Antioxidative Properties of Mackerel Scomber japonicus Fed a Diet Fortified with Conjugated Linoleic Acid and Ascidian Halocynthia roretzi Tunic Extract

Abstract

This study was performed to assess the antioxidative properties of lipid from aquacultured mackerel Scomber japonicus fed with a diet fortified with conjugated linoleic acid (CLA) and ascidian Halocynthia roretzi tunic extracts by radical scavenging assay. The fish were separated into squid oil (Control) and 2.5% CLA (CA25) groups during the 8-week feeding period. The reducing power of each sample showed high levels of activity compared with α-tocopherol and butylated-hydroxyanisol (BHA) at 0.2-1.0 mg/mL of lipid. Inhibition of linoleic acid oxidation in samples from Control and CA25 groups showed similar activity after 2 days of incubation at 40 ℃ . The 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide, and hydroxyl radical scavenging activities of CLA and carotenoid-deposited sample (CA25) were higher than those of the Control group. The results indicated that the lipid extracted from the viscera of mackerel showed slightly higher antioxidant activities than that from the muscle.

Recent research on bioactive compounds of Scomber spp. and their health effects

“…(2019) and Park et al . (2011). The biological activities of bioactive compounds from mackerel promote the development of new functional compounds that can be used as nutritional supplements, pharmaceuticals, nutraceuticals and food industries.…”

“…have been reported to contain bioactive compounds in their body. Bioactive compounds, such as peptides (Bashir et al ., 2020), lipids (Park et al ., 2011), amino acids, lecithin (Asaduzzaman & Chun, 2014), pepsin‐solubilised collagen (Asaduzzaman et al ., 2019), glycolipids (Bae & Lim, 2012) and muscle protein hydrolysate (Bashir et al ., 2017; Bashir et al ., 2018; Choi et al ., 2016; Ediriweera et al ., 2019; Matsuda & Kubo, 1988) have been isolated from S . japonicus .…”

“…(2019) and Park et al . (2011), bioactive compounds from S. japonicus possess antioxidant activities with DPPH scavenging activity ranging from 36.0% to 90.63%. Asaduzzaman & Chun (2014) showed that 5.5 mg mL −1 of amino acids and lecithin from S. japonicus exhibit more than 50% DPPH scavenging activity, while Park et al .…”

“…Asaduzzaman & Chun (2014) showed that 5.5 mg mL −1 of amino acids and lecithin from S. japonicus exhibit more than 50% DPPH scavenging activity, while Park et al . (2011) reported that lipids at a concentration of 200 mg mL −1 from the same organism had more than 80% DPPH scavenging activity. Nonetheless, peptides at a concentration of 1 mg mL −1 from S. japonicus showed antioxidant activity, with a DPPH scavenging activity lower than 40%, and SOD‐like activity lower than 30% (Bashir et al ., 2020).…”

“…showed some advantages, such as a simpler structure, that provide more stability in different situations and virtually no risk for hazardous immunogenic reactions. They present not only antioxidant activity, but also nutritional and functional properties (Park et al ., 2011; Asaduzzaman & Chun, 2014; Choi et al ., 2016; Bashir et al ., 2017, 2018, 2020; Asaduzzaman et al ., 2019; Ediriweera et al ., 2019). Thus, they can be applied in various industries.…”