

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Cancer causes significant morbidity and mortality and is a major public problem world widely. Cancer chemotherapy, a routine method for the treatment in cancer patients, faces on the problems of unstable efficiency, severe side effects, and cost. Therefore, new drugs with high efficacy without severe side effects, along with understanding the molecular basis in cancer treatment, are awaited.Many attentions have been focused on natural products as potential sources of novel anticancer drugs over the decades. 1)Rhinacanthus nasutus KURZ. (family Acanthaceae), has been used as Thai traditional medicine for the treatment of various cancers, e.g. cervical and liver cancers.2) Various parts of this plant have been also used for the treatment in various other diseases such as eczema, pulmonary tuberculosis, herpes, hepatitis, diabetes, hypertension, and various skin diseases, and the active components of this plant have been widely investigated. [2][3][4][5][6][7][8][9][10][11][12] The main bioactive components of the plant are known to be naphthoquinones such as rhinacanthins (A-D, G-Q), rhinacanthone and lignan groups.3-12) Naphthoquinone compounds have been reported to possess antiproliferative activity against a panel of cancer cells.3,9-12) Recently, our group reported that synthesized compounds of 1,2-naphthoquinones and 1,4-naphthoquinones as well as rhinacanthins-M, -N and -Q, and related naphthoquinone esters showed selective antiproliferative activity against human cancer cells (KB, HeLa and HepG2) with moderate cytotoxicity against non-tumorigenic Vero cells. 13,14) Regarding to the antitumor effects, it was reported that ethanolic and aqueous extracts of the roots and leaves of R. nasutus KURZ. suppressed the growth of ascites sarcoma 180 in Swiss mice, and that rhinacanthin-C was expected as the bioactive compound.11) Moreover, the antitumor activity of rhinacanthone against Dalton's ascites lymphoma was also observed. 12)However, there was no report elucidating the action targets and anticancer mechanism of these potential natural compounds, except one report that both synthesized rhinacanthins-N and -Q could inhibit DNA topoisomerase II, which is known to play a key role in the regulation of cells growth. 14)By the way, it is well recognized that many of available chemotherapeutic drugs including topoisomerase inhibitors mediate their anticancer effects via apoptosis induction in cancer cells.15) Moreover, many drugs derived from natural products such as etoposide, camptothecin, b-lapachone, and so on are known to induce apoptosis. [16][17][18][19][20][21][22][23][24][25][26][27] Especially, b-lapachone, a naturally produced from naphthoquinone compound having similar basic structure to rhinacanthone, showed potent inhibition of DNA topoisomerase I and II, 16,17) and induced apoptosis in various cancer cells. [25][26][27][28][29][30][31] Therefore, it is possible that rhinacanthins would damage tumor cells through the induction of apoptosis. The present study, we investigated the growth inhibitory effect...

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“…The literatures reported that the traditional medicine preparation from roots, stems and leaves of this shrub has long been used in Thai traditional medicine for treatment of various diseases such as eczema, pulmonary tuberculosis, herpes, hepatitis, diabetes, cancer, obesity, leprosy, eczema, scurvy, dhobi's itch hypertension and various skin diseases [3,4]. Several reports of investigation states that leaves and stems of R. nasutus contains various types of flavonoids, benzenoids, coumarin, anthraquinone, quinone, glycosides, carbohydrate, triterpenes, sterols, and chlorophyll [5]. The naphthoquinone; rhinacanthins (A-D, G-Q), rhinacanthone and lignan groups are the main bioactive compounds [6].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Phytochemical and Antibacterial Studies on Rhinacanthus Nasutus (L.) Kurz - A Medicinally Important Plant

Antonysamy¹

2017

JMEN

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The present study was aimed to evaluate and compare the phytochemical and antibacterial potential of mother plants (aerial parts), in vitro leaves and stems derived callus and in vitro callus mediated shootlets of Rhinacanthus nasutus (L.) Kurz. Preliminary phytochemical analysis and extraction was performed on stem and leaves segments derived calli (4 weeks old), aerial portions of mother plants and calli mediated shootlets of R. naustus. Anti-bacterial activity of different extracts (25 µg) (ethanol, chloroform, aqueous and ethyl acetate) of stem and leaves segments derived calli, aerial portions of mother plants and calli mediated shootlets were investigated by well-diffusion method against Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Bacillus subtilis and Escherichia coli. The preliminary phytochemical studies confirmed that the leaves and stem derived calli mediated shootlets showed the higher degree of metabolite constituents and extraction value compared to the in vitro derived calli and in vivo leaves and stem (aerial portions). The ethyl acetate extracted solvents showed the maximum bio-efficacy compared with other solvents due to the presence of more compounds such as saponins, steroids, tannins, phenolics, triterpenoids, alkaloids, coumarins, anthraquinones, glycosides and flavonoids. The present study observation suggested that a possibility to establish high yielding genotypes by in vitro culture for production of medicinally important bioactive compounds. The methods developed in this work make possible for the low volume and high potential production of active principles under in vitro condition in short duration with less amount of explants utilization. .

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Antiproliferative Activity of Rhinacanthus nasutus (L.) KURZ Extracts and the Active Moiety, Rhinacanthin C

Cited by 49 publications

References 11 publications

Anti-oxidant properties and anti-hemolytic activity of Psidium guajava, Pandanous odorus and Rhinacanthus nasutus

Anti-oxidant properties and anti-hemolytic activity of Psidium guajava, Pandanous odorus and Rhinacanthus nasutus

Induction of Apoptosis in Tumor Cells by Three Naphthoquinone Esters Isolated from Thai Medicinal Plant: Rhinacanthus nasutus KURZ.

In Vitro Phytochemical and Antibacterial Studies on Rhinacanthus Nasutus (L.) Kurz - A Medicinally Important Plant

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