Antioxidative Capacity Of Melatonin In Follicular Fluid Of Aged IVF Patients: Beneficial Effects On Oocytes And Embryo

Abstract

The aim of the present study was to evaluate the role of melatonin supplementation on the main IVF outcomes in aged patients underwent IVF. 358 infertile women aged over 40 underwent a shortdown-regulation protocol and were randomized into two groups: 178 patients who received 5mg melatonin (group A) and 180 patients who did not received melatonin (group B). Oxidative stress values, mature oocytes, embryo quality, pregnancy rates and implantation rates, intrafollicular concentration of melatonin and progesterone were measured.There were significant statistical differences comparing group A with group B in terms of mature oocytes (48.2% vs 35.0%), oxidative stress (CARR U) (190 ± 41 vs 388 ± 64), antioxidative capacity (AOCs) (1,76±0,4 vs 0,89 ± 0,2), progesterone concentration in follicular fluid (10,4±1.1 ml vs 4,3±0,8 ml) and grade I embryos (45,7% vs 30,4%, p=0.0045).Melatonin intrafollicular concentrations were significantly increased after melatonin treatment (213±35 pg/ml versus 69 ± 23 pg/ml). In conclusion melatonin supplementations during ovarian stimulationin aged patients improve oocyte and embryo quality, increasing progesterone production and scavenging free radicals. Furthermorewe demonstrated that exogenous administrated melatonin is able to accumulate efficiently in the follicular fluid.

Inositol supplement improves clinical pregnancy rate in infertile women undergoing ovulation induction for ICSI or IVF-ET

“…Many studies have already investigated and proposed potential predictive factors for negative and positive IVF-ET outcomes. [ 23 , 24 ] Confirmed by a previous meta-analysis, female age, duration of subfertility, and basal FSH level are also predictors of pregnancy after IVF-ET. [ 25 ] Attempt of MI administration in IVF-ET has lasted for nearly 2 decades.…”

The lipid peroxidation product 4-hydroxynonenal contributes to oxidative stress-mediated deterioration of the ageing oocyte

“…Moreover, it has also been reported that antioxidant therapy can assist with the recovery of oocyte quality following oxidative insult over shorter periods of time. Indeed, administration of melatonin during human ovarian stimulation in preparation for IVF reduced OS parameters and improved blastocyst quality and development 94 . Additionally, media supplementation with the antioxidants cystine and cysteamine during IVM resulted in decreased aneuploidy and improved embryo development after maternal restraint stress-induced OS 95 and maternal ageing 96 .…”

Molecular Mechanisms Responsible for Increased Vulnerability of the Ageing Oocyte to Oxidative Damage

“…In a clinical context, supplementation of the antioxidant melatonin during ovarian stimulation in women undergoing IVF resulted in efficient accumulation into follicular fluid and improved oocyte and embryo quality in aged women [ 223 ]. In mice, the long-term dietary supplementation of melatonin, between 10 and 43 weeks of age, was also able to mitigate the phenotype of the ageing oocyte with the restoration of oocyte numbers, fertilisation rate, and blastulation rate.…”