Antiproliferative activity of antimicrobial peptides and bioactive compounds from the mangrove Glutamicibacter mysorens

Abstract

The Glutamicibacter group of microbes is known for antibiotic and enzyme production. Antibiotics and enzymes produced by them are important in the control, protection, and treatment of chronic human diseases. In this study, the Glutamicibacter mysorens (G. mysorens) strain MW647910.1 was isolated from mangrove soil in the Mangalore region of India. After optimization of growth conditions for G. mysorens on starch casein agar media, the micromorphology of G. mysorens was found to be spirally coiled spore chain, each spore visualized as an elongated cylindrical hairy appearance with curved edges visualized through Field Emission Scanning Electron Microscopy (FESEM) analysis. The culture phenotype with filamentous mycelia, brown pigmentation, and ash–colored spore production was observed. The intracellular extract of G. mysorens characterized through GCMS analysis detected bioactive compounds reported for pharmacological applications. The majority of bioactive compounds identified in intracellular extract when compared to the NIST library revealed molecular weight ranging below 1kgmole−1. The Sephadex G-10 could result in 10.66 fold purification and eluted peak protein fraction showed significant anticancer activity on the prostate cancer cell line. Liquid Chromatography–Mass Spectrometry (LC–MS) analysis revealed Kinetin-9-ribose and Embinin with a molecular weight below 1 kDa. This study showed small molecular weight bioactive compounds produced from microbial origin possess dual roles, acting as antimicrobial peptides (AMPs) and anticancer peptides (ACPs). Hence, the bioactive compounds produced from microbial origin are a promising source of future therapeutics.

The metabolites of light: Untargeted metabolomic approaches bring new clues to understand light-driven acclimation of intertidal mudflat biofilm

“…The cyclopentaneundecanoic acid (C60) is found in different microoganism's extracts. It represented 0.69% in the diatom Amphora coffeaeformis (Hassan et al, 2021), 22.7% in the bacteria Glutamicibacter mysorens (Karthik et al, 2023), 5.9 % and 11.06 % in the cyanobacteria Lyngbya sp. (Prasannabalaji et al, 2017) and Anabaena sphaerica (Abd El-Aty et al, 2014) respectively.…”

Promising bioactive metabolites of mangrove inhabitant Streptomyces tauricus and prostate cancer PC3 cell inhibition by antimicrobial peptides

“…The produced formazan was then solubilized by adding 100 μL of solubilization solution (DMSO) and gently shaking the plate in a gyrator shaker. The absorbance was measured using a microplate reader of the Multiskan sky ELISA spectrophotometer at a wavelength of 570 nm and also at 630 nm (Karthik et al, 2023).…”

“…The genetic and environmental adaptability of the Actinomyces group supports the production of valuable and biologically important compounds. Among the Actinomyces, the genus Streptomyces shares more than 70 percent of the active compounds with the pharmaceutical industry for the treatment of various ailments (Chater, 2013;Karthik et al, 2023).…”