Antioxidative photochemoprotector effects of cerium oxide nanoparticles on UVB irradiated fibroblast cells

Peloi, Karen Elaine; Lancheros, César A. Contreras; Nakamura, Celso V.; Singh, Sushant; Neal, Craig J.; Sakthivel, T.; Seal, S.; Silva, Sueli de Oliveira

doi:10.1016/j.colsurfb.2020.111013

Cited by 18 publications

(27 citation statements)

References 45 publications

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“…These effects were further evidenced as potentiated antioxidant activity, such as SOD and GSH, through chemical assay ( Singh et al, 2016 ). Peloi et al (2020) demonstrated that CNPs also prevent UVB-mediated fibroblast cell oxidative damage by decreasing ROS level and increasing antioxidant enzymes activities. Other studies into nanoceria’s effect on oxidative stress conditions have evidenced changes to glutathione and SOD2 expression.…”

Section: Discussionmentioning

confidence: 97%

“…Several studies support CNPs cytoprotective efficacy; demonstrating that different CNP formulations reduce chronic inflammation, promote angiogenesis, promote tissue regeneration, decrease cell death, and increase cell survival in model biological systems (Chigurupati et al, 2012;Das et al, 2014). Our group recently demonstrated that CNP also prevents UVB-induced fibroblast oxidative damage by decreasing reactive oxygen species (ROS) level and increasing antioxidant enzymes activities (Peloi et al, 2020). Cerium exists in a mixed valence state (Ce 3+ and Ce 4+ ) as an oxide and, due to the relatively low redox potential between these states, allows CNPs to have self-regenerative redox cycling properties, upon release or uptake of oxygen (termed oxygen buffering or oxygen storage capacity).…”

Section: Introductionmentioning

confidence: 96%

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Ceria Nanoparticles Decrease UVA-Induced Fibroblast Death Through Cell Redox Regulation Leading to Cell Survival, Migration and Proliferation

Ribeiro

Oliveira

Singh

et al. 2020

Front. Bioeng. Biotechnol.

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Exposure to ultraviolet radiation is a major contributor to premature skin aging and carcinogenesis, which is mainly driven by overproduction of reactive oxygen species (ROS). There is growing interest for research on new strategies that address photoaging prevention, such as the use of nanomaterials. Cerium oxide nanoparticles (nanoceria) show enzyme-like activity in scavenging ROS. Herein, our goal was to study whether under ultraviolet A rays (UVA)-induced oxidative redox imbalance, a low dose of nanoceria induces protective effects on cell survival, migration, and proliferation. Fibroblasts cells (L929) were pretreated with nanoceria (100 nM) and exposed to UVA radiation. Pretreatment of cells with nanoceria showed negligible cytotoxicity and protected cells from UVA-induced death. Nanoceria also inhibited ROS production immediately after irradiation and for up to 48 h and restored the superoxide dismutase (SOD) activity and GSH level. Additionally, the nanoceria pretreatment prevented apoptosis by decreasing Caspase 3/7 levels and the loss of mitochondrial membrane potential. Nanoceria significantly improved the cell survival migration and increased proliferation, over a 5 days period, as compared with UVA-irradiated cells, in wound healing assay. Furthermore, it was observed that nanoceria decreased cellular aging and ERK 1/2 phosphorylation. Our study suggests that nanoceria might be a potential ally to endogenous, antioxidant enzymes, and enhancing the redox potentials to fight against UVA-induced photodamage and consequently modulating the cells survival, migration, and proliferation.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 96%

Ceria Nanoparticles Decrease UVA-Induced Fibroblast Death Through Cell Redox Regulation Leading to Cell Survival, Migration and Proliferation

Ribeiro

Oliveira

Singh

et al. 2020

Front. Bioeng. Biotechnol.

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“…However, increased ROS levels may lead to creating oxidative stress and consequent pathogenic conditions [ 61 ]. To date, various antioxidant agents have been applied to manage ROS levels; nanoceria is among the most promising candidates in soft tissue applications [ 16 , 62 ]. CeO 2 nanoparticles showed excellent antioxidant enzyme-mimetic activity and the potential of scavenging not only ROS (e.g., superoxide radical anion (O 2 •− )) but, also, reactive nitrogen species (RNS) (e.g., nitric oxide radical ( • NO)) [ 63 ].…”

