Antioxidative Effect and Tyrosinase Inhibitory Activity of the Unripened Fruit Extract of Rubus coreanus Miquel

Abstract

요 약: 본 연구에서는 미성숙 복분자 추출물의 항산화 효능과 타이로시네이즈 저해 활성을 평가하고 이들의 기 능성 화장품 원료로서의 응용 가능성을 확인하였다. 모든 실험은 미성숙 복분자의 50% 에탄올 추출물, 에틸아 세테이트 분획, 아글리콘 분획을 이용하여 진행하였다. DPPH (1,1-diphenyl-2-picrylhydrazyl)법을 이용한 자유 라디칼 소거 활성(FSC50)은 에탄올 추출물(6.56 μg/mL)과 에틸아세테이트 분획(6.14 μg/mL)이 대표 적인 지용성 항산화제인 (+)-α-tocopherol (8.98 μg/mL)보다 높은 것으로 나타났다. Fe 3+-EDTA/H2O2 계에서 생성된 활성산소종에 대한 소거 활성(OSC50)은 에탄올 추출물(0.83 μg/mL), 에틸아세테이트 분획 (0.84 μg/mL), 아글리콘 분획(1.13 μg/mL) 세 분획 모두 대표적인 수용성 항산화제인 L-ascorbic acid (1.5 μg/mL)보다 높은 것으로 나타났다. Rose bengal로 광증감된 1 O2에 의한 적혈구 파괴에 대하여, 세 분획 모두 농도 의존적(1 ~ 50 μg/mL)인 세포보호 활성을 나타내었다. 또한 50 μg/mL 농도를 기준으로 비교하였 을 때, 50% 에탄올 추출물의 τ50은 296.3 min으로 가장 높은 세포보호 활성을 나타내었다. 타이로시네이즈 저해 활성에서 에틸아세테이트 분획과 아글리콘 분획은 알부틴보다 높은 저해 효과를 나타내었다. 이상의 결과 들로부터 미성숙 복분자 추출물은 라디칼을 포함한 활성산소종을 소거하는 항산화제로서, 알부틴을 대체하는 미백 기능성 원료로서 응용될 수 있음을 확인하였다.Abstract: In this study, the antioxidative effects and inhibitory activities of unripened fruit extract of Rubus coreanus Miquel (R. coreanus Miquel) on tyrosinase were investigated and the potential applicability as a cosmeceutical ingredients was evaluated. All experiments were performed with 50% ethanol extract, ethyl acetate fraction and aglycone fraction of unripened fruit extract of R. coreanus Miquel. The DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activites (FSC50) of 50% ethanol extract (6.56 μg/mL) and ethyl acetate fraction (6.14 μg/mL) of unripened fruit extract of R. coreanus Miquel were higher than (+)-α-tocopherol (8.98 μg/mL), which is known as a typical hydrophobic antioxidant. Reactive oxygen species (ROS) scavenging activities (OSC50) of 50% ethanol extract (0.83 μg/mL), ethyl acetate fraction (0.84 μg/mL) and aglycone fraction (1.13 μg/mL) of R. coreanus Miquel on ROS generated in Fe 3+ -EDTA/H2O2 system were higher than L-ascorbic acid (1.5 μg/mL), which is known as s typical hydrophilic antioxidant. The cellular protective effect of extract and fraction of unripened fruit extract of R. coreanus Miquel on the rose bengal sensitized photohemolysis of human erythrocytes was increased in a concentration dependent manner (1 ~ 50 μg/mL). And 50% ethanol extract in 50 μg/mL showed the most protective effect among extracts (τ50 = 한샛별⋅권순식⋅공봉주⋅김경진⋅박수남 대한화장품학회지, 제 39권 제 4 호, 2013 296.3 min). The inhibitory effects on tyrosinase of ethyl acetate and agylcone fractions were higher than arbutin. These results indicate that unripened fruit extracts of R. coreanus Miquel can be applied to antioxidant scavenging ROS including radical as an alternative whitening agent to replace arbutin.

Licoricidin, an isoflavonoid isolated from Glycyrrhiza uralensis Fisher, prevents UVA‐induced photoaging of human dermal fibroblasts

“…This model detects most ROS (, · OH and H 2 O 2 ), with the exception of 1 O 2 . When luminol is oxidized, luminescence is emitted (420–450 nm); therefore, ROS scavenging compounds reduce the luminescence output . The scavenging activities of licoricidin and a reference compound are shown in Fig.…”

“…Human erythrocytes are an acceptable cell type that can be used to measure the effects of ROS on lipid peroxidation, protein oxidation, destruction of antioxidants and haemolysis of cells. In addition, as erythrocytes isolated from humans can be used, data on the antioxidant activity of compounds can be extrapolated to clinical settings . Using rose bengal as a sensitizer to ROS‐dependent cellular damage, we evaluated whether licoricidin exhibited any protective effects.…”

Inhibitory effects of mung bean (Vigna radiata L.) seed and sprout extracts on melanogenesis

“…The follow-up reaction after the 2 oxidation reactions occurs spontaneously. Therefore, the reaction catalyzed by tyrosinase determines the overall reaction rate, and inhibition of tyrosinase activity is an important factor for evaluation of depigmentation effects (33). Inhibitory activities of mung bean seed and sprout extract fractions on in vitro tyrosinase activity are shown in Fig.…”

Effectiveness of maturity of Rubus occidentalis on hyperalgesia induced by acidic saline injection in rats

“…Taghi et al found that ellagic acid had central and peripheral antinociceptive effect in experimental pain model [ 19 ], which supports the analgesic effect of immature R. occidentalis based on our findings. In addition, R. coreanus fruit have potential applications owing to their antioxidative effects mediated via the scavenging of reactive oxygen species [ 20 ]. Therefore, it is suggested that components with the observed antioxidative activity are degraded during the ripening process.…”