Antiphlogistic Effect of Catalase on Experimental Phacoanaphylactic Endophthalmitis

Abstract

Oxygen-derived free radicals generated by polymorphonuclear leukocytes have recently been implicated as important pathogenic mediators of tissue damage in immune complex-mediated acute inflammatory reactions. In order to study the role of oxygen radicals in experimentally induced phacoanaphylactic endophthalmitis, rats were immunized with soluble bovine lens protein and, following surgical disruption of the anterior lens capsule, subjected to treatment with catalase. Our data show that phacoanaphylactic endophthalmitis, as determined morphologically and by measurements of retinal edema, choroidal inflammation, retinal hemorrhage and necrotizing vasculitis, is greatly diminished in catalase-treated animals. This protective effect of catalase indicates that hydrogen peroxide (H₂O₂) and/or conversion products of H₂O₂ (e.g., hydroxyl radical, hypochlorous acid) may play an important role in the development of experimental phacoanaphylactic endophthalmitis.

Anti-Inflammatory Effect of Glutathione Peroxidase on Experimental Lens-Induced Uveitis

“…In the present animal model of lens-induced uveitis, the injury would immediately de stroy the blood-ocular barrier similar to our previous report on catalase [20].…”

Treatment of Experimental Lens-Induced Uveitis by Dimethyl Thiourea

“…It has been demonstrated that antioxi dants effectively reduce inflammation in ex perimental lens-induced uveitis [1][2][3][4][5]. Many immunologically related activities, such as arachidonic acid metabolism, production of chemotactic agents, inactivation of serum protease inhibitors, alterations in collagen and hyaluronic acid, and reduction in 'help er' T-cell subsets, are modulated by antioxi dants [6][7][8][9][10][11][12][13].…”

Suppression of S Antigen-Induced Uveitis by Vitamin E Supplementation

“…Earlier investigations in our laboratory on two models of ocular inflammation, S antigen-and lens-induced uveitis, demon strated that treatment with antioxidant agents such as catalase [ 14], dimethyl sulfox ide [26], superoxide dismutase [27] and de feroxamine mesylate [28] caused a signifi cant decrease in the ocular disease. In other studies, Rehan et al [29] ameliorated acute nephrotoxic nephritis in the rat by using cat alase, while Puig-Parellada and Planas [30] showed that the free radical scavengers man nitol, benzoate and magnesium chloride in hibited synovial fluid degradation in vitro.…”

“…The left eyes from all animals were fixed in 4% buffered formalin and processed for paraffin embedding. The sections were stained with hematoxylin and eosin and one slide from each eye was randomly selected to evaluate the severity of the inflammation using morphometric analysis, as previously described [ 14],…”