Antiproliferative Activity and Induction of Apoptosis in Human Melanoma Cells by Houttuynia cordata Thunb Extract

Yanarojana, Mongkol; Nararatwanchai, Thamthiwat; Thairat, Sarut; Tancharoen, Salunya

doi:10.21873/anticanres.12119

Cited by 19 publications

(13 citation statements)

References 16 publications

(21 reference statements)

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“…Interestingly, we obtained melanogenesis and insulin relative pathways, which were consistent with the previous report that extract of H.cordata was involved in human melanoma cells with antiproliferative and pro-apoptosis activity [28], and anti-obesity via suppressing fatty acid uptake [11].…”

Section: Bioinformatics Analysissupporting

confidence: 91%

“…Melanoma is a dangerous skin cancer in the world [33], and with melanogenesis dysfuction, the excessive accumulation of melanin would cause diversified diseases such as malaise and cancer [34]. As shown in the Mongkol's study, H.cordata extract could induce programmed cell death of the malignant melanoma cell line (A375) [28]. And microphthalamia-associated transcription factor(MITF) was involved in the expression and transport of melanosome component proteins [35,36].…”

Section: Discussionmentioning

confidence: 99%

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Identification of microRNA in Houttuynia cordata Thunb and prediction of cross kingdom functions

Chen

et al. 2019

ExRNA

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Houttuynia cordata Thunb (Family: Saururaceae) is well known as a traditional medicine plant and widely spread in China, Japan and India. It had been reported to have potential functions such as anti-bacterial, anti-cancer, and anti-inflammatory in human. Recently, it has been reported that the plant-derived microRNA(miRNA) possibly transported from one species to another and exerted a cross kingdom regulation, and miRNA has been considered as medicinal ingredents in herbs. However, knowledge is still rare about miRNAs in H. cordata. In this study we identified 163 conserved miRNAs and 30 novel miRNAs by high-throughput sequencing, and then randomly selected miRNA’s expression trend was identified by q-PCR, which was consistent with the sequencing result. Further bioinformatics analyses showed that the targets of H. cordata miRNAs were enriched in endocrine and other factor-regulated calcium reabsorption pathways, melanogenesis, insulin signaling pathway, and aldosterone -regulated sodium reabsorption pathways. These results will be helpful to understand new active components in H. cordata as food and traditional Chinese medicine. This study is the first report of miRNAs in H. cordata, and provides valuable data for further understanding the cross kingdom function of active components in H.cordata.

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Section: Bioinformatics Analysissupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Identification of microRNA in Houttuynia cordata Thunb and prediction of cross kingdom functions

Chen

et al. 2019

ExRNA

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“…Increased expression of HMGB1 is known to be correlated with the progression of human cutaneous melanoma and poor patient survival. Studies have confirmed that a member of the Saururaceae family, Houttuynia cordata Thunb (HCT), can induce the activation of caspase-8 and caspase-3, activate p38, and MAPK proteins to induce apoptosis, and inhibit the release of HMGB1 46 . Above all, pyroptosis has an obvious therapeutic effect in melanoma cells.…”

Section: Assosiation Between Caspase-3 Dependent Cell Death and Cancementioning

confidence: 99%

The caspase-3/GSDME signal pathway as a switch between apoptosis and pyroptosis in cancer

et al. 2020

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Apoptosis has long been recognized as a mechanism that kills the cancer cells by cytotoxic drugs. In recent years, studies have proved that pyroptosis can also shrink tumors and inhibit cells proliferation. Both apoptosis and pyroptosis are caspase-dependent programmed cell death pathways. Cysteinyl aspartate specific proteinase-3 (Caspase-3) is a common key protein in the apoptosis and pyroptosis pathways, and when activated, the expression level of tumor suppressor gene Gasdermin E (GSDME) determines the mechanism of tumor cell death. When GSDME is highly expressed, the active caspase-3 cuts it and releases the N-terminal domain to punch holes in the cell membrane, resulting in cell swelling, rupture, and death. When the expression of GSDME is low, it will lead to the classical mechanism of tumor cell death, which is apoptosis. More interestingly, researchers have found that GSDME can also be located upstream of caspase-3, connecting extrinsic, and intrinsic apoptotic pathways. Then, promoting caspase-3 activation, and forming a self-amplifying feed-forward loop. GSDME-mediated pyroptosis is correlated with the side effects of chemotherapy and anti-tumor immunity. This article mainly reviews the caspase-3/GSDME signal pathway as a switch between apoptosis and pyroptosis in cancer, to provide new strategies and targets for cancer treatment.

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“…Ki67 is a nuclear protein that is expressed in proliferating cells and is a recognized marker for cell proliferation in solid tumors 22 . Caspase-3 is an apoptosis marker of cells undergoing apoptosis 23 and the immunohistochemistry protocol used in this study labels cells with activated caspase-3 presence. Briefly, histological melanoma sections (3–4 µm) of control and EgKI-1-treated mice were affixed to positively charged adhesive slides and air-dried overnight at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Kunitz type protease inhibitor from the canine tapeworm as a potential therapeutic for melanoma

Ranasinghe

Rivera

Boyle

et al. 2019

Sci Rep

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Modulating the tumor microenvironment to promote an effective immune response is critical in managing any type of tumor. Melanoma is an aggressive skin cancer and the incidence rate is increasing worldwide. Potent protease inhibitors have recently been extensively researched as potential therapeutic agents against various cancers. EgKI-1 is a potent Kunitz type protease inhibitor identified from the canine tapeworm Echinococcus granulosus that has shown anti-cancer activities in vivo. In this study we show that EgKI-1 significantly reduced the growth of melanoma in the B16-F0 mouse model and was not toxic to normal surrounding tissue. Moreover, EgKI-1 treatment significantly reduced survivin expression levels and increased the CD8+ T cell population in draining axillary lymph nodes. Therefore, EgKI-1 potentially reduces tumor growth by inducing apoptosis and modulating the tumor microenvironment, and has potential for development as an intra-lesional treatment for melanoma.

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Antiproliferative Activity and Induction of Apoptosis in Human Melanoma Cells by Houttuynia cordata Thunb Extract

Abstract: Abstract. Background

Cited by 19 publications

References 16 publications

Identification of microRNA in Houttuynia cordata Thunb and prediction of cross kingdom functions

Identification of microRNA in Houttuynia cordata Thunb and prediction of cross kingdom functions

The caspase-3/GSDME signal pathway as a switch between apoptosis and pyroptosis in cancer

Kunitz type protease inhibitor from the canine tapeworm as a potential therapeutic for melanoma

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