Antioxidative effects of tannic acid, cacao oil and st. john’s wort oil on the oxidative stress induced by cadmium in packed human erythrocytes

Abstract

Cadmium (Cd) is among the heavy metals causing environmental pollution and can cause temporary or permanent oxidative stress in the cells. Antioxidative enzyme mechanisms may be inadequate in the response of living organisms to oxidative stress caused by these toxic substances. In this study, in order to prevent Cd induced oxidative stress, the possible protective effects of (TA), cacao oil (CO) and St. John's Wort oil (JWO) on lipid erythrocytes and antioxidant enzymes were investigated. We prepared packed erythrocytes from the blood samples of 7 healthy volunteers. Experiment groups consisting of control, Cd, TA, CO, JWO and their combinations (Cd+TA, Cd+CO ve Cd+JWO) were arranged. In all groups, malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) were measured. Comparisons between groups of normally distributed variable were evaluated by One-Way variance analysis (ANOVA). Comparisons between groups of not normally distributed variable wereevaluated by Kruskal-Wallis test. After Cd administration SOD activity was increased in TA (3507± 68.2 u/gHb), CO (3518 ±170.0 u/gHb) and JWO (3469±249.5 u/ gHb) groups compared to Cd group. After Cd administration MDA levels were decreased in CO (52,1±24,3 nmol/gHb) and JWO (54,1±23,7 nmol/gHb) groups compared to Cd group. However, it did not change in the TA (61,5±50,0 nmol/gHb) group. After Cd administration CAT activity was increased in TA (228,2±31,3 u/gHb), CO (281,6±295,3 u/gHb) and JWO (267,8±69,4 u/gHb) groups compared to Cd group (196,2±223,0 u/gHb). These results indicate that HF and SCI may reduce Cd-induced oxidative stress, but TA is not very effective in terms of MDA levels. These data obtained in terms of other studies may be guiding.

Examination of some quality analysis carried out to the determination of standardization of St. John's Wort (Hypericum perforatum L.) oil used in traditional medicine applications

“…Sarı kantaron bitkisinin çiçek ve yaprakları ezildiğinde çıkan kırmızı renk ile maserasyon yoluyla yapılan yağdaki kırmızı rengin hiperisinden kaynaklandığı bilinmektedir [4]. Yapılan çalışmalarda sarı kantaron bitkisinin; yara ve yanık tedavileri, uyku düzensizlikleri, anksiyete, depresyon tedavisi, doğal kaynaklı ve orta dereceli antidepresan [14; 15], antimikrobiyal, anti-viral, anti-bakteriyel, antioksidan [16,17], antiinflamatuar, antiülser, anti-tümoral [18], antioksidatif [19], miyalji, kurt düşürücü, rektum iltihapları [20] tedavisinde kullanıldığı bildirilmiştir.…”