Antioxidative responses in roots and shoots of creeping bentgrass under high temperature: Effects of nitrogen and cytokinin

Wang, Kehua; Zhang, Xunzhong; Ervin, Erik H.

doi:10.1016/j.jplph.2011.12.007

Cited by 49 publications

(42 citation statements)

References 45 publications

(67 reference statements)

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“…He and Huang (2010) detected eight POD isozymes in kentucky bluegrass subjected to heat stress. Wang et al (2012) reported enhanced POD activity and two newly induced POD isoforms in creeping bentgrass under high temperature. In contrast, Liu et al (1995) reported a decline in the intensity of a POD isoform in wheat (Triticum aestivum L.) seedlings in response to heat stress.…”

Section: Discussionmentioning

confidence: 97%

Antioxidant and Hormone Responses to Heat Stress in Two Kentucky Bluegrass Cultivars Contrasting in Heat Tolerance

Li¹,

Zhan²,

Xu³

et al. 2014

J. Amer. Soc. Hort. Sci.

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ADDITIONAL INDEX WORDS. abscisic acid, indole-3-acetic acid, lipid peroxidation, superoxide dismutase, turfgrass ABSTRACT. Heat stress is a major limiting factor for growth of cool-season perennial grass species, and mechanisms of heat tolerance have not been well understood. This study was designed to investigate antioxidant enzyme and hormone metabolism responses to heat stress in two kentucky bluegrass (Poa pratensis L.) cultivars contrasting in heat tolerance. The plants were subjected to 20/20 8C [day/night (control)] or 38/30 8C [day/night (heat stress)] for 28 days in growth chambers. Heat stress increased leaf electrolyte leakage (EL) and malondialdehyde (MDA) with heattolerant cultivar EverGlade exhibiting lower levels of EL and MDA relative to heat-sensitive cultivar Kenblue under heat stress. Superoxide dismutase (SOD) and catalase (CAT) activity increased and then declined during 28 days of heat stress. Peroxidase (POD) and ascorbate peroxidase (APX) activity declined and then increased during heat stress. 'EverGlade' had greater activities of SOD, CAT, POD, and APX relative to 'Kenblue' under heat stress. In addition, 'EverGlade' had two additional SOD isozymes and three additional POD isozymes relative to 'Kenblue' under heat stress. Leaf abscisic acid (ABA) increased in response to heat stress. Leaf indole-3-acetic acid (IAA) increased and then declined during heat stress. 'OverGlade' had higher ABA and IAA content relative to 'Kenblue'. At the end of heat stress, leaf IAA and ABA content were 27.8% and 73% higher in 'EverGlade' relative to 'Kenblue', respectively. The results indicated that antioxidant enzymes and the hormones (ABA and IAA) were associated with kentucky bluegrass heat tolerance. Selection and use of cultivars with higher IAA and ABA content and greater antioxidant enzyme activities may improve kentucky bluegrass growth and quality under heat stress.

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Section: Discussionmentioning

confidence: 97%

Antioxidant and Hormone Responses to Heat Stress in Two Kentucky Bluegrass Cultivars Contrasting in Heat Tolerance

Li¹,

Zhan²,

Xu³

et al. 2014

J. Amer. Soc. Hort. Sci.

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“…For instance, generation of ROS including singlet oxygen ( 1 O 2 ), superoxide radical (O 2- ), hydrogen peroxide (H 2 O 2 ), and hydroxyl radical (OH - ) are thought to be symptoms of cellular injury because of heat stress (Liu and Huang, 2000; Larkindale and Huang, 2004; Wang et al, 2012). Hsfs have been suggested to function as molecular sensors that directly sense and respond to the signals of ROS, which in turn activate the HSP expression and anti-oxidative genes.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional Profiling and Identification of Heat-Responsive Genes in Perennial Ryegrass by RNA-Sequencing

