Antioxidative Defense and Gut Microbial Changes under Pollution Stress in Carassius gibelio from Bucharest Lakes

Abstract

Fish are able to accumulate by ingestion various contaminants of aquatic environment, with negative consequences on their intestine, being continuously threatened worldwide by heavy metals, pesticides and antibiotics resulted from the human activities. Consequently, the health of other species can be affected by eating the contaminated fish meat. In this context, our study aimed to perform a comparison between the changes in intestine samples of Carassius gibelio individuals collected from different artificial lakes in Bucharest (Romania), used by people for leisure and fishing. The presence of various metals, pesticides and antibiotics in the gut of fish was assessed in order to correlate their accumulation with changes of antioxidative enzymes activities and microbiome. Our results showed that fish from Bucharest lakes designed for leisure (Chitila, Floreasca and Tei lakes) have an increased level of oxidative stress in intestine tissue, revealed by affected antioxidant enzymes activities and GSH levels, as well as the high degree of lipid peroxidation, compared to the fish from protected environment (Vacaresti Lake). Some heavy metals (Fe, Ni and Pb) and pesticides (aldrin and dieldrin) were in high amount in the gut of fish with modified antioxidative status. In conclusion, our study could improve the knowledge regarding the current state of urban aquatic pollution in order to impose several environmental health measures.

The COVID-19 Pandemic Impact of Hospital Wastewater on Aquatic Systems in Bucharest

“…WWTPs have been hotspots for increasing the populations of antibiotic-resistant bacteria, especially during the COVID-19 pandemic, triggering a wide range of consequences such as contamination of wastewater treatment plant workers [30]. In addition, their contamination with antibiotic-resistant bacteria could increase the chances of nosocomial diseases generation once these workers or their first contact relatives come in contact with healthcare units.…”

Effects on grass carp ( Ctenopharyngodon idella ) induced by waterborne lead exposure and the association with alteration of microbiota in the intestine and water

“…It was found that sediment physicochemical factors and HMs co‐drove microbial variation, but HMs continue to be the main driving factors of fungal communities (Yao et al, 2022). When compared to fish from Bucharest lakes designed for leisure (Chitila, Floreasca and Tei lakes), which experienced increased levels of oxidative stress based on the examination of their intestinal tissues, fish from a protected environment (Vacaresti lake) had a modified antioxidative status due to increased levels of HMs (Fe, Ni and Pb) in their intestines (Alistar et al, 2022). In bighead carp ( Hypophthalmichthys nobilis ), Pb may lead to the destruction of the intestinal barrier, leading to a disordered intestinal microbial community, which in turn promotes the occurrence of cell apoptosis (Liu et al, 2020).…”

“…In bighead carp ( Hypophthalmichthys nobilis ), Pb may lead to the destruction of the intestinal barrier, leading to a disordered intestinal microbial community, which in turn promotes the occurrence of cell apoptosis (Liu et al, 2020). There are many related studies on the ways that HMs cause harm to fish (Alistar et al, 2022; Liu et al, 2020; Shahjahan et al, 2022). However, the interactions between environmental and intestinal microbiota in cultured fish require further investigation, especially in aquaculture.…”