Antiproliferative activity of fish protein hydrolysates on human breast cancer cell lines

Picot, Laurent; Bordenave-Juchereau, Stéphanie; Didelot, Sandrine; Fruitier-Arnaudin, Ingrid; Sannier, Frédéric; Þorkelsson, Guðjón; Bergé, Jean‐Pascal; Guérard, Fabienne; Chabeaud, A.; Piot, Jean–Marie

doi:10.1016/j.procbio.2005.11.024

Cited by 194 publications

(113 citation statements)

References 31 publications

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“…The positive control, cyclophosphamide expressed much higher activity of 75.74% at the same concentration. In a study conducted by Picot et al (2006), fish peptides prepared using Protomex and alcalase exhibited 33.3% growth inhibition on HeLa cells, but 81.7% inhibition on HCT-166 cells at 1mg/mL concentration. In an another study, salmon and bluefin tuna skin collagen and collagen peptides exhibited the highest cytotoxicity activity of 65% and 50% at 0.2 mg/mL concentration against HepG2 and HeLa cells, respectively Han et al (2011).…”

Section: In Vitro Anti-cancer Assaymentioning

confidence: 99%

Untitled

Kumar¹,

Shakila²,

Jeyasekaran³

2019

Turk. J. Fish. Aquat. Sci.

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In this study, skin of unicorn leatherjacket (Aluterus monoceros) was hydrolyzed using collagenase at three different temperatures viz. 5°C, 25°C and 50°C, to obtain collagen hydrolysates. Collagen peptides of low molecular masses (<3kDa) viz. CP-5, CP-25 and CP-50 were obtained by sequential ultrafiltration with tangential flow filtration (TFF) system. The results indicated that CP-5 exhibited the maximum in vitro anti-cancer, anti-diabetic and wound healing activities than CP-25 and CP-50. In MTT assay, CP-5 inhibited the viability of COLO320 cancer cells upto 49.78% at 1 mg/mL concentration, while the inhibition was only 29.92% by CP-50. Anti-diabetic activity as assessed by α-amylase inhibition was 80.45% by CP-5 at 1 mg/mL concentration, as against 75.81% and 71.17% by CP-25 and CP-50, respectively. Collagen peptides, in general, exhibited very low anti-inflammatory property, which ranged between 3.27% to 4.01%. In vitro wound healing tested by scratch assay showed good migration of 3T3-L1 cells in CP-5 treated than CP-25 and CP-50 at 0.2 mg/mL concentration. The study thus proved that collagen peptides derived from fish skin at low hydrolysis temperatures (50C) exhibited more bio-active activities than those hydrolysed at high temperatures (500C); and shall be effectively utilized in various bio-medical applications.

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Section: In Vitro Anti-cancer Assaymentioning

confidence: 99%

Untitled

Kumar¹,

Shakila²,

Jeyasekaran³

2019

Turk. J. Fish. Aquat. Sci.

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“…Amino acid sequences of these peptides were LPHVLTPEAGAT from papain hydrolysate and PTAEGVYMVT, from protease XXIII and both of them exhibited dose-dependent antiproliferative activities against human breast adenocarcinoma (MCF-7) cells, (Hsu et al, 2011;Ryan et al, 2011;Udenigwe and Aluko, 2012). Picot et al (2006) reported 18 protein hydrolysates isolated from blue whiting, cod, plaice, and salmon to have antiproliferative activity against 2 human breast cancer cell lines (MCF-7/6 and MDA-MB-231) (Picot et al, 2006;Shahidi and Zhong, 2008;Ryan et al, 2011). In addition, it has be shown that hydrophobic peptide isolated from anchovy sauce, with molecular weight of 440.9 Da, induced apoptosis in a human lymphoma cell line (U937), (Lee et al, 2003;Lee et al, 2004;Ryan et al, 2011).…”

Section: Anticancer Propertiesmentioning

confidence: 99%

Bioactive peptides from meat and their influence on human health

Baltić¹,

Bošković²,

Ivanović³

et al. 2014

Tehnologija mesa

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“…Recent studies have identified and characterized, peptides derived from animal and vegetal sources as promising chemopreventive agents [12][13][14]. One of these peptides, called lunasin, was identified in soybean and other plants and legumes.…”

Section: Chemopreventive Role Of Food Peptidesmentioning

confidence: 99%