Antioxidative and immunogenic properties of catalase-peroxidase protein in<i>Penicillium marneffei</i>

Pongpom, Monsicha; Sawatdeechaikul, Pritsana; Kummasook, Aksarakorn; Khanthawong, Sophit; Vanittanakom, Nongnuch

doi:10.3109/13693786.2013.807445

Cited by 14 publications

(20 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was shown when T. marneffei cells were spotted on a medium containing H 2 O 2 and the fungus allowed to grow at either 25°C or 37°C for 5–7 days. Survival occurred only on media containing less than 3 mM of H 2 O 2 [ 45 ]. In contrast, other pathogenic fungi demonstrated very high resistance to this chemical.…”

Section: Resistance To Oxidative Stress By T Marneffeimentioning

confidence: 99%

“…Additionally, T. marneffei could secrete antioxidant molecules to neutralize ROS/RNS toxicity. A significant upregulation of a transcript encoding for catalase-peroxidase (CpeA) bifunctional enzyme by the yeast form of T. marneffei is shown in the yeast culture and during infection in murine macrophages [ 34 , 45 ]. Thus, this enzyme may play an important role by limiting the effect of H 2 O 2 during infection.…”

Section: Resistance To Oxidative Stress By T Marneffeimentioning

confidence: 99%

“…Two genes encoding for antioxidant proteins have been reported. They include superoxide dismutase ( sodA ) [ 69 ] and catalase-peroxidase ( cpeA ) [ 34 , 45 ]. These antioxidant proteins have been shown to aid resistance to phagocytic killing by fungal pathogens such as H. capsulatum and C. neoformans [ 70 , 71 ].…”

Section: Proteins Associated With the Phagosome-mimic Stress Responsementioning

confidence: 99%

“…When a cpeA deletion mutant and the wild-type strain were used to infect a THP-1 macrophage cell line, the mutant exhibited a decreased ability to survive. This result suggested that the CpeA plays a role in the resistance to phagocytic killing [ 45 ].…”

Section: Proteins Associated With the Phagosome-mimic Stress Responsementioning

confidence: 99%

See 3 more Smart Citations

Adaptation to Macrophage killing by Talaromyces Marneffei

et al. 2017

Self Cite

View full text Add to dashboard Cite

Talaromyces (Penicillium) marneffei is an important opportunistic fungal pathogen. It causes disseminated infection in immunocompromised patients especially in Southeast Asian countries. The pathogenicity of T. marneffei depends on the ability of the fungus to survive the killing process and replicate inside the macrophage. Major stresses inside the phagosome of macrophages are heat, oxidative substances and nutrient deprivation. The coping strategies of this pathogen with these stresses are under investigation. This paper summarizes factors relating to the stress responses that contribute to the intracellular survival of T. marneffei. These include molecules in the MAP signal transduction cascade, heat shock proteins, antioxidant enzymes and enzymes responsible in nutrient retrieval. There is speculation that the ability of T. marneffei to withstand these defenses plays an important role in its pathogenicity.

show abstract

Section: Resistance To Oxidative Stress By T Marneffeimentioning

confidence: 99%

Section: Resistance To Oxidative Stress By T Marneffeimentioning

confidence: 99%

Section: Proteins Associated With the Phagosome-mimic Stress Responsementioning

confidence: 99%

Section: Proteins Associated With the Phagosome-mimic Stress Responsementioning

confidence: 99%

See 2 more Smart Citations

Adaptation to Macrophage killing by Talaromyces Marneffei

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…To test the chronic effects of H 2 O 2 treatment on mycelia and yeast cell growth, 5 μl of conidial suspension (1 × 10 5 −1 × 10 2 conidia/ml) of WT, Δ madsA and CMA strains was spotted on the surface of the SDA plate supplemented with 1.25, 2.5, 5, 10, or 20 mM H 2 O 2 , respectively. Then, the conidia were cultured on SDA plates for 7 d at 25°C or 37°C (Pongpom et al, 2013 ).…”

Section: Methodsmentioning

confidence: 99%

MADS-Box Transcription Factor MadsA Regulates Dimorphic Transition, Conidiation, and Germination of Talaromyces marneffei

Wang

et al. 2018

Front. Microbiol.

View full text Add to dashboard Cite

The opportunistic human pathogen Talaromyces marneffei exhibits a temperature-dependent dimorphic transition, which is closely related with its pathogenicity. This species grows as multinucleate mycelia that produce infectious conidia at 25°C, while undergoes a dimorphic transition to generate uninucleate yeast form cells at 37°C. The mechanisms of phenotype switching are not fully understood. The transcription factor madsA gene is a member of the MADS-box gene family. Previously, it was found that overexpression of madsA gene resulted in mycelial growth instead of yeast form at 37°C. In the current study, the madsA deletion mutant (ΔmadsA) and complemented strain (CMA) were constructed by genetic manipulation. We compared the phenotypes, growth, conidiation, conidial germination and susceptibility to stresses (including osmotic and oxidative) of the ΔmadsA with the wild-type (WT) and CMA strains. The results showed that the ΔmadsA displayed a faster process of the yeast-to-mycelium transition than the WT and CMA. In addition, the deletion of madsA led to a delay in conidia production and conidial germination. The tolerance of ΔmadsA conidia to hydrogen peroxide was better than that of the WT and CMA strains. Then, RNA-seq was performed to identify differences in gene expression between the ΔmadsA mutant and WT strain during the yeast phase, mycelium phase, yeast-to-mycelium transition and mycelium-to-yeast transition, respectively. Gene ontology functional enrichment analyses indicated that some important processes such as transmembrane transport, oxidation-reduction process, protein catabolic process and response to oxidative stress were affected by the madsA deletion. Together, our results suggest that madsA functions as a global regulator involved in the conidiation and germination, especially in the dimorphic transition of T. marneffei. Its roles in the survival, pathogenicity and transmission of T. marneffei require further investigation.

show abstract