Antioxidative peptide from milk exhibits antiosteopenic effects through inhibition of oxidative damage and bone-resorbing cytokines in ovariectomized rats

Mada, Sanusi Bello; Reddi, Srinu; Kumar, Naveen; Kumar, Rohit; Kapila, Suman; Kapila, Rajeev; Trivedi, Ritu; Karvande, Anirudha; Ahmad, Naseer

doi:10.1016/j.nut.2017.06.010

Cited by 59 publications

(40 citation statements)

References 56 publications

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“…In a recent animal study, the daily administration of a novel casein‐derived peptide (VLPVPQK) for 8 weeks was responsible for alleviating osteoporosis in ovariectomized rats through the inhibition of oxidative damage (Mada et al, ). The peptide ingestion led to increased bone mineral density, reduced bone resorbing cytokines and other antiosteopenic effects in the osteoporotic rat model by enhancing antioxidant activity.…”

Section: Antioxidant Propertiesmentioning

confidence: 99%

“…The peptide ingestion led to increased bone mineral density, reduced bone resorbing cytokines and other antiosteopenic effects in the osteoporotic rat model by enhancing antioxidant activity. This was evidenced through effects on various oxidative stress biomarkers including reduced serum malondialdehyde level as well as increased serum SOD and CAT levels (Mada et al, ). Daily oral administration of corn gluten meal hydrolyzate to mice led to significant antioxidant benefits that was mostly dose‐dependent and superior to the effects produced by vitamin E (Liu et al, ).…”

Section: Antioxidant Propertiesmentioning

confidence: 99%

“…Although the ability of protein hydrolyzates and bioactive peptides to act as antioxidative agents has been roundly demonstrated during in vitro (Agrawal, Joshi, & Gupta, ; Alashi et al, ; Harnedy et al, ; Lin et al, ; Rahman et al, ; Wong et al, ) and in vivo (Girgih, Alashi et al, ; Liu et al, ; Mada et al, ; Xu, Li et al, ; Zhao et al, ) tests, there is a dearth of human clinical trials replicating the results. Given the challenge often encountered in translating results from animal and chemical‐based assays to clinical outcomes, future work should be designed to include tests in human subjects.…”

Section: Future Research Needsmentioning

confidence: 99%

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Structural and functional properties of food protein-derived antioxidant peptides

2019

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The aim of this work is to provide a timely examination of the structure-activity relationship of antioxidative peptides. The main production approach involves enzymatic hydrolysis of animal and plant proteins to produce protein hydrolyzates, which can be further processed by membrane ultrafiltration into size-based peptide fractions. The hydrolyzates and peptide fractions can also be subjected to separation by column chromatography to obtain pure peptides. Although the structural basis for enhanced antioxidant activity varies, protein hydrolyzates and peptide fractions that contain largely low molecular weight peptides have generally been shown to be potent antioxidants. In addition to having hydrophobic amino acids such as Leu or Val in their N-terminal regions, protein hydrolyzates, and peptides containing the nucleophilic sulfur-containing amino acid residues (Cys and Met), aromatic amino acid residues (Phe, Trp, and Tyr) and/or the imidazole ring-containing His have been generally found to possess strong antioxidant properties. Practical applicationsHigh levels of reactive oxygen species (ROS) in addition to the presence of metal cations can lead to oxidative stress, which promotes reactions that cause destruction of critical cellular biopolymers, such as proteins, lipids, and nucleic acids. Oxidative stress could be due to insufficient levels of natural cellular antioxidants, which enables accumulation of ROS to toxic levels. A proposed approach to ameliorating oxidative stress is the provision of exogenous peptides that can be consumed to complement cellular antioxidants. Food protein-derived peptides consist of amino acids joined by peptides bonds just like glutathione, a very powerful natural cellular antioxidant. Therefore, this review provides a timely summary of the in vitro and in vivo reactions impacted by antioxidant peptides and the postulated mechanisms of action, which could aid development of potent antioxidant agents. The review also serves as a resource material for identifying novel antioxidant peptide sources for the formulation of functional foods and nutraceuticals. K E Y W O R D S antioxidants, FRAP, lipid peroxidation, metal chelation, peptides, protein hydrolyzates, radical scavenging [Correction added on 30 January 2019: this article has been added to the issue after an inadvertent omission.]

