Antioxidative enzymes in irradiated rat brain—indicators of different regional radiosensitivity

Todorović, Ana; Pejić, Snežana; Stojiljković, Vesna; Gavrilović, Ljubica; Popović, Nataša; Pavlović, Ivan; Saičić, Zorica S.; Pajović, Snežana B.

doi:10.1007/s00381-015-2807-2

Cited by 5 publications

(5 citation statements)

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“…In the same line, the existence of cognitive decline in patients after a clinical high dose of irradiation exposure has led to a research emphasis on the hippocampal microenvironment and its population of mature cells and proliferating progenitor cells in the dentate gyrus subgranular zone. This neurogenesis region is particularly ionizing radiation sensitive (rola et al, 2008), as mitotic cells are radiosensitive at the cell cyclestages between the G2 and m phase (marples et al, 2003 andTodorovic et al, 2015). These findings are in agreement with other reports demonstrating that radiation stimulates a premature aging process in the brain and accelerates and/or aggravates the chronic degenerative disorders feature onset for elderly (Dietrich et al, 2008 andhua et al, 2012).…”

Section: Histopathological Examinationsupporting

confidence: 87%

Ameliorative role of Teucrium polium extract on γ-radiation toxicity in brain of albino rats

2019

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T hE ImpaIrmENT to the healthy brain tissue is a crucial factor limiting the application of radiation therapy in patients with nervous system neoplasms. Teucrium polium (Tp) L. (family Lamiaceae), a wild-growing flowering plant, has beneficial curative properties. The flavonoids of TP have many valuable biological activities. This study aims atinvestigating the ameliorative effect of TP extract on γ-radiation toxicity in brain of albino rats. Thirty adult male albino rats were used. Ionizing irradiation was performed by whole body exposure of rats to 8Gy. Tp was administered by intragastric tube in a dose of 200mg/ kg body weight. Animals were divided into five groups: Group I (control), Group II (TP), Group III (γ-irradiated), Group IV (TP+γ-irradiated) and Group V (TP+γ-irradiated+TP). The action of Tp extract was investigated after one week post irradiation by measuring serum liver and renal functions, and oxidative stress parameters in brain tissue. moreover, serum S100B and brain-derived neurotrophic factor (BDNF) in brain tissue were measured, in addition to histopathological examination. Tp at a dose of 200mg/kg body weight did not have hepatotoxic or nephrotoxic effects. TP extract significantly ameliorated γ-radiation-induced brain damage by improving radiationinduced oxidative stress. The role of the extract was confirmed by improvement of the levels of S100B in the serum and BDNF in the brain as well as amelioration of histopathological changes induced by radiation. The administration of Tp before and after irradiation was more effective than administration only before irradiation. These results revealed that the administration of Tp extract might ameliorate γ-radiation-induced brain injury by the attenuation of oxidative stress, the regulation of BDNF and the suppression of S100B.

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Section: Histopathological Examinationsupporting

confidence: 87%

Ameliorative role of Teucrium polium extract on γ-radiation toxicity in brain of albino rats

2019

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“…Sudha et al 27 observed no significant changes of erythrocyte antioxidants in PD patients while Abraham et al 21 reported decreased AO enzymes activity in PD patients compared to controls. Considering that CAT is crucial in removing H 2 O 2 at higher concentrations 35 (GPx is predominant at physiologically low levels of H 2 O 2 36 ), elevation of CAT activity in the blood of PD patients confirms the general conclusion of this study that PD patients are exposed to chronic oxidative stress 37 .…”