Section: Nanoceria: Biological Superiorities For Soft-tissue Healimentioning

confidence: 99%

“…From the tissue engineering point of view, CNPs offer prominent biological features for accelerating tissue repair and regeneration, including antioxidant, anti-inflammatory, antibacterial, angiogenic, and antiapoptotic activities [ 16 , 17 , 18 , 19 , 20 ]. There are huge numbers of experimental studies clarifying the therapeutic effectiveness of CNPs, either in bare or functionalized forms for biomedical applications [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Cerium Oxide Nanoparticles (Nanoceria): Hopes in Soft Tissue Engineering

et al. 2020

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Several biocompatible materials have been applied for managing soft tissue lesions; cerium oxide nanoparticles (CNPs, or nanoceria) are among the most promising candidates due to their outstanding properties, including antioxidant, anti-inflammatory, antibacterial, and angiogenic activities. Much attention should be paid to the physical properties of nanoceria, since most of its biological characteristics are directly determined by some of these relevant parameters, including the particle size and shape. Nanoceria, either in bare or functionalized forms, showed the excellent capability of accelerating the healing process of both acute and chronic wounds. The skin, heart, nervous system, and ophthalmic tissues are the main targets of nanoceria-based therapies, and the other soft tissues may also be evaluated in upcoming experimental studies. For the repair and regeneration of soft tissue damage and defects, nanoceria-incorporated film, hydrogel, and nanofibrous scaffolds have been proven to be highly suitable replacements with satisfactory outcomes. Still, some concerns have remained regarding the long-term effects of nanoceria administration for human tissues and organs, such as its clearance from the vital organs. Moreover, looking at the future, it seems necessary to design and develop three-dimensional (3D) printed scaffolds containing nanoceria for possible use in the concepts of personalized medicine.

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“…However, we believe that even at low doses of UV‐B radiation, the oxidative metabolic burst of neutrophils can be activated and contribute to pathophysiology of skin damage. Thus, the use of an antioxidant compound that could mediate and avoid the outcomes of ROS production in neutrophils' uncontrolled activation might be one option to deal with the UV‐B overexposure and chronic skin related damage 9,10 …”

Section: Introductionmentioning

confidence: 99%

Engineered nanoceria modulate neutrophil oxidative response to low doses of UV‐B radiation through the inhibition of reactive oxygen species production

Peloi

Ratti

Nakamura

et al. 2021

J Biomedical Materials Res

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To avoid aging and ultraviolet mediated skin disease the cell repair machinery must work properly. Neutrophils, also known as polymorphonuclear leukocytes, are the first and most abundant cell types which infiltrate sites of irradiation and play an important role in restoring the microenvironment homeostasis. However, the infiltration of neutrophils in ultraviolet‐B (UV‐B) irradiated skin might also contribute to the pathophysiology of skin disease. The polymorphonuclear leukocytes activation induced by UV‐B exposure may lead to prolonged, sustained NADPH oxidase activation followed by an increase in reactive oxygen species (ROS) production. Our previous work showed that cerium oxide nanoparticles can protect L929 fibroblasts from ultraviolet‐B induced damage. Herein, we further our investigation of engineered cerium oxide nanoparticles (CNP) in conferring radiation protection specifically in modulation of neutrophils' oxidative response under low dose of UV‐B radiation. Our data showed that even low doses of UV‐B radiation activate neutrophils' oxidative response and that the antioxidant, ROS‐sensitive redox activities of engineered CNPs are able to inhibit the effects of NADPH oxidase activation while conferring catalase and superoxide dismutase mimetic activity. Further, our investigations revealed similar levels of total ROS scavenging for both CNP formulations, despite substantial differences in cerium redox states and specific enzyme‐mimetic reaction activity. We therefore determine that CNP activity in mitigating the effects of neutrophils' oxidative response, through the decrease of ROS and of cell damage such as chromatin condensation, suggests potential utility as a radio‐protectant/therapeutic against UV‐B damage.

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Antioxidative photochemoprotector effects of cerium oxide nanoparticles on UVB irradiated fibroblast cells

Cited by 18 publications

References 45 publications

Ceria Nanoparticles Decrease UVA-Induced Fibroblast Death Through Cell Redox Regulation Leading to Cell Survival, Migration and Proliferation

Ceria Nanoparticles Decrease UVA-Induced Fibroblast Death Through Cell Redox Regulation Leading to Cell Survival, Migration and Proliferation

Cerium Oxide Nanoparticles (Nanoceria): Hopes in Soft Tissue Engineering

Engineered nanoceria modulate neutrophil oxidative response to low doses of UV‐B radiation through the inhibition of reactive oxygen species production

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