Antiplasmodial, Hepatic and Nephritic Effects of Fractions of Methanol Leaf Extract of Glyphaea brevis in Plasmodium berghei-Infected Mice

Abstract

This study evaluated the antiplasmodial, hepatic and nephritic effects of fractions of Glyphaea brevis methanol leaf extract in P. berghei infected mice. Mice weighing between 15-30 g were infested intraperitoneally with 0.2ml plasmodium infected blood and left for 3 hours before treatment. Infected test groups were treated via oral route of administration with varying doses (200, 300 and 400 mg/kg body weight) of ethylacetate, N-butanol and residual aqueous portion fractions of the Glyphaea brevis methanol extract and Artemisinin (5 mg/kg b.wt) for four days. N-butanol fraction showed the highest antiplasmodial activity (76.64%), followed by residual aqueous portion (73.25%) and ethylacetate (72.99%); Artemisinin has 86.13%. Serum bilirubin (total and conjugated) concentrations of the untreated group (0.82 ± 0.20, 0.51 ± 0.12) were significantly lower (P<0.05) than those in the infected group treated with 300 mg/kg of the residual aqueous portion (1.36 ± 0.20, 0.76 ± 0.05) respectively. Serum albumin levels showed significant (P<0.05) increase in all the groups treated compared to the positive control. Serum total protein, urea and creatinine levels of test groups were not significantly (P>0.05) different from the positive control group. Conclusively, Glyphaea brevis has substantial antiplasmodial activity and could provide a lead for new antimalarial drug development.

In Vitro Antimalarial Susceptibility of Plasmodium falciparum and Plasmodium berghei Isolates to Selected Antimalarial Agents, Column Chromatographic Subfractions of Glyphaea brevis Leaves Extract and FTIR and GCNS of SF8

“…Fresh leaves of G. brevis was collected from Irun Akoko, Ondo State and was brought for identification at the herbarium unit of Botany Department, Ahmadu Bello University, Zaria where a voucher number 2634 was obtained and specimen deposited; the air dried and powdered G. brevis leaves extraction procedures have been earlier reported ( Anjuwon et al . 2019 ) to obtain N-butanol fraction of methanol crude extract (NBFx-MExt).…”