Antiplatelet Activity ofMorus albaLeaves Extract, Mediated via Inhibiting Granule Secretion and Blocking the Phosphorylation of Extracellular-Signal-Regulated Kinase and Akt

Abstract

Ethnopharmacological Relevance. Morus alba L. leaves (MAE) have been used in fork medicine for the treatment of beriberi, edema, diabetes, hypertension, and atherosclerosis. However, underlying mechanism of MAE on cardiovascular protection remains to be elucidated. Therefore, we investigated whether MAE affect platelet aggregation and thrombosis. Materials and Methods. The anti-platelet activity of MAE was studied using rat platelets. The extent of anti-platelet activity of MAE was assayed in collagen-induced platelet aggregation. ATP and serotonin release was carried out. The activation of integrin α IIb β 3 and phosphorylation of signaling molecules, including MAPK and Akt, were investigated with cytofluorometer and immunoblotting, respectively. The thrombus formation in vivo was also evaluated in arteriovenous shunt model of rats. Results. HPLC chromatographic analysis revealed that MAE contained rutin and isoquercetin. MAE dose-dependently inhibited collagen-induced platelet aggregation. MAE also attenuated serotonin secretion and thromboxane A2 formation. In addition, the extract in vivo activity showed that MAE at 100, 200, and 400 mg/kg significantly and dose-dependently attenuated thrombus formation in rat arterio-venous shunt model by 52.3% (P < 0.001), 28.3% (P < 0.01), and 19.1% (P < 0.05), respectively. Conclusions. MAE inhibit platelet activation, TXB2 formation, serotonin secretion, aggregation, and thrombus formation. The plant extract could be considered as a candidate to anti-platelet and antithrombotic agent.

Genetic diversity among mulberry genotypes from seven countries

“…Additionally, mulberry has both the anticancer and the anti-inflammatory properties, which act through different pathways (Kollar et al 2013;Eo et al 2014;Kwon et al 2015;Qian et al 2015). Some reports have demonstrated the leaf's role in ameliorating obesity-induced reactions, increasing bone density and antiplatelet activity (Ann et al 2015;Sungkamanee et al 2014;Kim et al 2014). Therefore, it is essential and beneficial for us to improve the production and quality of mulberry.…”

Medicinal Plants with Antiplatelet Activity

“…In addition, these results are associated with reduced [Ca 2+ ] i , ATP and serotonin secretion, TXA 2 formation, and integrin αIIbβ3 activation. Moreover, M. alba extract significantly inhibited PI3‐K and Akt phosphorylation (Kim et al ., ). In addition, morusinol, a flavonoid derived from M. alba root bark, significantly inhibited collagen‐induced or AA‐induced platelet aggregation and TXB 2 formation in cultured platelets in a concentration‐dependent manner (Lee et al ., 2012b).…”

Bioactive peptides and proteins as alternative antiplatelet drugs

“…The overall effect was species‐dependent, with kistrin being up to 1.6‐fold more efficient compared to flavoridin, in inhibiting of ADP‐enhanced platelet aggregation in both dogs and buffalo. In contrast, flavoridin exhibited 2.1‐fold stronger effects than kistrin while inhibiting the binding of platelets from horses . Arg‐Gly‐Asp (or RGD), the cell recognition signal, was found to potently inhibit the aggregation induced by ADP in both human and canine platelet‐rich plasma.…”

“…In contrast, flavoridin exhibited 2.1-fold stronger effects than kistrin while inhibiting the binding of platelets from horses. 66 Arg-Gly-Asp (or RGD), the cell recognition signal, was found to potently inhibit the aggregation induced by ADP in both human and canine platelet-rich plasma.…”

“…Diverse natural novel proteins and peptides which affect cardiovascular physiology can be used in controlling various pathological events resultant of platelet aggregation and clotting disorders [66][67][68][69][70] (Table 1). All these pathologies are studied in same experiments, as they are interrelated and a ligand affecting one parameter also influences others, to an extent providing synergistic action.…”