Antioxidative enzymes in human nasal mucosa after exposure to ozone. Possible role of GSTM1 deficiency

Otto-Knapp, Ralf; Jurgovsky, K; Schierhorn, Katrin; Kunkel, G.

doi:10.1007/s000110300000

Cited by 19 publications

(17 citation statements)

References 41 publications

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“…Cilenšek et al proposed a protective effect for the GSTM1-null genotype against retinopathy [46], explained by an up-regulation of other antioxidant enzymes, such as MnSOD [47]. On the contrary, the result of the aforementioned study is inconsistent with the study that showed a significant correlation between the GSTM1-null genotype and DR [38].…”

Section: Glutathione S-transferases (Gsts)mentioning

confidence: 93%

Oxidative Stress Biomarkers for Diabetic Retinopathy and Medical Management Affecting Oxidative Stress

Cilenšek¹,

Ramuš²,

Petrovič³

et al. 2016

Role of Biomarkers in Medicine

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Changes in dietary habits and lifestyles associated with rapid economic growth have dramatically increased the incidence of diabetes and related vascular complications. Diabetic retinopathy (DR), a microvascular complication of diabetes, is associated with both environmental and genetic factors. Several metabolic abnormalities are implicated in its pathogenesis; however, the exact mechanism remains to be determined. Among them, oxidative stress is expected to play an important role.Environmental, genetic, and epigenetic factors affecting the oxidative stress responsible for DR are reviewed in this paper. The knowledge about genetic biomarkers of DR is quite extensive, whereas the awareness about epigenetics and epigenetic markers is only beginning to be understood.Modulation of epigenetic changes by pharmaceutical means may provide a potential strategy to retard the progression of DR. In addition to the intense medical management, these strategies include dietary measures (antioxidants) and the introduction of epigenetic drugs, such as inhibitors of DNA methylation and histone demethylases.

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Section: Glutathione S-transferases (Gsts)mentioning

confidence: 93%

Oxidative Stress Biomarkers for Diabetic Retinopathy and Medical Management Affecting Oxidative Stress

Cilenšek¹,

Ramuš²,

Petrovič³

et al. 2016

Role of Biomarkers in Medicine

View full text Add to dashboard Cite

show abstract

“…Therefore, SOD activity is calculated from the time-dependent difference between the MTT assay containing and not containing the homogenate. GPx was analysed using a modification of the coupled enzyme assay by Otto-Knapp et al (2003). GPx catalyses the oxidation of reduced glutathione (GSH, 0.1 mM) to oxidised glutathione (GSSG) accompanied with the reduction of H 2 O 2 (0.05%) to H 2 O.…”

Section: Methodsmentioning

confidence: 99%

“…The GPx activity was finally determined by following the oxidation of NADPH (0.15 mM) to NADP at 340 nm within the GR (1 U/ml) catalysed reduction of GSSG to GSH. GR was analysed according to Otto-Knapp et al (2003) by following the oxidation of NADPH (0.3 mM) to NADP at 340 nm within the GR catalysed reduction of GSSG (2 mM) to GSH.…”

Section: Methodsmentioning

confidence: 99%

Mitochondrial respiratory and antioxidative enzyme activities in broiler meat in relation to age and gender of the animals

Werner

Janisch

Wicke

2011

Animal

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Colour is an important quality parameter of broiler meat influencing the consumer buying behaviour. The alterations of the colour after slaughter are related to the oxidative status of the tissue. This in turn is influenced by an interaction between the mitochondria and the antioxidative enzymes. In this study, breast muscles were collected from hens and cocks of a commercial line slaughtered at the ages of 28 and 41 day. Analysis of the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GR) was performed with samples obtained 20 min and 48 h after slaughter (post mortem, p.m.), whereas the mitochondrial respiratory activity was analysed in permeabilised breast muscle fibres collected 20 min p.m. The carcass characteristics of breast muscle and leg weight as well as breast yield were significantly higher, and the leg yields lower, in the 41-day-old broiler. The 28-day-old hens and cocks had comparable carcass characteristics ( P . 0.05), whereas 41-day-old cocks had significantly higher carcass, breast and leg weight in comparison to the hens. The pH 20 min p.m. and the L* 48 h p.m. were significantly higher, and the a* and b* values of the 20 min and 48 h p.m. samples as well as the drip loss were significantly lower in the 41-day-old broiler. Mitochondrial respiratory rates were comparable ( P . 0.05) between the 28-and 41-day-old cocks and hens. The same result could be found with regard to the activities of the SOD, GPx and GR except for lower activities of the SOD 20 min p.m. and higher of the GR 48 h min p.m. in the 41-day-old broiler. The concentrations of thiobarbituric acid-reactive substances were generally higher in the breast muscles of the 41-day-old broiler. Assorting the data according to their mean pH 20 min p.m. indicates a positive influence of higher pH values (.6.34) on the mitochondrial function, whereas a low pH 20 min p.m. results in tendentially and significantly higher activities of the antioxidative enzymes and drip loss values. These results indicate a relation between the meat quality and the oxidative metabolism as well as antioxidative capacity of the meat.

