Antiplatelet, anticoagulant, and profibrinolytic activities of cudratricusxanthone A

Abstract

Cudratricusxanthone A (CTXA), a natural bioactive compound extracted from the roots of Cudrania tricuspidata Bureau, is known to possess hepatoprotective, antiproliferative and anti-inflammatory activities. However, antiplatelet, anticoagulant, and profibrinolytic properties have not been studied. The anticoagulant activities of CTXA were measured by monitoring activated partial thromboplastin-time (aPTT), prothrombin time (PT), and the activities of cell-based thrombin and activated factor X (FXa). The effects of CTXA on the expressions of plasminogen activator inhibitor type 1 (PAI-1) and tissue-type plasminogen activator (t-PA) were also tested in tumor necrosis factor-α (TNF-α) activated human umbilical vein endothelial cells. Our data showed that CTXA inhibited thrombin-catalyzed fibrin polymerization and platelet aggregation, prolonged aPTT and PT significantly and inhibited the activities and production of thrombin and FXa. CTXA prolonged in vivo bleeding time and inhibited TNF-α induced PAI-1 production. Furthermore, PAI-1/t-PA ratio was significantly decreased by CTXA. Collectively, these results indicate that CTXA possesses antithrombotic activities and suggest that the current study could provide bases for the development of new anticoagulant agents.

Benzylated and prenylated flavonoids from the root barks of Cudrania tricuspidata with pancreatic lipase inhibitory activity

“…Compounds isolated from C. tricuspidata roots can be divided into chromones (1-2), isoflavonoids (3)(4)(5)(6), flavonols (7)(8), flavanonols (9-10) and flavanones (11)(12)(13)(14)(15)(16)(17). Overall, flavanones showed stronger pancreatic lipase inhibition than other groups in our assay system (Table 1).…”

“…1 Previous studies reported the isolation of diverse and xanthones their antitumor and anti-inflammatory activity. [2][3][4][5][6] Obesity is a global health problem and associated with several disorders, including diabetes, hypertension, atherosclerosis and cancer. In modern days, increased fat intake is an important factor of obesity.…”

“…As a result, the CH 2 Cl 2 and EtOAc-soluble fraction of C. tricuspidata significantly inhibited pancreatic lipase activity in our assay system. Further chromatographic separation of these fractions afforded seventeen compounds (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17). 10 Compounds 1-16 were identified as 5,7-dihydroxychromone (1), 5-hydroxy-2,2-dimethyl-2H,6H-benzodipyran-6-one (2), genistein (3), biochanin A (4), 3 0 -O-methylorobol (5), wighteone (6), kaempferol (7), morin (8), aromadendrine (9), (2R,3R)-2,3-dihydro-3,5,6,7-tetrahydroxy-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one (10), naringenin (11), 5,7,3 0 ,5 0 -tetrahydroxyflavanone (12), 8-prenylnaringenin (13), cudraflavanone D (14), cudraflavanone A (15), and 2 0 ,5,7-trihydroxy-4,5 0 -(2,2-dimethylchromeno)-8-(3-hydroxy-3-methylbuthyl)flavanone (16), (Fig.…”

Ameliorative Effect of Vicenin-2 and Scolymoside on TGFBIp-Induced Septic Responses

“…Primary human umbilical vein endothelial cells (HUVECs) were obtained from Cambrex Bio Science (Charles City, IA) and maintained as described previously [7,[15][16][17][18][19][20][21]. Briefly, the cells were cultured to confluency at 37°C and 5 % CO 2 in EBM-2 basal media supplemented with growth supplements (Cambrex Bio Science).…”

Inhibitory effects of polyozellin from Polyozellus multiplex on HMGB1-mediated septic responses

“…Primary HUVECs were obtained from Cambrex Bio Science (Charles City, IA) and maintained as described in previous studies [25,[28][29][30][31][32][33][34][35]. Briefly, the cells were cultured to confluency at 37°C and 5 % CO 2 in EBM-2 (endothelial basal medium) supplemented with growth supplements (Cambrex Bio Science).…”