Section: Discussionsupporting

confidence: 85%

Antioxidant status and clinicopathological parameters in patients with Parkinson's disease

et al. 2020

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Backgroun/Aim. Constant production of free radicals and antioxidants (AO) in cells is a part of normal cellular function. Their imbalance might take a part in pathophysiology of many diseases, including Parkinson's disease (PD). Evaluation of the disease status, prooxidant-antioxidant balance (PAB) and antioxidants are being widely estimated. The aim of this study was to examine potential interaction between several AO variables: glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and PAB, and clinicopathologic features of patients with PD, particularly the Hoehn and Yahr (H&Y) stage. Methods. A multivariate analysis of variance (MANOVA) was conducted to analyze mean differences between clinicopathologic characteristics (gender, age at examination, duration of the disease, and the H&Y stage) and AO variables of PD patients and those of age/sex matched healthy controls. The study included 91 patients with idiopatic PD patients and 20 healthy persons. Results. The multivariate effect size was estimated at 0.269 (p < 0.001), implying that 27.0% of the variance of the dependent variables was accounted for the H&Y stage. Univariate tests showed that there were significant differences (p < 0.001) across the H&Y stage of all AO variables. The H&Y stage remained significant predictor after controlling for the second variable, the disease duration (p < 0.001, η 2 = 0.249), and there were still significant differences across the H&Y stage of all variables, with effect size (η 2) ranging from 0.132 (p = 0.011) (lnGSH) to the still high values of 0.535 (lnPAB), 0.627 (lnSOD) and 0.964 (lnCAT). Conclusion. The results indicate that higher level of oxidative stress in blood of PD patients is possibly related to the PD stage. Along with reduction of SOD and GSH levels, CAT activity was elevated in comparison to these values in healthy subjects. Furthermore, PAB was shifted toward oxidative stress.

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“…The tissue sample was mixed with 50 mM H 2 O 2 , and the absorbance was measured every 10 s for 3 min at 240 nm. Catalase activity was measured in terms of IU/mg protein by using the standard curve …”

Section: Methodsmentioning

confidence: 99%

Endovascular Stem Cell Therapy Post Stroke Rescues Neurons from Endoplasmic Reticulum Stress-Induced Apoptosis by Modulating Brain-Derived Neurotrophic Factor/Tropomyosin Receptor Kinase B Signaling

Kaur

Sarmah

Veeresh

et al. 2021

ACS Chem. Neurosci.

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Ischemic stroke is devastating, with serious long-term disabilities affecting millions of people worldwide. Growing evidence has shown that mesenchymal stem cells (MSCs) administration after stroke provides neuroprotection and enhances the quality of life in stroke patients. Previous studies from our lab have shown that 1 × 10 5 MSCs administered intra-arterially (IA) at 6 h post stroke provide neuroprotection through the modulation of inflammasome and calcineurin signaling. Ischemic stroke induces endoplasmic reticulum (ER) stress, which exacerbates the pathology. The current study intends to understand the involvement of brain-derived neurotrophic factor/tropomyosin receptor kinase B (BDNF/TrkB) signaling in preventing apoptosis induced by ER stress post stroke following IA MSCs administration. Ischemic stroke was induced in ovariectomized female Sprague Dawley rats. The MSCs were administered IA, and animals were sacrificed at 24 h post stroke. Infarct area, neurological deficit score, motor coordination, and biochemical parameters were evaluated. The expression of various genes and proteins was assessed. An inhibition study was also carried out to confirm the involvement of BDNF/TrkB signaling in ER stress-induced apoptosis. IA-administered MSCs improved functional outcomes, reduced infarct area, increased neuronal survival, and normalized biochemical parameters. mRNA and protein expression of ER stress markers were reduced, while those of BDNF and TrkB were increased. Reduction in ER stress-mediated apoptosis was also observed. The present study shows that IA MSCs administration post stroke provides neuroprotection and can modulate ER stressmediated apoptosis via the BDNF/TrkB signaling pathway.

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Antioxidative enzymes in irradiated rat brain—indicators of different regional radiosensitivity

Cited by 5 publications

References 35 publications

Ameliorative role of Teucrium polium extract on γ-radiation toxicity in brain of albino rats

Ameliorative role of Teucrium polium extract on γ-radiation toxicity in brain of albino rats

Antioxidant status and clinicopathological parameters in patients with Parkinson's disease

Endovascular Stem Cell Therapy Post Stroke Rescues Neurons from Endoplasmic Reticulum Stress-Induced Apoptosis by Modulating Brain-Derived Neurotrophic Factor/Tropomyosin Receptor Kinase B Signaling

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