Antioxidative and in vitro antiproliferative activity of Arctium lappa root extracts

Abstract

BackgroundArctium lappa, known as burdock, is widely used in popular medicine for hypertension, gout, hepatitis and other inflammatory disorders. Pharmacological studies indicated that burdock roots have hepatoprotective, anti-inflammatory, free radical scavenging and antiproliferative activities. The aim of this study was to evaluate total phenolic content, radical scavenging activity by DPPH and in vitro antiproliferative activity of different A. lappa root extracts.MethodsHot and room temperature dichloromethanic, ethanolic and aqueous extracts; hydroethanolic and total aqueous extract of A. lappa roots were investigated regarding radical scavenging activity by DPPH, total phenolic content by Folin-Ciocalteau method and antiproliferative in vitro activity was evaluated in human cancer cell lines. The hydroethanolic extract analyzed by high-resolution electrospray ionization mass spectroscopy.ResultsHigher radical scavenging activity was found for the hydroethanolic extract. The higher phenolic contents were found for the dichloromethane, obtained both by Soxhlet and maceration extraction and hydroethanolic extracts. The HRESI-MS demonstrated the presence of arctigenin, quercetin, chlorogenic acid and caffeic acid compounds, which were identified by comparison with previous data. The dichloromethane extracts were the only extracts that exhibited activity against cancer cell lines, especially for K562, MCF-7 and 786-0 cell lines.ConclusionsThe hydroethanolic extracts exhibited the strongest free radical scavenging activity, while the highest phenolic content was observed in Soxhlet extraction. Moreover, the dichloromethanic extracts showed selective antiproliferative activity against K562, MCF-7 and 786-0 human cancer cell lines.

In vitro Analysis of Antioxidant, Antimicrobial and Antiproliferative Activity of Enteromorpha antenna, Enteromorpha linza and Gracilaria corticata Extracts

“…A graph was plotted with concentration of the drug versus relative cell viability. The cell viability was calculated using the formula below (20, 21):…”

Effect of flavonoids content on antioxidant activity of commercial cosmetic plant extracts

“…In turn sophora japonica, known as anti-tyrosinase extract, was especially potent in HEMn cells in terms of free radical scavenging effects [21,22]. There are also scientific reports indicating that Arctium lappa extracts are the strong free radical scavengers and exhibit selective antiproliferative activity [23][24][25]. Medicinal uses of burdock in treating chronic diseases such as cancers and diabetes have been reported [26].…”

Influence of Roasting Treatment on the Antioxidant Activities and Color of Burdock Root Tea

“…Among the parts of the burdock plant, roots are commonly consumed as a side dish and in the form of tea in Korea. Many studies also suggest that burdock roots possess antioxidative, anti-inflammatory, and anti-cancer activities (8–10). These biological activities are attributed to the presence of polyphenols as well as dietary fiber such as inulin and lignan in burdock roots (1,3).…”