et al. 2017

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Perennial ryegrass (Lolium perenne) is one of the most widely used forage and turf grasses in the world due to its desirable agronomic qualities. However, as a cool-season perennial grass species, high temperature is a major factor limiting its performance in warmer and transition regions. In this study, a de novo transcriptome was generated using a cDNA library constructed from perennial ryegrass leaves subjected to short-term heat stress treatment. Then the expression profiling and identification of perennial ryegrass heat response genes by digital gene expression analyses was performed. The goal of this work was to produce expression profiles of high temperature stress responsive genes in perennial ryegrass leaves and further identify the potentially important candidate genes with altered levels of transcript, such as those genes involved in transcriptional regulation, antioxidant responses, plant hormones and signal transduction, and cellular metabolism. The de novo assembly of perennial ryegrass transcriptome in this study obtained more total and annotated unigenes compared to previously published ones. Many DEGs identified were genes that are known to respond to heat stress in plants, including HSFs, HSPs, and antioxidant related genes. In the meanwhile, we also identified four gene candidates mainly involved in C4 carbon fixation, and one TOR gene. Their exact roles in plant heat stress response need to dissect further. This study would be important by providing the gene resources for improving heat stress tolerance in both perennial ryegrass and other cool-season perennial grass plants.

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“…However, higher N levels stimulates excess shoot growth with a cost of carbohydrate reserve [19], [37], [38], which could account for the lower accumulation of HSPs in the grass under high N at Day 50. It would be worthy to mention that the induction of HSPs could be due to secondary effects of N treatments instead of just increased availability of nitrogen for protein synthesis, such as the oxidative stress of increased metabolism due to higher N rates [71], [72]. Further study would be warranted.…”

Section: Discussionmentioning

confidence: 99%

Heat Shock Proteins in Relation to Heat Stress Tolerance of Creeping Bentgrass at Different N Levels

et al. 2014

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Heat stress is a primary factor causing summer bentgrass decline. Changes in gene expression at the transcriptional and/or translational level are thought to be a fundamental mechanism in plant response to environmental stresses. Heat stress redirects protein synthesis in higher plants and results in stress protein synthesis, particularly heat shock proteins (HSPs). The goal of this work was to analyze the expression pattern of major HSPs in creeping bentgrass (Agrostis stolonifera L.) during different heat stress periods and to study the influence of nitrogen (N) on the HSP expression patterns. A growth chamber study on ‘Penn-A4’ creeping bentgrass subjected to 38/28°C day/night for 50 days, was conducted with four nitrate rates (no N-0, low N-2.5, medium N-7.5, and high N-12.5 kg N ha−1) applied biweekly. Visual turfgrass quality (TQ), normalized difference vegetation index (NDVI), photochemical efficiency of photosystem II (Fv/Fm), shoot electrolyte leakage (ShEL), and root viability (RV) were monitored, along with the expression pattern of HSPs. There was no difference in measured parameters between treatments until week seven, except TQ at week five. At week seven, grass at medium N had better TQ, NDVI, and Fv/Fm accompanied by lower ShEL and higher RV, suggesting a major role in improved heat tolerance. All the investigated HSPs (HSP101, HSP90, HSP70, and sHSPs) were up-regulated by heat stress. Their expression patterns indicated cooperation between different HSPs and their roles in bentgrass thermotolerance. In addition, their production seems to be resource dependent. This study could further improve our understanding about how different N levels affect bentgrass thermotolerance.

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Antioxidative responses in roots and shoots of creeping bentgrass under high temperature: Effects of nitrogen and cytokinin

Cited by 49 publications

References 45 publications

Antioxidant and Hormone Responses to Heat Stress in Two Kentucky Bluegrass Cultivars Contrasting in Heat Tolerance

Antioxidant and Hormone Responses to Heat Stress in Two Kentucky Bluegrass Cultivars Contrasting in Heat Tolerance

Transcriptional Profiling and Identification of Heat-Responsive Genes in Perennial Ryegrass by RNA-Sequencing

Heat Shock Proteins in Relation to Heat Stress Tolerance of Creeping Bentgrass at Different N Levels

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