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Section: Antioxidant Propertiesmentioning

confidence: 99%

Section: Antioxidant Propertiesmentioning

confidence: 99%

Section: Future Research Needsmentioning

confidence: 99%

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Structural and functional properties of food protein-derived antioxidant peptides

2019

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“…Among them, NAVPITPTL markedly increased osteoblast differentiation via activation of pAkt signaling pathway (Reddi, Shanmugam, Kapila, & Kapila, ). Additionally, VLPVPQK from buffalo casein‐derived antioxidant peptide showed anti‐osteoporotic effects via inhibition of oxidative damage and bone‐resorbing cytokine production in OVX rats (Mada et al, ).…”

Section: Food‐derived Bioactive Peptides: Production and Bone Health mentioning

confidence: 99%

Bone health-promoting bioactive peptides

Ahn

2018

J Food Biochem

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Bioactive peptides, derivatives of proteins, show versatile biological effects and represent potential health-promoting agents as functional food ingredients and/or nutraceuticals. Bone health depends on the balance between bone formation and resorption. When the balance is disrupted, bone diseases such as osteoporosis and fragility fractures may result. Accumulating evidence suggests that peptides derived from endogenous proteins and food proteins enhance bone health.This article reviews the literature on peptides exhibiting bone health-promoting effects. Possible biochemical mechanisms and production of these peptides are briefly discussed.

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“…In particular, milk proteins function as opioid agonists, angiotensin converting enzyme (ACE) inhibitors, antimicrobials, and antithrombotics (Park & Nam, ). Furthermore, a bioactive peptide, VLPVPQK, derived from buffalo casein exhibits antiaging and antiosteopenic effects (Kumar et al., ; Mada et al., ; Mada, Reddi, Kumar, Kapila, & Kapila, ). However, the effects of milk proteins on cellular senescence remain largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Alpha‐Casein and Beta‐Lactoglobulin from Cow Milk Exhibit Antioxidant Activity: A Plausible Link to Antiaging Effects

Kim

Cheon

et al. 2019

Journal of Food Science

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Studies on the discovery and function of antioxidants are consistently being performed because oxidative stress can cause various diseases. Many compounds and natural products have antioxidant activity in vitro; however, it is often difficult to reproduce their effects in vivo. Additionally, methods to measure antioxidant activities in cells are also scarce. Here, we investigated the antioxidant activity of milk proteins by observing the formation of arsenite‐induced stress granules as a tool to evaluate antioxidant activity in cells. Milk proteins not only decreased the formation of stress granules in several cell types but also scavenged 2,2'‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS) radical cations in vitro. In addition, milk proteins inhibited cellular senescence based on an SA‐β‐galactosidase assay, and increased differentiation to myotubes from myoblasts isolated from the skeletal muscles of mouse pups. Taken together, our results demonstrate that milk proteins have an antiaging effect, especially prevention of skeletal muscle loss, through their antioxidant activities. Practical Application Our results provide that antioxidant effects of milk proteins containing α‐caseins, β‐caseins, and β‐lactoglobulin can mitigate aging‐related damage induced by oxidative stress through showing inhibition of cellular senescence and increase of differentiation and maturation of myoblast. Therefore, we suggest that milk proteins could be potent health supplements to prevent aging‐associated diseases, especially sarcopenia.

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Antioxidative peptide from milk exhibits antiosteopenic effects through inhibition of oxidative damage and bone-resorbing cytokines in ovariectomized rats

Cited by 59 publications

References 56 publications

Structural and functional properties of food protein-derived antioxidant peptides

Structural and functional properties of food protein-derived antioxidant peptides

Bone health-promoting bioactive peptides

Alpha‐Casein and Beta‐Lactoglobulin from Cow Milk Exhibit Antioxidant Activity: A Plausible Link to Antiaging Effects

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