Antioxidative effect of Periplaneta americana extract on dextran sulfate sodium-induced ulcerative colitis through activation of the Nrf2 signal

Abstract

Context Periplaneta americana L. (Blattariae) is used as a treatment for ulcerative colitis (UC) in Chinese traditional medicine. Objective To evaluate the antioxidative activity of P. americana whole body ethanol extract (PAE) on UC mice and whether glycine and proline could be used for quality control and identification of active PAE components. Materials and methods NCM460 cells were pre-incubated in PAE, AA-L, AA-M, and AA-H (low, high and medium doses of proline and glycine), then treated with recombinant human TNF-α. The glutathione (GSH), malondialdehyde (MDA), superoxide dismutase (SOD) and reactive oxygen (ROS) levels were determined. UC mice were fed with water containing 2.5% dextran sulfate sodium (w/v) after pre-treatment with different doses of PAE once a day for 7 days. ELISA was used to detect the concentrations of inflammation-related factors. Colon tissues of mice were used to detect the activity of myeloperoxidase (MPO), GSH, MDA, and SOD. Histological changes were observed using H&E staining. The expression of target proteins was determined by western blotting. Results In vivo , PAE treatment reduced the DAI score more than in the model group, restoring the weight and colonic length. It also reduced the severity of colitis, and inflammatory and oxidative stress intensity. Additionally, western blotting showed that the Nrf2 pathway was activated by PAE. In vitro PAE significantly alleviated TNF-α-induced cell damage and oxidative stress, which is relevant to the activation of the Nrf2 pathway. Conclusions PAE may relieve oxidative stress through the Nrf2 signaling pathway, and proline and glycine may be used as active components of its antioxidative stress activity.

Polygonatum cyrtonema polysaccharides reshape the gut microbiota to ameliorate dextran sodium sulfate-induced ulcerative colitis in mice

“…In this study, PCPs significantly ameliorated the symptoms of weight loss, colon length shortening, increased liver and spleen weight indices, and high DAI scores in UC mice. Oxidative stress is a state of imbalance between oxidative processes and antioxidant defenses in the body, leading to neutrophil infiltration, protease secretion increase, intestinal mucosal damage, and exacerbation of UC symptoms ( Wu et al, 2023 ). Moreover, abnormal inflammation can disrupt the anti-inflammatory system in the intestine, and chronic inflammation in the body eventually leads to colon tissue fibrosis, enhancing the progression of UC ( D'Alessio et al, 2022 ; Shao et al, 2019 ).…”

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“…In this study, PCPs significantly ameliorated the symptoms of weight loss, colon length shortening, increased liver and spleen weight indices, and high DAI scores in UC mice. Oxidative stress is a state of imbalance between oxidative processes and antioxidant defenses in the body, leading to neutrophil infiltration, protease secretion increase, intestinal mucosal damage, and exacerbation of UC symptoms (Wu et al, 2023). Moreover, abnormal inflammation can disrupt the anti-inflammatory system in the intestine, and chronic inflammation in the body eventually leads to colon tissue fibrosis, enhancing the progression of UC (D'Alessio et al, 2022;Shao et al, 2019).…”