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“…Therefore, SOD activity is calculated from the time-dependent difference between the MTT assay containing and not containing the homogenate. GPx was analysed using a modification of the coupled enzyme assay by Otto-Knapp et al (2003). Within the test, GPx catalysed the H 2 O 2 (0.05%)-dependent oxidation of reduced (glutathione, GSH; 0.1 mM) to oxidised glutathione (GSSG).…”

Section: Animals and Sample Collectionsmentioning

confidence: 99%

mentioning

confidence: 99%

Mitochondrial respiratory and antioxidative enzyme activities in turkey meat

Janisch

Wicke

Krischek

2012

Animal

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Meat quality and (anti)oxidative metabolism of m. pectoralis superficialis (MPS), m. gastrocnemius (MG) and m. iliotibilialis lateralis (MIL) from turkey toms were analysed. After slaughter, pH of MPS and MG decreased and electrical conductivity of the MPS increased. The MG had generally higher pH values. The meat lightness (L*) and redness (a*) increased in MG and MPS after slaughter. The MPS always had higher L* and lower a* values. Mitochondrial respiratory activities (MRA) were higher in the MIL than the MPS. The activities of superoxide dismutase (SOD) and glutathione peroxidase, analysed in the MPS, increased and the glutathione reductase activity decreased after slaughter. Meat samples with lower pH 24 h p.m. had higher drip loss and L* values. The MRA were tendentially lower and the SOD activities higher in these samples. These results indicate a relation between the meat quality, the antioxidative metabolism and mitochondrial respiration.Keywords: turkey, mitochondrial respiration, antioxidative enzyme activities, meat quality ImplicationsAn increasing amount of turkey breast meat is sold in selfservice packages. Because of this, meat colour differences between meat pieces or higher drip loss are more obvious to consumers, which is one of the reasons for increasing complaints. The muscle-to-meat transition is a complex process, characterised by a decrease in pH and a change in the oxidative status, revealing the simultaneous production of reactive oxidative substances by mitochondria and the activation of the corresponding antioxidative enzymes. The aim of this study was to elucidate the changes in these oxidative processes after animal slaughter in relation to meat quality differences. IntroductionAlthough the colour variation of poultry meat under modified atmospheric conditions could be, to a certain degree, physiologically explicable (Werner et al., 2009), at the time of purchase consumers show concern, especially if meat is characterised by a heterogeneous appearance, poor texture and cohesiveness, and a higher drip loss (Barbut, 2009). This meat is referred to as pale, soft and exudative (PSE)-like meat with similarities to PSE pork (Barbut, 2009;Petracci et al., 2009). Colour of meat is related to the total myoglobin (Mb) content and the proportion of the Mb redox forms oxy-, deoxy-and met-Mb. Total Mb content is influenced by gender, genetics, age and, within a species, muscle type. An important feature is the fibre-type composition of the muscles, with breast muscles only consisting of fast-twitch glycolytic (FTG) fibres containing low Mb and mitochondria concentrations, and leg muscles additionally containing Mb and mitochondria-rich slow-twitch oxidative (STO) fibres (Papinaho et al., 1996;Mancini and Hunt, 2005). Proportions of oxy-, deoxy-and metMb are strongly related to the oxidative status of the tissue influenced by the oxygen consumption and the antioxidative capacity of the tissue (Figueiredo et al., 2008). The electron transport chain (ETC) of mitochondria is not only responsible for...

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Antioxidative enzymes in human nasal mucosa after exposure to ozone. Possible role of GSTM1 deficiency

Abstract: Our findings suggest that GSTM1-deficiency has an impact on AOE-regulation after ozone exposure.

Cited by 19 publications

References 41 publications

Oxidative Stress Biomarkers for Diabetic Retinopathy and Medical Management Affecting Oxidative Stress

Oxidative Stress Biomarkers for Diabetic Retinopathy and Medical Management Affecting Oxidative Stress

Mitochondrial respiratory and antioxidative enzyme activities in broiler meat in relation to age and gender of the animals

Mitochondrial respiratory and antioxidative enzyme activities in turkey meat

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