Antipeptide activity of intestinal microsyмbyonts for the state of intestinal homeostasis estimation

Бондаренко, Т. А.; Danilova, E. I.; Чайникова, И. Н.; Челпаченко, О. Е.; Иванова, Е. В.; Перунова, Н. Б.; Никифоров, Ю. В.

doi:10.31857/s102872210002385-8

Cited by 2 publications

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“…10 11 Currently, secretory inhibitors of cytokines are being actively studied, that is, the ability of cell-free supernatants of microorganisms to reduce the level of cytokines after their cocultivation. 12 13 The important and paramount role of the immune status at the local level in the pathogenesis of chronic prostatitis has also been noted. 14 However, the available research on the immunomodulatory effect of symbiotic bacteria does not allow the effect of microorganisms on the level of cytokines in semen to be assessed.…”

Section: Introductionmentioning

confidence: 99%

Semen microbiota and cytokines of healthy and infertile men

Бухарин

Перунова

Иванова

et al. 2021

Asian Journal of Andrology

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In this study, we determined the levels of cytokine secretory inhibitors and the microbiota biofilms of semen from healthy and infertile subjects. A total of 118 clinical bacterial isolates were isolated and tested. Cytokine secretory inhibitors were determined based on the difference in cytokine content between the control and experimental samples of cell-free supernatants of isolated microorganisms. Biofilm formation was studied by determining the adhesion of microorganisms to the surface of a 96-well sterile plate and expressed as the optical density at 630 nm (OD 630 ). Cell-free supernatants of Staphylococcus contained higher levels of secretory inhibitor of cytokines in conditionally healthy than in infertile patients. In contrast, in infertile men, the ability to reduce cytokine levels was more characteristic of Enterococcus and Corynebacterium . Seminal Staphylococcus , Corynebacterium , and Enterococcus isolated from infertile subjects showed a greater ability to form biofilms than the same bacteria isolated from healthy men. Further research is needed on this topic, since it is necessary to determine the relationships between decreased secretory inhibitors of cytokines, production of biofilms by bacteria in semen, and infertility. It is likely that the ability of microorganisms to change the concentration of cytokines and increase the level of biofilm formation in semen may be associated with minimal impairments of fertilizing ability, which are not detected using other methods.

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Section: Introductionmentioning

confidence: 99%

Semen microbiota and cytokines of healthy and infertile men

Бухарин

Перунова

Иванова

et al. 2021

Asian Journal of Andrology

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Anticytokine activity of <i>Candida</i> spp. and their ability to produce cytokine-like substances

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Pashkova

Карташова

et al. 2022

Russian Journal of Immunology

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Bacteria are known both to exhibit anticytokine activity (ACA), i.e., to secrete extracellular compounds which inactivate / neutralize various cytokines, and to produce cytokine-like substances (CPV) released to the culture medium. At the same time, the ability of Candida to secrete such substances has not been previously studied. The purpose of our study was to determine the presence of ACA and the ability to produce CPV in Candida strains isolated from the reproductive tract of apparently healthy pregnant women. The experimental series included 26 clinical strains of Candida spp. isolated from vaginal secretions. Isolation and identification of fungal species was based on morphological and biochemical criteria. ACA against IL-4, IL-6, IL-8, IL-10, IL-17A and TNF were detected following 2-hour co-incubation of fungal suspensions with solutions of the distinct cytokines at a ratio of 1:1. Determination of the cytokine concentrations was carried out by ELISA using the Cytokin kits (St. Petersburg). ACA amounts were defined as the percentage of cytokine inactivation in experimental samples compared to the control expressed as pg/mL. Their ability to produce CPV was expressed as percentage of cytokine production in experimental specimens compared to the controls (pg/mL). The obtained data were subjected to statistical evaluation. ACA of Candida spp. and their ability to produce CPV were revealed for the first time. with appropriate differences established between the studied Candida species, i.e., cultures of C. non-albicans showed ACA more often for the pro-inflammatory cytokines; C. albicans isolates showed more frequent production of the substances, similar to the pro-inflammatory cytokines IL-8, IL-17A, and TNF. Expression of ACA against IL-10 and ability to produce the anti-inflammatory IL-4- and IL-10-like substances were significantly higher in the cultures of C. non-albicans species. The results of these experiments expand our knowledge on the spectrum of Candida biological activities and require further study.

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Antipeptide activity of intestinal microsyмbyonts for the state of intestinal homeostasis estimation

Cited by 2 publications

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Semen microbiota and cytokines of healthy and infertile men

Semen microbiota and cytokines of healthy and infertile men

Anticytokine activity of <i>Candida</i> spp. and their ability to produce cytokine-like substances

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