Antioxidative Potential of a Streptomyces sp. MUM292 Isolated from Mangrove Soil

Abstract

Mangrove derived microorganisms constitute a rich bioresource for bioprospecting of bioactive natural products. This study explored the antioxidant potentials of Streptomyces bacteria derived from mangrove soil. Based on 16S rRNA phylogenetic analysis, strain MUM292 was identified as the genus Streptomyces. Strain MUM292 showed the highest 16S rRNA gene sequence similarity of 99.54% with S. griseoruber NBRC12873T. Furthermore, strain MUM292 was also characterized and showed phenotypic characteristics consistent with Streptomyces bacteria. Fermentation and extraction were performed to obtain the MUM292 extract containing the secondary metabolites of strain MUM292. The extract displayed promising antioxidant activities, including DPPH, ABTS, and superoxide radical scavenging and also metal-chelating activities. The process of lipid peroxidation in lipid-rich product was also retarded by MUM292 extract and resulted in reduced MDA production. The potential bioactive constituents of MUM292 extract were investigated using GC-MS and preliminary detection showed the presence of pyrazine, pyrrole, cyclic dipeptides, and phenolic compound in MUM292 extract. This work demonstrates that Streptomyces MUM292 can be a potential antioxidant resource for food and pharmaceutical industries.

Investigating the antioxidant potential of Streptomyces sp. MUSC 11 from mangrove soil in Malaysia

“…It has been suggested that understudied ecosystem hold Streptomyces species which can meet the growing demand of drug discovery and development industry [77] . Researchers who made an effort to study Streptomyces from less explored ecosystems such as mangrove forest were able to discover novel Streptomyces species and Streptomyces strains showing potent antioxidant activities [40,42,66,68,78,79] . dards for potential match.…”

A Review on Mangrove Actinobacterial Diversity: The Roles of Streptomyces and Novel Species Discovery

“…Similarly, mangroves have been regarded as one of the rich resources for isolating Streptomyces bacteria, which also includes novel Streptomyces species [76] . There has been increasing number of mangrove-derived Streptomyces strains exhibiting potent bioactivities such as antimicrobial, anticancer, and antioxidant [20,[77][78][79][80][81] . It is possible that mangrove streptomycetes have unique metabolic capacity on the synthesis of bioactive secondary metabolites.…”

The Bioprospecting of Anti-Vibrio Streptomyces species: Prevalence and Applications

“…The interest on the discovery of bioactive compounds from microbial origin is increasingly attractive towards the researchers, especially from the extreme environments. This is because that the sea and soil microbiota are frequently exposed to the complex, fluctuating and competitive environments which is believed to be the driving forces for metabolic pathway adaptation and lead to production of valuable metabolites [35][36][37][38][39] . The extremely diverse and unsurpassed richness of the secondary metabolism exhibited by Streptomyces has made these filamentous bacteria to serve as a rich bioresource for valuable bioactive compounds [35,[40][41][42][43] .…”